Biotechnological Assets Applied to Drought and Pests in Relevant Crops for Agribusiness

AL10 - Maize transformation (Embrapa Maize and Sorghum)

Supervisor: Newton Carneiro

Laboratory activities with INCT                                                                                                 Collaborators

Integrate data from monocotyledon transcriptomes (rice, corn, wheat, Musa and sorghum) submitted to water deficit, generated by mass sequencing (Illumina - HiSeq) in previous projects.

AL5 AL6 AL9

Validate promoters by transient transformation by biolistics or Agrobacterium rhyzogenes in target plants for in vivo testing of promoters (soy, cotton and corn).

AL3 AL8 AL9

Validate promoters by stable transformation in target plants for in vivo testing of promoters identified as promising in previous tests (soybeans, cotton and corn).

AL8 AL9

Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members.

AL4 AL8 AL9

Generate PGMs from soy, cotton and corn via strategies of overexpression or silencing of plant genes validated in ID4 and evaluate the phenotype obtained regarding drought tolerance.

AL9

Generate PGMs from soybean, cotton and corn expressing sequences for silencing of insect genes identified in ID 1 and evaluate the phenotype obtained regarding the control of H. armigera and S. frugiperda.

AL8 AL9

Generate PGMs from soybean, cotton and corn via toxin overexpression strategies and evaluate the phenotype obtained regarding the control of H. armigera and S. frugiperda.

AL8 AL9

Phenotyping (greenhouse or field) maize, soybean and cotton population resulting from crossing GM events identified in IDs 4 and / or 6 to obtain proof of concept of drought tolerance and / or resistance to S. frugiperda, Helicoverpa armigera or Meloidogyne spp.

AL4 AL9 AL11 L13 AL14

Embrapa Recursos Genéticos e Biotecnologia Parque Estação Biológica - PqEB - Av. W5 Norte (final)

Tel: +55 (61) 3448-4705

inctplantstress@gmail.com