AL10 - Maize transformation (Embrapa Maize and Sorghum)
Team Leader: Newton Carneiro
Laboratory Activities with INCT PlantStress Biotech
Integrate data from monocotyledon transcriptomes (rice, corn, wheat, Musa and Sorghum) submitted to water deficit, generated by mass sequencing (Illumina - HiSeq) in previous projects.
Validate promoters by transient transformation by biolistics or Agrobacterium rhyzogenes in target plants for in vivo testing of promoters (soybean, cotton and corn).
Validate promoters by stable transformation in target plants for in vivo testing of promoters identified as promising in previous tests (soybean, cotton and corn).
Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members.
Generate GM soybean, GM cotton and GM corn via strategies of overexpression or silencing of plant genes and evaluate the phenotype obtained regarding drought tolerance.
Generate GM soybean, GM cotton and GM corn expressing sequences for silencing of insect genes and evaluate the phenotype obtained regarding the control of Helicoverpa armigera and Spodoptera frugiperda.
Generate GM soybean, GM cotton and GM corn via toxin overexpression strategies and evaluate the phenotype obtained regarding the control of H. armigera and S. frugiperda.
Phenotyping (greenhouse or field) corn, soybean and cotton populations, resulting from crossing with GM events to obtain proof of concept of drought tolerance and/or resistance to S. frugiperda, H. armigera or Meloidogyne spp.