AL 08 - Soybean Transformation (UFRGS)
Team Leader: Maria Helena B. Zanettini
Laboratory Activities with INCT PlantStress Biotech
Research efforts in our laboratory have focused on prospecting and characterization of genes involved in plant response to biotic and abiotic stresses. Emphasis: Soybean (Glycine max), Jackbean (Canavalia ensiformis) and Solanum nigrum. Approaches: Gene expression analysis; Gene silencing; Overexpression; Subcellular localization of gene products. For genetic transformation, we use mainly particle bombardment, but we are gaining experience on Agrobacterium-mediated transformation. Embryogenic tissues are used as targets for transformation.
• Characterization of soybean genes involved in flood and drought tolerance.
• Overexpression of Jaburetox and Soyuretox, urease-derived peptides, for plant protection against insects, fungi and nematodes.
• Characterization of WRKY soybean genes responsive to water deficit and study of their promoters.
• Expression of a gene that encodes an osmotin, isolated from Solanum nigrum, aiming enhanced drought tolerance in soybean.
Maria Helena Bodanese Zanettini
Universidade Federal do Rio Grande do Sul
Campus do Vale, Bento Gonçalves Avenue 9500, Building 4342212, Postal Code 91501-910, Porto Alegre, RS, Brazil
Phone: +55 51 3008-6725