Biotechnological Assets Applied to Drought and Pests in Relevant Crops for Agribusiness

AL Collaborator – Embrapa Soybean

Supervisor: Francismar C. Marcelino-Guimarães

Laboratory Description​

In this INCT, Embrapa Soybean’s Plant Biotechnology Laboratory will act in the prospecting of genes involved in parasitism in nematodes, validation of identified genes via transient expression assays (compound soybean plants) and / or virus-induced gene silencing (VIGS), as well as resistance genes and defense mechanisms in soybean. Finally, it will assist in the molecular characterization of transformed soybean plants containing genes previously validated in model plants by AL02 and AL08, aiming at the identification of elite events, useful in the Genetic Improvement Program. We hope that the activities conducted at this research institute can contribute to the development of technologies for the sustainability of the soy production chain, including genotypes adapted to different edaphoclimatic conditions and stresses that affect the crop.

Research lines

  • Genetic and associative mapping of resistance genes to gall nematodes in soybean;

  • Prediction and functional characterization of genes involved in nematode parasitism;

  • Validate the function of plant genes potentially involved in resistance to nematodes via strategies of overexpression or silencing;

  • Validate the function of nematode genes potentially involved in the parasitism mechanisms identified in ID1, via gene silencing strategies (iRNA) in model systems.

 

Infrastructure

Laboratory


The Unit has accredited laboratories and facilities for work in molecular biology, genetic mapping, plant transformation and bioinformatics. The laboratories have two DNA sequencers; two real-time PCR systems; ten thermal cyclers; 2 biobalistic cannons; spectrophotometers; plate centrifuges; in addition to all standard equipment for molecular analysis. Embrapa Soja also has support structures for greenhouses and fields to support laboratory experiments.

Our Team

Post-Doctoral

• André Luiz de Lima Passianotto (Atuação em 2017-2018) - Lattes
• Valeria Stefania Lopes-Caitar (Atuação em 17-2018) - Lattes

• Suellen Mika Hishinuma Silva (Atuação como Doutoranda de 2017-2019/Pós-doutoranda em 2020) - Lattes

Doctoral

• Rafael Bruno Guayato Nomura (Atuação em 2017-2020) - Lattes
• Jean Carlos Alekcevetch (Atuação em 2017) - Lattes

Contact

Centro/Laboratório:
Laboratório de Biotecnologia Vegetal – Embrapa Soja
Campus Rodovia Carlos João Strass, s/nº Acesso Orlando Amaral, Distrito de Warta, Londrina, PR, Brasil
C.Postal 231
Fone +55 43 3371 6000

E-mail: francismar.marcelino@embrapa.br

Link para o site do grupo se tiver: https://www.embrapa.br/en/soja

Analyst Embrapa Soybean

• Danielle Cristina Gregorio da Silva - Lattes

• Marcia Kamogae Kuwahara - Lattes

• Adriana Brombini Dos Santos - Lattes

Post-Doctoral
• André Luiz de Lima Passianotto (Atuação em 2017-2018) - Lattes
• Valeria Stefania Lopes-Caitar (
Atuação em 17-2018) - Lattes

Suellen Mika Hishinuma Silva (Atuação como Doutoranda de 2017-2019/Pós-doutoranda em 2020) - Lattes


Doctoral
• Rafael Bruno Guayato Nomura (Atuação em 2017-2020) - Lattes
• Jean Carlos Alekcevetch (Atuação em 2017) - Lattes

Contact

Centro/Laboratório:
Laboratório de Biotecnologia Vegetal – Embrapa Soja
Campus Rodovia Carlos João Strass, s/nº Acesso Orlando Amaral, Distrito de Warta, Londrina, PR, Brasil
C.Postal 231
Fone +55 43 3371 6000

E-mail: francismar.marcelino@embrapa.br

Link para o site do grupo se tiver: https://www.embrapa.br/en/soja

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Carlos Arrabal Arias

Graduated in Agronomic Engineering (1988) and Master in Genetics and Breeding (1991) from the State University of Londrina and PhD in Agronomy (Genetics and Plant Breeding) from the Luiz de Queiroz School of Agriculture (1995). He is currently a researcher for the Brazilian Agricultural Research Corporation, at the National Agricultural Research Center-Embrapa Soja. He is also a collaborating professor in the postgraduate course in Genetics and Molecular Biology at the State University of Londrina. He is currently a member of the Internal Technical Committee and the Internal Biosafety Committee of Embrapa Soja. Currently he is also the Leader of the National Soy Improvement Project at Embrapa Soja, working mainly on the following themes: soybean cultivars, adaptability and stability, and genetic resistance to insect pests and diseases such as Asian rust.

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Francismar C. Marcelino Guimarães

Francismar Correa Marcelino is a biologist graduated from the Federal University of Viçosa (2000), where he also took a master's and doctorate course in Genetics and Breeding (2006). She did her Postdoctoral (2011) in Molecular Plant Pathology at Iowa State University (US). She is a researcher at Embrapa Soja, working in the Molecular Genetics of Plants area, focusing on plant-pathogen molecular interaction, in the lines of transcriptomy research in response to infection by phytopathogens (P. pachyrhizi and nematodes), effector characterization and functional genomics in soy. It also acts in the identification and validation of SNPs markers via genetic re-sequencing strategies for application in assisted selection for the development of soybean cultivars. She is a professor and advisor at the State University of Londrina in the postgraduate course in Biotechnology and Genetics, and in Bioinformatics at UFTPR. She has experience in the fields of Plant Molecular Genetics, Plant-Pathogen Molecular Interaction, Biochemistry and Biotechnology. She has supervised / co-supervised several students with scientific initiation, doctorate and postdoctoral degrees in the different lines of research she works with.

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Ricardo Vilela Abdelnoor

Researcher at the Brazilian Agricultural Research Corporation (EMBRAPA) since 1994, working in the field of Molecular Genetics and Soy Genomics. Graduated in Agronomy from the Federal University of Viçosa and doctorate in Agronomy (Genetics and Breeding) from the University of Nebraska, United States, in 2004. He currently participates in several research projects and led the multi-institutional projects "Technological Platform for Integrated Management of the Asian Rust of Soy "(FINEP) and" GENOSOJA - National Consortium for Studies of the Soy Genome "(CNPq), in addition to leading projects of Embrapa Macroprogram 2. He is currently an accredited Professor of the Genetics and Molecular Biology program at the State University of Londrina (UEL), having supervised and co-supervised master and doctoral students at the said university. He also serves as co-advisor at the Federal University of Viçosa (UFV), UNESP-Jaboticabal and State University of Maringá (UEM). He coordinated PROCISUR's "Functional Genomics" Platform for five years, and currently serves as a member of the International Soy Genome Consortium and served as an elected member of the Soybean Genetics Committee (2009-2012). In 2009 he was elected to the "Continuing Committee" of the World Soybean Research Conference (World Soybean Research Conference) for the period 2009-2020, and in 2013 he was chosen as President of the "Continuing Committee", for the period 2013 -2020. He was President of the Organizing Committee of the VI Brazilian Soy Congress (CBSoja 2012). He was a member of the National Technical Commission on Biosafety (CTNBIO) from 2012 to 2014. In the management area, he was Executive Secretary of the Internal Technical Committee (CTI) of Embrapa Soja, from 2010 to 2013 and since 2013 he is the Deputy Head of Research & Development Embrapa Soy. Since 2013 he is a member of the Sectorial Chamber of the Soy Productive Chain, linked to the Ministry of Agriculture.

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Waldir Pereira Dias

Graduated in Agronomy from the Federal University of Viçosa (1982), Master in Agronomy (Phytopathology) from the Federal University of Viçosa (1992) and doctorate in Agronomy (Phytopathology) from the Federal University of Lavras (2003). He is currently a researcher at the Brazilian Agricultural Research Corporation. He has experience in the field of Agronomy, with an emphasis on Phytopathology, acting mainly on the following themes: development of soybean cultivars with resistance to nematodes, with emphasis on gall nematodes (Meloidogyne spp.) And the soy cyst nematode (Heterodera glycines).

Embrapa Recursos Genéticos e Biotecnologia Parque Estação Biológica - PqEB - Av. W5 Norte (final)

Tel: +55 (61) 3448-4705

inctplantstress@gmail.com