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Learn more about AL02, its main goals within the project and meet the laboratory's work team. AL 02 - Transcriptomics, Epigenetics and Functional Genomics Laboratory Activities - PlantStress Biotech INCT Sequencing the genotype plant native species from Brazil (pitangueira). Sequencing of transcriptomes associated with the response to drought of native species in Brazil (wild peanuts, pitangueira, Clúsia, and cashew) by large-scale sequencing. Identify and select in silico candidate genes related to drought tolerance of native species (wild peanuts, pitangueira, Clúsia, and cashew). Identify InDels and SNPs in candidate genes of native species associated with the drought response (wild peanuts, pitangueira, and cashew). Validate in vitro the expression profile of candidate genes for drought tolerance obtained in native species of Brazil (wild peanuts, pitangueira, Clúsia, and cashew). Sequencing using the Illumina platform a fraction of small RNAs, and their target mRNAs, and circular RNAs of plants (Arachis, Musa, soybean, pitangueira, and cashew tree) submitted to biotic and/or abiotic stresses. Analyze the sequencing data to check the methylation status of gene promoters in the metabolic pathways of interest. Validate the function of plant genes potentially involved in drought tolerance mechanisms in Arabidopsis, rice or sepia plants through strategies of overexpression or silencing. Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Laboratory Description Research efforts in our laboratory are focused on the prospecting and characterization of genes involved in the response of plants to biotic and abiotic stresses. Emphasis: Soybean (Glycine max ), Jackbean (Canavalia ensiformis ) and Solanum nigrum . Approaches: Analysis of gene expression; Gene silencing; Overexpression; Subcellular location of gene products. For genetic transformation, we mainly use particle bombardment, but we are gaining experience in Agrobacterium -mediated transformation. Embryogenic tissues are used as targets for transformation. Research Lines Response of native Neotropical plants and cultivated plants to abiotic stresses: drought and salinity. Identification of genes related to plant adaptation and tolerance to abiotic stresses. Characterization of the interaction network between microRNAs - circRNAs and mRNAs in the regulation of gene expression. Study of the mechanisms and quantification of mRNA editing events in chloroplasts in response to stresses. Our Team Rogerio Margis Team Leader Rogerio Margis completed his doctorate at the Institut de Biologie Moleculaire des Plantes, IBMP of CNRS, at the Université Louis Pasteur of Strasbourg I, France, in 1993. In 2002 he did a post-doctorate related to the processes of RNA interference and production of microRNAs in plants in the CSIRO's Plant Industry in Canberra, Australia. He is currently a CNPq researcher and Full Professor in the Department of Biophysics and Biotechnology Center at the Federal University of Rio Grande do Sul. Full member of the Brazilian Academy of Sciences since 2016. From 1994 to 2003 he was an adjunct professor at UFRJ. At UFRGS, he works as a permanent core researcher and advisor in the graduate programs in Genetics and Molecular Biology (PPGBM) and Cell and Molecular Biology (PPGBCM). Currently participates in research projects related to the themes: RNA interference processes, small RNAs and non-coding RNAs in rice, soybean and native Neotropical species; action of cysteine proteases and their inhibitors; proteins related to abiotic stresses (cold, drought and metals) and oxidative stress (APx and GPx). He works in the areas of Genetics and Biochemistry, with an emphasis on Molecular Biology: regulation of gene expression and molecular markers. In his professional activities, he interacted with more than a hundred national and international collaborators in projects and co-authorship of scientific works. Márcia Pinheiro Margis Graduated in Biological Sciences at the State University of Rio de Janeiro (1983), Master's degree in Biochemistry from the Federal University of Rio de Janeiro (1987) and doctorate in Biologie Moleculaire Des Plantes - Universite de Strasbourg I - France (1993). Full Professor at the Department of Genetics at the Federal University of Rio Grande do Sul. She is a full member of the Brazilian Academy of Sciences. Full member of the CNPq Genetics Advisory Committee (CA-GE) between July 2011 and June 2014. She was a member of the board of the Brazilian Society of Genetics (SBG), having been first treasurer, first secretary, vice president, and president (2016 to 2018). Member of CTNBio between March 2012 and February 2014. Coordinator of the Graduate Programs in Genetics at the Federal University of Rio de Janeiro (2000-2001) and the Federal University of Rio de Grande do Sul (2011 to 2015). Editor of the journal Genetics and Molecular Biology. President of the International Genetics Federation (since September 2018). She has experience in the field of Genetics, with an emphasis on Plant Genetics, working mainly on the following topics: defense responses of plants against abiotic stresses, antioxidant metabolism enzymes and functional plant genomics. Contact Rogerio Margis Universidade Federal do Rio Grande do Sul - Department of Biophysics - Room 206 - LGPP Campus do Vale, Bento Gonçalves Avenue 9500, Building 4342212, Postal Code 91501-910, Porto Alegre, RS, Brazil E-mail: rogerio.margis@ufrgs.br Phone number: +55 51 3008-6234

Learn more about AL07, its main goals within the project and meet the laboratory's work team. AL 07 - Insects and Nematodes: Breeding and Bioassays Laboratory Activities - PlantStress Biotech INCT Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Leonardo Lima Pepino de Macedo Team Leader Dr. Leonardo is undergraduated in Biological Sciences from the Federal University of Rio Grande do Norte (2005). He holds a master's degree in Biochemistry from the Federal University of Rio Grande do Norte (2007), and a doctorate's degree in Genomic Science and Biotechnology from the Catholic University of Brasília (2012). He has experience in Biochemistry and Molecular Biology, with expertise in the following topics: cloning and expression of proteins in heterologous systems; bioprospecting of proteins with entomotoxic activity (vicillins, lectins, proteinase inhibitors and Cry toxins) aiming at the control of dipterous, lepidopteran and coleopteran insects; and development of gene silencing strategies via interfering RNA (RNAi) for the control of insect pests. Contact Leonardo Lima Pepino de Macedo EMBRAPA Genetic Resources and Biotechnology W5 Norte Avenue (end) - P.O. Box 02372 - Postal Code 70770-917 - Brasília, DF - Brazil E-mail: leonardo.lima@embrapa.br Phone number:+55 61 3448-4705

Learn more about AL01, its main goals within the project and meet the laboratory's work team. AL 01 - Genomics and Proteomics Laboratory Activities - PlantStress Biotech INCT Integration data from monocotyledon transcriptome (rice, corn, wheat, Musa and sorghum) submitted to water deficit, generated by mass sequencing (Illumina - HiSeq) in previous projects. Integration of transcriptome data from drought-tolerant genotypes of Musa spp., Arachis spp. and cowpea subjected to water deficit combined with biotic stress (Meloidogyne spp. or Mycosphaerella ) in bioassays. Integration and sequencing of transcripts of fungus-tolerant genotypes in environments subjected to multiple stresses (Musa X Mycosphaerella musicola and Fusarium oxysporun ). Validation of key genes expression in the metabolic pathways of plants' response to combined stresses (biotic-biotic; biotic-biotic; abiotic-biotic) by qRT-PCR. Small RNAs, mRNAs and circular plant RNAs sequencing from Arachis , Musa , soybean, pitangueira, and cashew, previously subjected to biotic and/or abiotic stresses, using the Illumina platform. Validation of plant genes function potentially involved in drought tolerance mechanisms in Arabidopsis , rice or Setaria plants by overexpression or silencing strategies. Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Laboratory Description The Genomics and Proteomics Laboratory studies the prospection and identification of new genes, regulatory sequences and molecules involved with the resistance/tolerance responses of plants to biotic and abiotic stresses, aiming at the elucidation of the molecular mechanisms of plants that occur in response to different stresses, combined or not. The research group also performs fucntion validation of these assets in model plants. Research Lines Prospecting genes/molecules of interest for pest and drought control in wild germplasm of Musa spp. Prospecting target molecules in phytoparasitic nematodes (Meloidogyne spp.) and phytopathogenic fungi (Pseudocercospora spp.) for pest control. Prospecting for small RNAs in plant genotypes that are resistant/tolerant to pests and drought that may be involved in responding to these stresses. Prospecting for genes/molecules that are efficient in pest control and tolerance to water deficit simultaneously (cross-stress). Validation of prospective innovation assets by their overexpression or gene silencing in model plants for analysis and validation of their function. Robert Neil Gerard Miller Team Leader Graduated in Biological Sciences - Manchester Metropolitan University, UK (1990), Master in Plant Protection - University Of Bath, UK (1991) and PhD in Molecular Biology and Phytopathology - University of Reading, UK (1995). He is currently Associate Professor I at the University of Brasilia (Campus Darcy Ribeiro, Department of Cellular Biology), supervising the Graduate Programs in Molecular Biology, Phytopathology and Microbial Biology. He worked between 2014 and 2016 as Coordinator of the Graduate Program in Molecular Biology (CAPES concept 6). He works as editor for the journals Annals of Botany and Tropical Plant Pathology, he is coordinator of national and international projects, mainly in the following themes: functional genomics of plants and microorganisms; search for genes for resistance to biotic stress in plants; and characterization of phytopathogenic and mycotoxigenic fungi. Contact Robert Neil Gerard Miller Microbiology Laboratory: Plant-Prague Interaction, Bloco I-1-35/8, Institute of Biological Sciences, Department of Cell Biology, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Asa Norte, Postal Code 70910-900, Brasília, DF, Brazil.

Learn more about AL03, its main goals within the project and meet the laboratory's work team. AL 03 - Molecular Genetics Laboratory Activities - PlantStress Biotech INCT Integration of the transcriptome and sequencing of the model plant (Setaria) submitted to drought and high carbon dioxide content. Validate the expression of key genes in the metabolic pathways of plants response to combined stresses (biotic-biotic; biotic-biotic; abiotic-biotic) by qRT-PCR. Identify in silico promoters responsive to biotic and abiotic stress (drought and phytonematodes), with activity in different organs (root and leaf). Set up a vector bank with promoters directing the expression of reporter genes (GUS/GFP) in monocots and dicots. Validate promoters by transient transformation by biolistics or Agrobacterium rhyzogenes in target plants for in vivo testing of promoters (soybean, cotton and corn). Analyze the sequencing data to check the methylation status of gene promoters in the metabolic pathways of interest. Validate the function of plant genes potentially involved in drought tolerance mechanisms in Arabidopsis , rice or sepia plants through strategies of overexpression or silencing. Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Márcio Alves Ferreira Team Leader Graduated in Biological Sciences with a BA in Genetics from the Federal University of Rio de Janeiro (1991), a PhD in Biological Sciences (Genetics) from the Federal University of Rio de Janeiro/Ghent University (Brazil and Belgium/1997) and a Post-Doctorate in Genetics of plant development at the California Institute of Technology - Caltech (USA/2001). Full Professor at the Department of Genetics at the Institute of Biology at the Federal University of Rio de Janeiro. Has experience in plant genetics, with emphasis on Plant Biotechnology, working mainly on the following topics such as control of gene expression, abiotic stress and functional genomics. Accredited advisor in the postgraduate courses in Genetics (level 7) and in Plant Biotechnology and Bioprocesses (level 6) at UFRJ. He has supervised 25 Master's dissertations, 13 doctoral theses and 10 postdoctoral supervisions. Since 2010, he has been working as a member of the CAPES Biotechnology Area Committee in the postgraduate evaluation. Contact Marcio Alves-Ferreira Plant Molecular Genetics and Biotechnology Laboratory Bloco A, A2-93 Institute of Biology, Department of Genetics Universidade Federal do Rio de Janeiro, Cidade Universitária - CEP: 21941-617, Rio de Janeiro, RJ, Brazil.

Find out more about the project, the specific objectives and the institutions involved. Acerca de About the Project Biological systems research focusing on elucidating plant stress mechanisms and signaling pathways is important for developing resistant cultivars in a climate change scenario. Pathogen attacks and abiotic stresses such as water deficit are essential during plant development, and the identification of genes and molecules, which regulate resistance responses, are critical to develop crop cultivars capable of doing in the face of these stresses. In association with abiotic stresses, plants must also defend themselves against pest’s attacks, including fungi, bacteria, insect pests, and nematodes. Insect-pests comprise the most serious factor affecting productivity losses in the Brazilian agribusiness. Losses in soybean, cotton, and corn caused by the caterpillars Helicoverpa armigera , Spodoptera frugiperda , and phytonematodes constitute the most critical phytosanitary problem in terms of competitiveness for the commodities production and exportation. Faced with scenario cases involving plant resistance breakdown, environmental association of biotic and abiotic stresses, fewer characterized and introgressed genes, and higher complexity of plant responses have been reported. All these reports demonstrate the importance of the research towards the identification of genes/molecules involved in pest resistance and water deficit responses. In this context, the National Institute of Science and Technology, PlantStress Biotech INCT, integrates various Brazilian research groups and international partners, experts in plant physiology, transcriptomic, epigenetic, proteomic, bioinformatic and functional genomics analyses. The integrative research group represents a multidisciplinary and multi-institutional network with national and international excellence to generate innovative biotechnologies applied to corn, soybean and cotton focused on the tolerance to deficit hydric and pest control (Meloidogyne spp, H. armigera and S. frugiperda ). The project includes bioprospection, isolation, characterization, and functional validation of genes/molecules involved in plant pest resistance and drought tolerance. The PlantStress Biotech INCT action comprises: Prospection for genes and molecules involved in resistance/tolerance to specific and combined stresses in native plants and resistant/tolerant contrasting genotypes, through the elucidation of the stress mechanisms and signaling pathways; Prospection for genes and molecules involved in the defense and parasitism of target pests; Search for promoters responsive to pests and water deficit, and small RNAs involved in the regulation of target genes; Use different strategies (gene/molecules overexpression or gene silencing) for biological functional validation in model systems; Developing of new nanoencapsulated insecticides/nematicides from validated molecules; Developing of biotechnological products (concept proof) in GM crop plants (cotton, soybean, and maize); Evaluation of developed GM crop plants in simulated field experiments. The biotech assets generated will be applied to the development of biotechnology products, including nanobioinsecticides and genetically modified crops (soybean, maize, and cotton) against biotic and abiotic stresses. In addition to the biotech assets obtained and characterized in this project, an understanding of molecular and physiological factors related to drought tolerance and their interaction with pests and other environmental stresses will contribute to prevent, unravel and adapt to climatic changes. Alongside its scientific agenda, the PlantStress Biotech INCT group, formed by researchers from five Embrapa units and five Federal Universities (UnB, UFRJ, UFRGS, UFC, and UFPel), and collaborators from the public and private sectors, concentrate their efforts on training students and professionals in different and update approaches involving biotechnology, genomics, and bioinformatics. Actions will also strengthen joint action and the internationalization of undergraduate and graduate training engaged in PlantStress Biotech INCT research, and to increase the visibility and international insertion of its teams. The PlantStress Biotech INCT network focuses not only on the generation of biotech assets applied to drought and pests in soybean, cotton, and maize but also on other important agronomic traits (seed and fruit quality, an increase of biomass and nutritional value, among other) and in other crops important for Brazilian agribusiness.

Embrapa Genetic Resources and Biotechnology. Parque Estação Biológica - PqEB - W5 Norte Avenue (end) P.O. Box 02372 - Brasília, DF - Brazil - Postal Code 70770-917. Phone number: +55 61 3448-4902 Contact Coordinator: Maria Fatima Grossi-de-Sa Embrapa Genetic Resources and Biotechnology Parque Estação Biológica - PqEB - W5 Norte Avenue (end) P.O. Box 02372 - Brasília, DF - Brazil - Postal Code 70770-917 Phone number: +55 61 3448-4902 E-mail: fatima.grossi@embrapa.br /inctplantstresssite@gmail.com You can also contact us by filling this contact form: First Name Last Name Email Phone Subject Message Submit Thanks for submitting!

Learn more about AL13, its main goals within the project and meet the laboratory's work team. AL 13 - Field Phenotyping - Public Sector Laboratory Activities - PlantStress Biotech INCT Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Phenotyping (greenhouse or field) cotton population resulting from crossing with GM events to obtain proof of concept of drought tolerance and/or resistance to Spodoptera frugiperda , Helicoverpa armigera or Meloidogyne spp. Jaime Vasconcelos Cavalcanti Team Leader He has a degree in Agronomy from the Federal Rural University of Pernambuco (1987), master's degree in Agronomy (Genetics and Plant Improvement) from the Federal University of Lavras (1997) and a PhD in Molecular Biology - University of Reading, England (2004). He is currently a researcher at the Brazilian Agricultural Research Corporation, acting as coordinator of the Platform Project for the development of transgenic cotton plants resistant to the boll weevil at Embrapa Algodão. He is a permanent professor of postgraduate courses in plant genetic improvement at the Federal Rural University of Pernambuco (UFRPE) and agricultural sciences at the State University of Paraíba (UEPB), teaching, in both, the discipline of Quantitative Genetics. He has experience in the area of Agronomy, with an emphasis on plant genetic improvement, mainly on the following topics: genetic improvement of white and colored cotton, quantitative genetics, transgenics, molecular genetics and genomic statistics. Carlos Alberto D. da Silva Agronomist from the Faculty of Agronomy and Zootechnics Manoel Carlos Gonçalves (1986) holds a master's degree in Agronomy (Plant Protection) from the Universidade Estadual Paulista Júlio de Mesquita Filho (1992), a doctorate in Entomology from the Federal University of Viçosa (2006) and specialization in management by Fundação Dom Cabral (2009). Approved in the public competition of the Faculty of Agronomy of Vale do São Francisco (FAMESF) of the State University of Bahia (UNEB) on March 21, 1994, he served as assistant professor teaching plant health for a year, when he left to take on the position of researcher at Embrapa. He has been a researcher at Embrapa Algodão since December 22, 1994 to the present day. At this research institution he served as Deputy Head of Research and Development from March 2008 to July 2013. Invited to work as a professor of the postgraduate course in Agricultural Sciences at the State University of Paraíba (UEPB) in August 2010 teaches agricultural entomology within the discipline of plant health of bioenergetic plants. He coordinated, participated and participates in several research projects related to the integrated management of cotton pests in partnership with public and private companies in Brazil, in addition to collaborating with international cooperation projects with African countries. He has experience in the area of Agronomy, with an emphasis on Agricultural Entomology, working mainly with biological control and plant resistance to insects. Jose Ednilson Miranda Agronomist from the State University of Londrina (1995), Master's degree in Zootechnics (Animal Production Concentration Area) from the São Paulo State University Júlio de Mesquita Filho (1998) and PhD in Agronomy (Agricultural Entomology Concentration Area) from the Paulista State University Júlio de Mesquita Son (2001). Researcher at the Brazilian Agricultural Research Corporation since July 2002. He has experience in the area of Agricultural Entomology, working on the topics of integrated cotton pest management, biological control, insecticidal plants. Contact Jaime Vasconcelos Cavalcanti EMBRAPA Cotton Rua Oswaldo Cruz, n° 1.143, Bairro Centenário, CEP: 58428-095, Campina Grande, PB E-mail: jaime.cavalcanti@embrapa.br Phone:+55 83 3182 4300

Learn more about AL15, its main goals within the project and meet the laboratory's work team. AL 16 - Biotechnological Applications of Microorganisms Laboratory Description - PlantStress Biotech INCT Virus:plant interaction and the identification of viruses associated with cotton diseases through sequencing and characterization of new viruses, development of molecular diagnostic tests, transcriptome and miRNA profile during viral infection. The main lines are the characterization of the molecular mechanism of resistance to CBD; the use of fungal molecules as inducers of protection against viral diseases; and the expression of SARS-CoV-2 antigens in plants. Research Lines Cell Biology, Biochemistry and Genetics of Microorganisms Biotechnological Applications of Microorganisms and their Products Microorganisms/Virus-Host Interactions Our Team Maite Vaslin de Freitas Silva Team Leader Associate Professor IV, member of PPG-Micro and PPG-PBV at UFRJ. Director of the Brazilian Society of Virology. Master's and PhD in Genetics from UFRJ in molecular biology of plants. Postdoc at IB UFRJ studying RNAi in antiviral defense. Visiting professor IB UFRJ 199-2001 and permanent professor at IMPG since 2002. I coordinate the Plant Molecular Virology Laboratory, whose lines are: the study of the virus: plant interaction and the identification of viruses associated with cotton diseases through sequencing and characterization of new viruses, development of molecular diagnostic tests, transcriptome and miRNA profile during viral infection. Today the main lines are the characterization of the molecular mechanism of resistance to CBD; the use of fungal molecules as inducers of protection against viral diseases; and the expression of SARS-CoV-2 antigens in plants. The group has 2 patents filed and 1 granted and is encouraging the birth of startups based on the research developed. Contact Maite Vaslin de Freitas Silva Federal University of Rio de Janeiro - UFRJ Department of Virology – Paulo de Góes Institute of Microbiology – UFRJ E-mail: maite@micro.ufrj.br Phone:+55 21 2560 8344

Learn more about AL05, its main goals within the project and meet the laboratory's work team. AL 05 - Plant-Pest Interaction Laboratory Activities - PlantStress Biotech INCT Sequencing transcriptomes associated with the response to drought of native species in Brazil (wild peanuts, pitangueira, Clúsia; and cashew) by large-scale sequencing. Identify and select in silico candidate genes related to drought tolerance of native species (wild peanuts, pitangueira, Clúsia, and cashew). Identify InDels and SNPs in candidate genes of native species associated with the drought response (wild peanuts, pitangueira, and cashew). Validate in vitro the expression profile of candidate genes for tolerance to drought obtained from native species in Brazil (wild peanuts, pitangueira, Clúsia, and cashew). Select vital genes/molecules for gall nematodes (Meloidogyne spp.) by analyzing their genome. Select potential genes involved in resistance from contrasting genotypes (peanuts, soybean, rice, cotton and coffee). Integrate legume transcriptome data (beans, soybean, and peanuts) submitted to water deficit, generated by mass sequencing (Illumina – HiSeq) in previous projects. Integrate transcriptome data from resistant genotypes (beans, soybean, rice, coffee, and peanuts) infected by nematodes, generated by mass sequencing (Illumina – HiSeq) in previous projects. Sequencing and integrating transcriptome of drought tolerant genotypes of Musa spp., Arachis spp., and cowpea subjected to water deficit combined with biotic stress (Meloidogyne spp. or Mycosphaerella ) in bioassays. Validate the expression of key genes in the metabolic pathways of plants' response to combined stresses (biotic-biotic; biotic-biotic; abiotic-biotic) by qRT-PCR. Sequencing on the Illumina platform a fraction of small RNAs, and their target mRNAs, and circular RNAs of plants (Arachis ; Musa ; soybean; pitangueira; cashew tree) subjected to biotic and/or abiotic stresses. Analyze sequencing data to check methylation status of gene promoters in the metabolic pathways of interest. Validate the function of plant genes potentially involved in the mechanisms of drought tolerance in Arabidopsis , rice or sepia plants via overexpression or silencing strategies. Validate the function of plant genes potentially involved in nematode resistance mechanisms via overexpression or silencing strategies. Validate the function of nematode genes potentially involved in the parasitism mechanisms, via gene silencing strategies in model systems. Assess the potential of biotechnological assets generated for the purpose of intellectual protection. Patent the use of genes and gene elements validated during the project Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Laboratory Description The Plant-Pest Interaction Laboratory conducts studies on the interaction of plants with biotic and abiotic stresses, aiming at elucidating the changes in the cellular, biochemical, physiological and molecular machinery of plants, which occur in response to different stresses, combined or not. In addition to prospecting and identifying genes, regulatory sequences and molecules involved in plant tolerance/resistance responses to one or more stresses, research group performs the validation of function of these assets in model plants and methods are developed and improved for the validation of these assets in target plants, which will enable the development of cultivars more adapted to different environmental conditions. Research Lines Prospecting for genes/molecules or peptides of interest for the control of drought and pests in wild peanut germplasm (Arachis spp.). Prospecting of target molecules in phytoparasitic nematodes (Meloidogyne spp.) for pest control. Prospecting for small RNAs in plant genotypes that are resistant/tolerant to pests and drought that may be involved in these stresses. Prospecting for molecules and peptides that are efficient in controlling pests and tolerating water deficit simultaneously (cross-stress). Vallidation of assets due to their overexpression or gene silencing in model plants for analysis and validation of their function. Our Team Patrícia Messemberg Guimarães Team Leader Graduated in Agronomy from the University of Brasília (1985), master's degree in Phytopathology from the University of Brasília (1987) and doctorate in Molecular Biology - University of London (1997). She did post-doctorate in plant genomics at CIRAD (France) in 2006. She is currently a collaborator at the University of Brasilia (Unb) and Catholic University of Brasilia (UCB) and researcher at the Brazilian Agricultural Research Corporation since 1989. The main research areas include Biochemistry, Molecular Biology, Plant-pathogen interaction, and works mainly on the following themes: structural and functional genomics of legumes, Arachis genomics, plant resistance, plant tolerance to water stress, genetic maps, and molecular characterization of plants. She is coordinator of several national and international projects in the field of genetics and genomics of legumes and plant-pest interaction. Ana Cristina Miranda Brasileiro Graduated in Forestry Engineering from the University of Brasília (1986), Master in Molecular and Plant Cell Biology - Universite de Paris XI (Paris-Sud) (1988) and PhD in Molecular and Plant Cell Biology - Universite de Paris XI (Paris-Sud ) (1992). He is currently a researcher at the Brazilian Agricultural Research Corporation. From 2002 to 2006 she worked as a researcher at Labex-Europa at Cirad (France). He has experience in the field of Genetics, with an emphasis on Plant Genetics, working mainly on the following themes: genetic transformation, plant biotechnology, Agrobacterium biology, gene expression and genomics. Since 2006, she has been coordinator in Brazil of the International Consortium in Advanced Biology (CIBA), an initiative of Agropolis (France) and Embrapa (Brazil), whose objective is to create and consolidate an efficient strategy of international scientific and technical cooperation, to study and explore the diversity in genetic resources of plants and to identify important genes and characteristics essential for genetic improvement programs in Tropical and Mediterranean agriculture. From 2008 to 2014 he participated as an external member of the Scientific Council of the Department of Biological Systems (BIOS) of Cirad/France and since 2016 he has been an external member of the Scientific and Strategic Council of Cirad/France. Ana Claudia Guerra de Araújo Researcher Ana Claudia Guerra de Araujo has a degree in Biology from the University of Brasília (1987), a PhD in Biological Sciences (Biophysics) from the Federal University of Rio de Janeiro (1994). Since 1994 she is a Researcher at Embrapa, at Cenargen, where she has been working with the interface between cellular and plant molecular biology at the Microscopy Laboratory. He has a post-doctorate in Australia (CSIRO, 2001) where he worked with molecular and cellular techniques in plant reproduction and in England (University of Leicester, 2011), where he worked with plant molecular cytogenetics using the microscopy tool. At Embrapa, he develops research in the area of plant reproduction, through studies of morphology, cell biology and ultrastructure of developmental biology, involving techniques of cytochemistry, immunocytochemistry, cytogenetics, in situ hybridization associated with microscopy. It is also involved in studies on the plant-pathogen interaction, responses to biotic and abiotic stresses and quality in Arachis, determining factors for the success of a productive and sustainable agriculture. André Southernman Teixeira Irsigler Graduated in Biological Sciences (2000), Master (2002) and Doctorate (2007) in Genetics and Breeding from Universidade Federal de Viçosa, with a sandwich period at North Carolina State University, and Post-doctorate at Florida State University. He is a researcher at Embrapa Genetic Resources and Biotechnology, and is involved in the areas of regulation of gene expression and developmental biology. Contact Patricia Messenberg Guimarães EMBRAPA Genetic Resources and Biotechnology W5 Norte Avenue (end) - P.O. Box 02372 - Postal Code 70770-917 - Brasília, DF - Brazil E-mail: patricia.guimaraes@embrapa.br Phone:+55 61 3448-4787

Get to know all the 15 associated laboratories (AL) who develop INCT PlantStress Biotech projects. Associated Laboratories (ALs) AL 01- Genomics and Proteomics Team Leader: Robert N. G. Miller (UnB) Partner Institutions: UnB, Embrapa Cenargen AL 02 - Transcriptomics, Epigenetics and Functional Genomics Team Leader: Rogério Margis (UFRGS) Partner Institutions: UFRGS, UFRJ, Embrapa Cenargen AL 03 - Molecular Genetics Team Leader: Márcio Alves-Ferreira (UFRJ) Partner Institutions: UFRJ, Embrapa Cenargen AL 04 - Molecular Physiology Team Leader: Joaquim A. G. Silveira (UFC) Partner Institutions: UFC, UFPel, Embrapa Clima Temperado AL 05 - Plant-Pest Interaction Team Leader: Patrícia Messenberg Guimarães (Embrapa Cenargen) Partner Institutions: Embrapa Cenargen, Embrapa Soja, Embrapa Cerrados, Embrapa Arroz e Feijão, Embrapa Milho e Sorgo AL 06 - Bioinformatics Roberto Coiti Togawa (Embrapa Cenargen) Partner Institutions: Embrapa Cenargen, UnB, UFPel AL 07 - Insects-Nematodes: Creation and Bioassays Team Leader: Leonardo Pepino (Embrapa Cenargen) Partner Institutions: Embrapa Cenargen, Embrapa Soja, Embrapa Milho e Sorgo AL 08 - Plant Transformation - Soybean Team Leader: Maria Helena Zanettini (UFRGS) Partner Institutions: UFRGS AL 09 - Plant Transformation - Cotton Team Leader: Maria Fátima Grossi-de-Sa (Embrapa Cenargen) Partner Institutions: Embrapa Cenargen AL 10 - Plant Transformation - Corn Team Leader: Newton Carneiro (Embrapa Milho e Sorgo) Partner Institutions: Embrapa Milho e Sorgo AL 11 - Biometrics Team Leader: Antônio C. de Oliveira (UFPel) Partner Institutions: UFPel AL 12 - Evaluation of Environmental Risks Carmen Pires (Embrapa Cenargen) Angharad Gatehouse (Newcastle University) Partner Institution: Embrapa Cenargen e Newcastle University AL 13 - Field Phenotyping - Public Sector Team Leader: Jaime Cavalcanti (Embrapa Algodão) Partner Institutions: Embrapa Soja, Embrapa Milho e Sorgo, Embrapa Algodão, Embrapa Clima Temperado AL 14 - Field Phenotyping - Private Sector Team Leader: Rafael Galbieri (IMAmt) Partner Institutions: IMAmt - Instituto Matogrossense do Algodão AL 15 - Molecular Plant-Pathogen Interaction Team Leader: Francismar C. Marcelino Guimarães (Embrapa Soja) Partner Institutions: Embrapa Soja AL 16 - Biotechnological Applications of Microorganisms Team Leader: Maite Vaslin de Freitas Silva (UFRJ) Partner Institutions: Federal University of Rio de Janeiro

Explore publications of scientific articles, patents, Thesis and Dissertations developed by INCT PlantStress Biotech. Publications Scientific Papers Patents Dissertations and Thesis

Learn more about AL12, its main goals within the project and meet the laboratory's work team. AL 12 - Colaboration Lab Laboratory Activities - PlantStress Biotech INCT Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Carmen S. Soares Pires Team Leader Dr. Carmen is undergraduated in Biology from the Federal University of Viçosa (1984), holds a master's degree in Entomology from the same university and a Ph.D in Biology fomr the Northern Arizona University (1998). She has been a researcher at Embrapa Genetic Resources and Biotechnology since 1989. She has experience in Ecology, with amphasis on Insect Ecology, working mainly o the following topics: population dynamics, plant-insect pest-natural enemies interaction, conservative biological control and pollination in agroecosystems. Since 2003, she has been involved in environmental risk analysis projects of stressors on non-target organisms (predatory insects, pollinators and non-target herbivores). Angharad MR Gatehouse Team Leader Prof Angharad Gatehouse has the Chair of Invertebrate Molecular Biology at Newcastle University and is Director of Expertise for BioEconomy. Her research focuses on the molecular and biochemical bases of plant-pest interactions with a view to developing novel strategies for crop protection. Her group was one of the first to produce insect-resistant transgenic crops. More recently her group has been using functional genomics to better understand the molecular responses of crops to biotic stress (pathogens and insect pests) for the development of molecular markers. In collaboration with Durham University, her group are actively involved in developing novel biopesticides including those based on RNAi. In addition to their efficacy, the group is also involved in the biosafety of these strategies, notably in terms of their potential effects on beneficial insects such as pollinators and natural enemies. She has published extensively in the field, including in discovery journals (Nature, PNAS). Contact Carmen S. Soares Pires Embrapa - Recursos Genéticos e Biotecnologia Parque Estação Biológica - PqEB, s/n, Brasília - DF carmen.pires@embrapa.br +55 61 3448-4433 Angharad MR Gatehouse School of Natural and Environmental Sciences - Newcastle University, Ridley Building. a.m.r.gatehouse@ncl.ac.uk +44 (0) 191 208 8838