AL 03 - Molecular Genetics - (UFRJ, Embrapa Cenargen)

Team Leader: Márcio Alves Ferreira

Laboratory Activities with INCT PlantStress Biotech

Integration of the transcriptome and sequencing of the model plant (Setaria) submitted to drought and high carbon dioxide content.

Validate the expression of key genes in the metabolic pathways of plants response to combined stresses (biotic-biotic; biotic-biotic; abiotic-biotic) by qRT-PCR.

Identify in silico promoters responsive to biotic and abiotic stress (drought and phytonematodes), with activity in different organs (root and leaf).

Set up a vector bank with promoters directing the expression of reporter genes (GUS/GFP) in monocots and dicots.

Validate promoters by transient transformation by biolistics or Agrobacterium rhyzogenes in target plants for in vivo testing of promoters (soybean, cotton and corn).

Analyze the sequencing data to check the methylation status of gene promoters in the metabolic pathways of interest.

Validate the function of plant genes potentially involved in drought tolerance mechanisms in Arabidopsis, rice or sepia plants through strategies of overexpression or silencing.

Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members.