AL 01- Genomics and Proteomics (UnB, Embrapa Cenargen)
Team Leader: Robert N. G. Miller
Laboratory Activities with INCT PlantStress Biotech
Integration data from monocotyledon transcriptome (rice, corn, wheat, Musa and sorghum) submitted to water deficit, generated by mass sequencing (Illumina - HiSeq) in previous projects.
Integration of transcriptome data from drought-tolerant genotypes of Musa spp., Arachis spp. and cowpea subjected to water deficit combined with biotic stress (Meloidogyne spp. or Mycosphaerella) in bioassays.
Integration and sequencing of transcripts of fungus-tolerant genotypes in c subjected to multiple stresses (Musa X Mycosphaerella musicola and Fusarium oxysporun).
Validate the expression of key genes in the metabolic pathways of plants' response to combined stresses (biotic-biotic; biotic-biotic; abiotic-biotic) by qRT-PCR.
Sequence through the Illumina platform a fraction of small RNAs, and their target mRNAs, and circular plant RNAs (Arachis, Musa, soybean, pitangueira, and cashew) subjected to biotic and/or abiotic stresses.
Validate the function of plant genes potentially involved in drought tolerance mechanisms in Arabidopsis, rice or Setaria plants by overexpression or silencing strategies.
Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members.
The Laboratory Genomics and Proteomics carries out studies aimed at prospecting and identifying new genes, regulatory sequences and molecules involved with the resistance/tolerance responses of plants to biotic and abiotic stresses, aiming at the elucidation of the molecular mechanisms of plants that occur in response to different stresses, combined or not. The research group also performs the validation of function of these assets in model plants.
• Prospecting for genes/molecules of interest for pest and drought control in wild germplasm of Musa spp.
• Prospecting of target molecules in phytoparasitic nematodes (Meloidogyne spp.) and phytopathogenic fungi (Pseudocercospora spp.) for pest control.
• Prospecting for small RNAs in plant genotypes that are resistant/tolerant to pests and drought that may be involved in responding to these stresses.
• Prospecting for genes/molecules that are efficient in pest control and tolerance to water deficit simultaneously (cross-stress).
• Validation of prospective innovation assets by their overexpression or gene silencing in model plants for analysis and validation of their function.
Robert Neil Gerard Miller
Graduated in Biological Sciences - Manchester Metropolitan University, UK (1990), Master in Plant Protection - University Of Bath, UK (1991) and PhD in Molecular Biology and Phytopathology - University of Reading, UK (1995). He is currently Associate Professor I at the University of Brasilia (Campus Darcy Ribeiro, Department of Cellular Biology), supervising the Graduate Programs in Molecular Biology, Phytopathology and Microbial Biology. He worked between 2014 and 2016 as Coordinator of the Graduate Program in Molecular Biology (CAPES concept 6). He works as editor for the journals Annals of Botany and Tropical Plant Pathology, he is coordinator of national and international projects, mainly in the following themes: functional genomics of plants and microorganisms; search for genes for resistance to biotic stress in plants; and characterization of phytopathogenic and mycotoxigenic fungi.
Robert Neil Gerard Miller
Microbiology Laboratory: Plant-Prague Interaction, Bloco I-1-35/8,
Institute of Biological Sciences, Department of Cell Biology,
Universidade de Brasília, Campus Universitário Darcy Ribeiro, Asa Norte, Postal Code 70910-900, Brasília, DF, Brazil.