Prospection of genes and molecules involved in resistance/tolerance to specific and combined stresses in native plants and in resistant/tolerant contrasting genotypes, through elucidation of the stress mechanisms and signaling pathways;
Prospection of genes and molecules involved on defense and parasitism of the target pests;
Search for promoters responsive to pests and water deficit and small RNAs involved in regulation of target genes;
Apply different strategies (gene/molecules overexpression or silencing) for biological functional validation in model systems.
Developing new nanoencapsulated insecticides/nematicides from validated molecules;
Generation of biotechnological products – concept proof in target plants (maize, soybean and cotton);
Evaluation of the target plant in the field.
The actives generated will be applied in the development of biotechnology products such as bioinsecticides and multi resistant/tolerant plants genetically modified, for the studied stresses, of soybean, maize and cotton. In addition, the actives generated in this project, the understanding of molecular and physiological factors related to drought tolerance and interaction with pests and others environmental stresses will contribute to prevent, unveil and adapt to climatic changes.
In parallel with its scientific agenda, the INCT group formed by researchers from five Embrapa units and professors from five Federal Universities (UnB, UFRJ, UFRGS, UFC and UFPel), and collaborators from public and private sectors, propose to focus their efforts in training, in Brazil and exterior, students and professionals from related areas, mainly biotechnology, genomics and bioinformatics. The actions will also strengthen the joint action and internationalization of undergraduate and graduate courses involved with INCT, as well as increasing the visibility and international insertion of its teams.
For the future, the INCT network can be used not only for generation of active biotechnologies applied to drought and pests in soybean, cotton and maize but also for others agronomic important traits (seed and fruit quality, nutrition value increase and others) and for other important crops for the Brazilian agribusiness.
Investigations regarding biological systems focusing on elucidation of the plant stresses mechanisms and signaling pathways are important to develop resistant genotypes in a climate change scenario. Pathogens attack and abiotic stresses such as water deficit are critical during plant development and the identification of genes and molecules which regulate resistance responses are important to develop genotypes able to dealing with these stresses.
In association with abiotic stresses, plants also need to defend themselves against pathogen attacks such as fungus, bacteria and nematodes. Pest insects comprehend the most critical factor affecting productivity losses in Brazilian agribusiness. Losses in soybean and cotton caused by nematodes and losses caused by the caterpillars H. armigera and S. frugiperda which affect maize production, comprehend the most important phytosanitary problem in competitiveness for commodities exportation.
Facing these scenario cases of genotype resistance breakdown, environmental association of biotic and abiotic stresses, fewer characterized and introgressed genes and higher complexity of plant responses, have been reported. These reports demonstrate the importance of the research towards the identification of genes and molecules involved in resistance responses against pests and water deficit.
In this context, the “INCT Biotechnology applied to plant stresses” proposes to integrate diverse Brazilian research groups and international partners, which are expert in plant physiology, transcriptomic, epigenetic, proteomic and bioinformatics analyses and functional genomics. The integrative research group aims to develop a multidisciplinary and multi institutional network with national and international excellence to generate biotechnologies applied to maize, soybean and cotton aiming tolerance to water deficit and pest control (Meloidogyne spp, H. armigera and S. frugiperda). This proposal includes prospection, isolation, characterization and functional validation of genes and molecules involved in resistance against pathogens and water deficit tolerance. INCT action comprises:
About the project