AL 03 - Molecular Genetics
Laboratory Activities with PlantStress Biotech INCT
Integration of the transcriptome and sequencing of the model plant (Setaria) submitted to drought and high carbon dioxide content.
Validate the expression of key genes in the metabolic pathways of plants response to combined stresses (biotic-biotic; biotic-biotic; abiotic-biotic) by qRT-PCR.
Identify in silico promoters responsive to biotic and abiotic stress (drought and phytonematodes), with activity in different organs (root and leaf).
Set up a vector bank with promoters directing the expression of reporter genes (GUS/GFP) in monocots and dicots.
Validate promoters by transient transformation by biolistics or Agrobacterium rhyzogenes in target plants for in vivo testing of promoters (soybean, cotton and corn).
Analyze the sequencing data to check the methylation status of gene promoters in the metabolic pathways of interest.
Validate the function of plant genes potentially involved in drought tolerance mechanisms in Arabidopsis, rice or sepia plants through strategies of overexpression or silencing.
Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members.
Márcio Alves Ferreira
Graduated in Biological Sciences with a BA in Genetics from the Federal University of Rio de Janeiro (1991), a PhD in Biological Sciences (Genetics) from the Federal University of Rio de Janeiro/Ghent University (Brazil and Belgium/1997) and a Post-Doctorate in Genetics of plant development at the California Institute of Technology - Caltech (USA/2001). Full Professor at the Department of Genetics at the Institute of Biology at the Federal University of Rio de Janeiro. Has experience in plant genetics, with emphasis on Plant Biotechnology, working mainly on the following topics such as control of gene expression, abiotic stress and functional genomics. Accredited advisor in the postgraduate courses in Genetics (level 7) and in Plant Biotechnology and Bioprocesses (level 6) at UFRJ. He has supervised 25 Master's dissertations, 13 doctoral theses and 10 postdoctoral supervisions. Since 2010, he has been working as a member of the CAPES Biotechnology Area Committee in the postgraduate evaluation.
Plant Molecular Genetics and Biotechnology Laboratory
Bloco A, A2-93 Institute of Biology, Department of Genetics
Universidade Federal do Rio de Janeiro, Cidade Universitária - CEP: 21941-617, Rio de Janeiro, RJ, Brazil.