Biotechnological Assets Applied to Drought and Pests in Relevant Crops for Agribusiness
AL 02- Transcriptomics, Epigenetics and Functional Genomics - (UFRGS)
Supervisor: Rogério Margis
Laboratory activities with INCT Collaborators
Research efforts in our laboratory are focused on the prospecting and characterization of genes involved in the response of plants to biotic and abiotic stresses. Emphasis: Soy (Glycine max), Jackbean (Canavalia ensiformis) and Solanum nigrum. Approaches: Analysis of gene expression; Gene silencing; Overexpression; Subcellular location of gene products. For genetic transformation, we mainly use particle bombardment, but we are gaining experience in Agrobacterium-mediated transformation. Embryogenic tissues are used as targets for transformation.
• Response of native Neotropical plants and cultivated plants to abiotic stresses: drought and salinity
• Identification of genes related to plant adaptation and tolerance to abiotic stresses
• Characterization of the interaction network between microRNAs - circRNAs and mRNAs in the regulation of gene expression.
• Study of the mechanisms and quantification of mRNA editing events in chloroplasts in response to stresses.
The Biophysics Department and the UFRGS Biotechnology Center have a laboratory with infrastructure for analysis of gene expression, a Molecular Biology laboratory, a greenhouse and a bioinformatics laboratory. Main equipment available: real-time PCR devices, Step-One 96, ABI-7500 and CFX-384 BioRad, laminar flow cabinets, systems for DNA, RNA and protein electrophoresis, spectrophotometer, fluorimeter,, PCR thermocyclers, analytical and preparative centrifuge. In the Department of Genetics, the Laboratory of Plant Tissue Culture and Genetic Transformation is certified by CQB.
Frank Lino Guzman Escudero - Lattes
João Abreu - Lattes
Nureyev Ferreira Rodrigues - Lattes
Erika Frydrych-Capelari - Lattes
• Natalia Balbinott - Lattes
Fábio Nogueira – IQ - UFRJ
Gilberto Domond – IQ - UFRJ
Felipe dos Santos Maraschin – Dept. Botanica - UFRGS
UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL – UFRGS
Departamento de Biofísica – Sala 206 - LGPP
Av. Bento Gonçalves 9500, Prédio 4342212
Porto Alegre, RS, Brasil
Fone +55 51 3008 6234