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< Back INCT PlantStress Biotech research highlighted at a student meeting The research carried out at the INCT PlantStress Biotech was highlighted during the XXVII Student Talent Meeting, held at Embrapa Recursos Genéticos e Biotecnologia (Cenargen), in Brasília, between August 28 and 30. Sara Vitorino da Rocha Lemes and Bruna Medeiros Pereira received the award for best work in the Biotechnology category, at undergraduate and postgraduate level, respectively. Sara Rocha is an undergraduate student in Biotechnology at the University of Brasilia (UnB) and a Scientific Initiation scholarship holder under the PIBIC/CNPq/Embrapa program, supervised by the researchers Carolina Morgante and Fatima Grossi de Sá. In her work, “Genetically modified cotton plants with reduced susceptibility to root knot nematodes and drought”, Sara presented the combined strategy of overexpressing a drought tolerance gene and silencing a vital gene of the nematode Meloidogyne incognita , using RNA interference technology, to obtain plants that are less susceptible to these two stresses, simultaneously. Bruna Medeiros, who holds a master's degree in Agronomy from UnB and is a DTI-B/CNPq scholarship holder at Embrapa Cenargen, presented the work “A trans-species approach for in-root functional validation and selection of candidate genes for resistance to phytopathogens in legumes”. Supervised by the researchers Ana Brasileiro and Patrícia Guimarães, Bruna highlighted the promising results of a system for rapid functional validation of resistance genes in the roots of soybean, peanut, chickpea, common bean, cowpea, guandu, and alfalfa. More details on this research can be found in the recent publications in the journals PlosOne and Planta and in the patent filed by the team. Bruna Medeiros and Luanna Pinheiro receiving their awards from members of the organizing committee. At the same event, the students Nayara Sabrina de Freitas Alves, a doctoral student at the Federal University of Paraná, received an honorable mention for her work “Transcriptional and translational modulation via CRISPR-Cas for soybean tolerance root knot nematodes” and Luanna Pinheiro A. F. Bezerra, a doctoral student at the Catholic University of Brasília, for her work “Soybean plants tolerant to water deficit through modulation of the stress-induced programmed cell death control pathway using CRISPR/dCas9”. The two students are supervised by the researcher Fatima Grossi de Sá. The XXVII Student Talent Meeting had 150 registered participants, including undergraduate, postgraduate, and post-doctoral students. Its objectives were to publicize the work carried out at Embrapa Cenargen, in the thematic areas of Biotechnology, Biological Control, Quarantine and Genetic Resources, and to provide scientific incentives for young students and scholarship holders. Text: Carolina Morgante

< Back Coordenadora do INCT PlantStress Biotech e pesquisadora da Embrapa CENARGEN recebe prêmio no Japão A Dra. Fatima Grossi de Sá, coordenadora do INCT PlantStress Biotech e pesquisadora da Embrapa Recursos Genéticos e Biotecnologia, recebeu no dia 25 de agosto, em Kyoto, no Japão, o Certificado de Distinção pelo Conselho do Congresso Internacional de Entomologia, considerado o prêmio máximo da área no mundo. A entrega foi feita durante o XXVII International Congress of Entomology (ICE 2024). O prêmio é concedido a cada quatro anos a pesquisadores ou grupos de pesquisa com contribuição de destaque para o avanço do conhecimento em entomologia. “Sinto-me verdadeiramente honrada por ter sido nomeada”, afirmou Dra. Grossi de Sá. Seu trabalho envolve estratégias de controle de insetos praga, com ênfase nos ativos biotecnológicos e suas aplicações no desenvolvimento de produtos para o agronegócio. A Dra. Grossi de Sá é líder de pesquisa na construção de estratégias de controle de pragas, utilizando tecnologias de melhoramento genético de precisão, incluindo RNA de interferência (RNAi) e edição de genoma. Seu grupo de pesquisa vem desenvolvendo as primeiras plantas transgênicas de algodão resistentes ao bicudo do algodoeiro, atendendo a uma demanda urgente do setor produtivo e da indústria brasileira do algodão. O grupo também tem gerado plantas de soja e algodão resistentes a outras pragas agrícolas, como lagartas e nematoides formadores de galhas. Estímulo “A premiação é um grande estímulo para nós, pesquisadores, que atuamos com entusiasmo e determinação, seguindo nosso coração e nossa visão acadêmica e científica, muitas vezes nos arriscando a investigar áreas novas, emergentes e de demanda do país e dos setores diretamente ligados às nossas pesquisas. Estou orgulhosa do que venho realizando e alcançando com este trabalho”, destacou a Dra. Grossi de Sá. Ela agradeceu a todos os que a nomearam e também a todas as pessoas “incrivelmente talentosas com quem venho desenvolvendo as pesquisas ao longo dos anos.” “Essa premiação não é só minha, estende-se a todos que têm contribuído e dado suporte à minha jornada, especialmente à minha equipe e aos estudantes, cuja dedicação e paixão pela ciência têm sido cruciais para o alcance dos resultados e do sucesso”, assinalou a pesquisadora, ressaltando que esse reconhecimento será uma inspiração para jovens pesquisadores seguirem seus trabalhos com determinação e acreditando no sucesso. Texto modificado de Núcleo de Comunicação Organizacional (Embrapa Cenargen)

< Back Advances in Research from UFRJ and Embrapa on Coffee Gene that Increases Drought Resistance in Soybeans and Cotton Drought is one of the biggest challenges facing agriculture worldwide, causing significant losses, particularly in key crops such as coffee, soybeans, and cotton. Despite coffee's substantial economic importance, our understanding of how the plant responds to water scarcity at the molecular level is still limited. Research conducted by the Molecular Genetics and Plant Biotechnology Laboratory at UFRJ, led by Professor Márcio Alves-Ferreira, in collaboration with the team from Embrapa Cenargen, coordinated by researcher M. Fatima Grossi de Sa, studied all the "homeobox" (HB) gene family members present in the genome of Coffea arabica . They discovered that three of these genes are activated when the plant experiences osmotic stress (such as drought). Among these, the gene CaHB12 stood out as it is significantly activated in the leaves and roots of coffee plants under moderate and severe drought conditions. To better understand the role of CaHB12 , the scientists introduced this gene into transgenic Arabidopsis plants (a model plant) and observed that these plants exhibited increased tolerance to drought and salt during germination. During the analyses, they noted that while some classic drought response genes showed decreased activity, other pathways, such as those related to heat shock proteins, were activated, which may explain the enhanced resistance of the plants. This study was supported by the INCT PlantStress Biotech and also involved collaborations with the Universidad Nacional del Litoral (Argentina) and Universität Bonn (Germany). Furthermore, the researchers tested the expression of CaHB12 in transgenic soybean and cotton plants, with results indicating increased drought tolerance. The findings of this study, recently published in the journal Environmental and Experimental Botany , suggest that CaHB12 is a positive regulator of the stress response in coffee plants and a promising candidate for biotechnological applications. A diagram provides a graphical summary of the results. Also, check out the dissemination conducted in the FAPERJ newsletter, as well as the original article in the scientific journal. FAPERJ Dissemination https://www.faperj.br/?id=648.7.8 Link to article in EEB https://www.sciencedirect.com/science/article/abs/pii/S0098847224003411

< Back Dr. Janice de Almeida-Engler, a researcher at INRAE - France and an international collaborator of the INCT PlantStress Biotech, was elected as a corresponding member of the Brazilian Academy of Sciences Dr. Janice de Almeida-Engler, Director of Research at the Institut Sophia Agrobiotech (ISA) of INRAE-Sophie Antipolis, France, and a collaborating researcher at the INCT PlantStress Biotech , was elected as a corresponding member of the Brazilian Academy of Sciences (ABC) in the field of agricultural sciences. She was ellected on May 8, 2025, in RJ-Brazil. This honor recognizes her impactful scientific excellence and her innovative contributions to plant biotechnology, particularly through international collaborations with Brazil. Her pioneering research investigates the ontogenesis of giant cells and the mechanisms of plant infection by phytoparasitic nematodes, including induced cellular lesions. She has elucidated, in an unprecedented manner, the cell cycle and cytoskeletal dynamics involved in giant gall formation, as well as revealing communication between nuclei of giant cells via nucleotubes—features reminiscent of tumor-like cellular processes. With over 120 scientific publications and five patents, her work is crucial for advancing agricultural biotechnology. Her notable contributions include collaboration in developing plants resistant to phytonematodes through the overexpression of cell cycle inhibitors in galls and inhibiting nematode effectors by expressing double-stranded RNA (dsRNA) molecules, via RNAi technology. Her studies also identified specific gall promoters and expression modules in giant cells, currently undergoing validation for use in commodities such as tomato, soybean, and cotton, with a new intellectual property patent pending. Currently, Dr. Janice is a scientific reference in the Microscopy Platform of the PlantBIOs infrastructure at INRAE. Her work on three plant biotechnology research projects, in collaboration with Brazilian institutions, has been crucial in validating molecules aimed at increasing biomass and enhancing resistance to phytopathogens in soybean and cotton. Her leadership has fostered closer ties between ISA and EMBRAPA, Brazil’s largest agricultural research institution, promoting high-impact synergies. Furthermore, new projects are underway, and there is ongoing development of an International Associated Laboratory (LIA) with the goal of expanding and consolidating these strategic collaborations based on scientific excellence. The researcher’s nomination as a corresponding member of the ABC constitutes a formal and highly prestigious recognition of the scientist's excellence and scientific impact. This distinction highlights the relevance, innovation, and rigor of her research, while also fostering integration into international collaboration networks and advancing global scientific knowledge. Such a position elevates the researcher’s profile, strengthens academic cooperation networks, promotes knowledge exchange, and significantly contributes to scientific, technological, and innovative development. Alongside Dr. Janice de Almeida Engler, present at the nomination ceremony of ABC members were Dr. Fatima Grossi, coordinator of the INCT PlantStress Biotech ; researcher Adriana Hemerly from UFRJ; and Professor Antônio Costa de Oliveira, scientist leader of one of the INCT PlantStress Biotech’s Associated Laboratories and faculty at UFPEL-RS, who was also nominated on May 8th as a full member of the Brazilian Academy of Sciences. Nomination ceremony of the new ABC Academicians 2025, held on May 8, 2025, in RJ-Brazil, recognizing distinguished Brazilian researchers of national and international renown in the scientific community.

< Back Agricultural Technology Revolutionizes Sustainable Production Young scientists associated with the INCT PlantStress Biotech bring Embrapa's innovations to the university. In recent times, Biotechnology has accelerated the genetic advancement of plants. The techniques employed in biotechnology have enabled the development of crops through gene incorporation. These genes not only enhance productivity but also provide resistance to drought, pests, diseases, and herbicides. Until the early 2000s, over 8,000 improved varieties were created for the 11 main globally cultivated plants. The adoption of these genetically enhanced plants resulted in a 21% increase in production in all developing countries between 1961 and 1980. In the period from 1981 to 2000, this growth reached an astonishing 50%. Genetic editing and the challenge of climate change Genetic editing allows for the creation of crops resistant to pests and diseases, reducing dependence on environmentally harmful pesticides. Additionally, it is feasible to develop plants that thrive in adverse conditions, such as acidic soils or water scarcity, making agriculture more adaptable to climate changes. In this way, for each plant species, there is a wide variety of cultivars, each with characteristics that favor their productivity in specific regions. This is why we can cultivate grapes, for example, in different regions of Brazil, such as the South, Southeast, and Northeast. Genetic editing is already generating new varieties better adapted to current climate conditions and will continue to enhance agriculture's productivity and climate resilience in the coming decades. The benefits of this approach go beyond adapting to climate change. New cultivars developed with CRISPR technology, for example, also play a fundamental role in reducing greenhouse gas emissions, decreasing the use of agricultural inputs, and preventing food waste, factors that support environmentally friendly agriculture. Sharing ideas and disseminating knowledge The integration of advanced technologies into agricultural sciences has become a topic of increasing relevance, offering significant opportunities to improve the production of natural resources, such as food, fibers, and renewable energy, while striving to protect and preserve these crucial resources for present and future generations. To promote the discussion of these topics and disseminate knowledge among students and future agribusiness professionals, the XVIII Agricultural Sciences Week, IX Research and Graduate Studies Conference in Plant Production, and VII Plant Genetics and Cytogenetics Conference had the theme "The Challenges of Incorporating Technological Tools: Production and Preservation of Natural Resources." The event, held at the South Campus of the State University of Goiás (UEG) from October 2nd to 6th, 2023, brought together professors, students, and technicians from the Ipameri Unit and other UEG units, as well as representatives from local and regional commercial institutions. During the event, Dr. Thuanne Pires Ribeiro, MSc. Luanna Pinheiro de Albuquerque Freitas Bezerra, and MSc. Valdeir Junio Vaz Moreira conducted the course "Genome Editing for Bioproduct Production," in which they taught university students the most advanced techniques for genetic transformation and editing in cultivated plants. Covering everything from fundamental principles of biology to the development of protocols for obtaining transgenic plants, these young researchers shared their knowledge and inspired students in the fields of Agronomy and Forestry Engineering at the institution.

< Back Underwater 'breathing' plants could be key to stress-resistant crops Wetland plants have a high tolerance against flooding due to the formation of "lysigenous aerenchyma," air channels that help transfer gases to the submerged roots. These channels also help the plant withstand drought and nutrient deficiency. Now, scientists from Japan investigate the underlying mechanism of aerenchyma formation to understand the phenomenon better, opening doors to the development of crops that are resilient against extreme weather changes. Floods and droughts are the main environmental disasters responsible for most crop failures. Aerenchyma formation can help crops cope with these environmental stresses. However, it is not commonly observed in non-wetland species like wheat and maize, which are staple food crops in certain areas of the world. Researchers Takaki Yamauchi and Mikio Nakazono from Nagoya University, Japan, have surveyed literature on the topic to get a concrete overview of the various factors involved in aerenchyma formation. "If we can genetically control the timing and amount of lysigenous aerenchyma formation in roots of all agronomically important crops, such as maize, wheat and soybean, the global crop production loss could be dramatically reduced," says Dr. Nakazono. Dr. Yamauchi and Dr. Nakazono suggest imagining the lysigenous aerenchyma to a snorkel used to breathe underwater. During flooding, the roots get cut off from oxygen and other vital gases needed for survival. In response, the plant creates air pathways connecting the submerged regions of the plant to the parts above water. Similar to a snorkel, these pathways help the plant "breathe" by transporting gases to the submerged roots. Moreover, the air channels reduce the energy requirement for the breathing process and can help the plant conserve energy during extreme conditions of drought or nutrient deficit. The researchers found that a phytohormone called "auxin" is required for the formation of aerenchyma during normal root growth, and identified two factors leading to the induction of aerenchyma formation in response to flooding. The phenomenon begins when the roots are submerged underwater in aerobic conditions. The restrictions to gas exchange cause ethylene to accumulate in the roots, which encourage the production of respiratory burst oxidase homolog (RBOH) -- an enzyme responsible for reactive oxygen species (ROS) production. As it turns out, the released ROS triggers cell death in the tissues, forming cavities for the passage of gases. The RBOH can also be activated by the presence of calcium (Ca2+) ions that are transported from the apoplast (water pathways). Certain plants have calcium-dependent protein kinases that use Ca2+ to add phosphates to the RBOH, stimulating it to produce ROS. This effect occurs at later stages as the plants gradually experience oxygen-deficient conditions after prolonged underwater submersion. While aerenchyma is mostly associated with plants that have adapted to soils with high water content, it can also develop in upland plants under drought and nutrient deficiency. Low concentrations of nitrogen and phosphorus, essential nutrients required for plant growth, was found to increase the ethylene sensitivity, stimulating the formation of aerenchyma. Moreover, ethylene was also a common factor in triggering aerenchyma in maize, offering a way to improve the crop's resilience. "The increase in ethylene sensitivity could be an effective strategy to stimulate aerenchyma formation in the absence of restricted gas diffusion," speculates Dr. Yamauchi. While the mechanism behind aerenchyma formation remains uncertain, suggesting the need for further research, the findings of this study open up the possibility of improving crop resilience and paving the way for better food security in the wake of climate change. The new paper has been based on the following two papers: "Fine control of aerenchyma and lateral root development through AUX/IAA- and ARF-dependent auxin signaling." Proceedings of the National Academy of Sciences of the United States of America , 116, 2019, DOI: 10.1073/pnas.1907181116 "An NADPH oxidase RBOH functions in rice roots during lysigenous aerenchyma formation under oxygen-deficient conditions." The Plant Cell , 29, 2017, DOI: 10.1105/tpc.16.00976 Funding Information: This study was supported by the Japan Science and Technology Agency PRESTO grants JPMJPR17Q8 to T.Y. and Grant-in-Aid for Transformative Research Areas (A) (MEXT KAKENHI grant JP20H05912) to M.N. Source: Materials provided by Nagoya University . Note: Content may be edited for style and length.

< Back INCT PlantStress results are presented in Symposium Between the 23th and 27th of July 2023, the Siconbiol - 17th Symposium on Biological Control and 2nd Latin American Symposium on Biological Control promoted by the Entomological Society of Brazil (SEB) took place in the cities of Juazeiro-BA and Petrolina - PE. Considered the largest event on biological control in Latin America, it brought together researchers, students, and professionals from the productive sector. The event addressed the latest advances in different areas of biological control, such as the use of bacteria, fungi, viruses, nematodes, parasitoids, predators and much more in the control of pests and diseases. Although the agricultural area is still the largest audience, the event also covers the health area, mainly with the increased use of biological control of medically important insects. Presentation of INCT PlantStress Biotech results During the event, one of the lectures was given by Dr. Carolina Morgante, who presented the results obtained by the research group of the Plant-Pest Interaction Laboratory (LIMPP) of Embrapa/Cenargen and which is linked to the INCT PlantStress. Dr Carolina showed important results that can be used in Integrated Pest Management (IPM) as cutting-edge tools for insect control. The use of genetic engineering, with genetic transformation techniques and RNAi technology are among the strategies used by researchers. Together with the productive sector, INCT PlantStress is always developing technologies for more profitable and sustainable agricultural production.

The INCT PlantStress Biotech develops technologies to overcome climatic barriers. The INCT network is formed by national and international research groups, developing biotechnological assets applied to hidric stress and pest control relevant crops for agribusiness. PlantStress Biotech INCT Biotechnological Assets Applied to Drought and Pests in Relevant Crops for Agribusiness About Us Main biotic and abiotic stresses we work Boll weevil Anthomonus grandis Nematode Meloidogyne incognita Meloidogyne javanica Drought Drought tolerance genes Caterpillars Helicoverpa armigera Spodoptera frugiperda What We Do The National Institute of Science and Technology, PlantStress Biotech INCT, integrates various Brazilian research groups and international partners, experts in plant physiology, transcriptomic, epigenetic, proteomic, bioinformatic and functional genomics analyses. The integrative research group represents a multidisciplinary and multi-institutional network with national and international excellence to generate innovative biotechnologies applied to corn, soybean and cotton focused on the tolerance to deficit hydric and pest control (Meloidogyne spp, H. armigera and S. frugiperda ). The project includes bioprospection, isolation, characterization, and functional validation of genes/molecules involved in plant pest resistance and drought tolerance. Read More Our Goals Focusing on agribusiness, the INCT PlantStress Biotech has significantly contributed to boosting productivity, sustainability, and competitiveness in the sector. Its research spans from improvements in agricultural practices to the development of innovative technologies. Beyond drought stress and pest control, the generated biotechnological assets hold the potential to influence other critical traits like seed and fruit quality, nutritional value enhancement, and more. This holistic approach positions the INCT as a cornerstone for the advancement of the Brazilian agribusiness, promising a future where innovation and sustainability coalesce for the benefit of society at large. Read More A high-impact program for Brazilian Agriculture 400+ Published Scientific Papers 30+ Researchers Involved 24+ Dev eloped Paten ts 10 Brazilian Research Units 25 Abroad Instituition 60+ Master's 80+ Ph.Ds trained NEWS Highlighting the INCT PlantStress Biotech’s Impact at the 2025 International Symposium on Rice Functional Genomics (ISRFG 2025) Read Ver mais Highlight of the INCT PlantStress Biotech at the International Congress of the Brazilian Genetics Society – Genetica 2025 Read Ver mais World's Best Scientists 2025- Research.com Read Ver mais Read All SCIENTIFIC EVENTS 8th Brazilian Biotechnology Congress 2025 - Biotech for Sustainable Agriculture 19 - 22 October 2025 More information See All WHERE ARE WE? Associate laboratories Partnerships PlantStress Biotech INCT Contact

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Discover how we are turning curiosity into innovation, fostering excellence in scientific research. News Highlighting the INCT PlantStress Biotech’s Impact at the 2025 International Symposium on Rice Functional Genomics (ISRFG 2025) Participation of the INCT PlantsStress Biotech at the 2025 International Symposium on Rice Functional Genomics (ISRFG 2025), held from August 23-27, 2025, in Chengdu, China, under the theme “ Green and Nutritional Rice for Human Health and Sustainability” . Read More Highlight of the INCT PlantStress Biotech at the International Congress of the Brazilian Genetics Society – Genetica 2025 At the International Congress of the Brazilian Genetics Society – Genetica 2025, held in Belém, Pará, from August 13 to 16, 2025, the INCT PlantStress Biotech team led a dynamic session titled “Strategies for Mitigating Abiotic and Biotic Stress in Agriculture.” Read More World's Best Scientists 2025- Research.com Twenty-four researchers from Embrapa are among the most cited in the world, according to an international Research.com ranking. Read More Dr. Janice de Almeida-Engler, a researcher at INRAE - France and an international collaborator of the INCT PlantStress Biotech, was elected as a corresponding member of the Brazilian Academy of Sciences Dr. Janice de Almeida-Engler, Director of Research at the Institut Sophia Agrobiotech (ISA) of INRAE-Sophie Antipolis, France, and a collaborating researcher at the INCT PlantStress Biotech, was elected as a corresponding member of the Brazilian Academy of Sciences (ABC) in the field of agricultural sciences. She was ellected on May 8, 2025, in RJ-Brazil. This honor recognizes her impactful scientific excellence and her innovative contributions to plant biotechnology, particularly through international collaborations with Brazil. Read More Innovative research developed by INCT PlantStress Biotech was showcased during the IX Brazilian Symposium on Molecular Plant Genetics (IX SBGMP). Valdeir Moreira, a postdoctoral fellow under the supervision of Dr. Fatima Grossi, presented his research entitled “GmGLP10-overexpressing transgenic plants trigger defense responses in Meloidogyne incognita-induced giant cells by upregulation of H2O2-sensitive genes.” during the IX Brazilian Symposium on Molecular Plant Genetics (IX SBGMP), held in Búzios, Rio de Janeiro, from May 27 to 30. Read More 13/02/2025 8th Brazilian Biotechnology Congress We are honored to invite you to participate in the 8th Brazilian Biotechnology Congress, which will be held from October 19 to 22, 2025, in Natal, RN, Brazil. Read More 28/01/2024 INCT PlantStress Biotech research shines at PAG32. INCT PlantStress Biotech research shines at the Plant and Animal Genome Conference (PAG32) in San Diego, January 10-15th, USA. Read More 28/01/2024 O Tomate Roxo GM - rico em antocioninas Palestra destaque no evento PAG 32 de Cathie Martin, responsável pela produção do Tomate Roxo Geneticamente Modificado, que aumenta os benefícios à saúde e enriquece os efeitos nutritivos. Read More 15/10/2024 INCT PlantStress Biotech: Disseminating Knowledge in Educational Institutions Dr. Ana Cristina Miranda Brasileiro from Embrapa – Cenargen has shared knowledge and technological innovations from INCT PlantStress Biotech with society. She participates in science education programs for elementary and high school students in the Distrito Federal (DF). Read More 15/10/2024 Advances in Research from UFRJ and Embrapa on Coffee Gene that Increases Drought Resistance in Soybeans and Cotton The research was conducted by the Molecular Genetics and Plant Biotechnology Laboratory at UFRJ, led by Professor Márcio Alves-Ferreira, in collaboration with the team from Embrapa Cenargen, coordinated by researcher M. Fatima Grossi de Sa. Read More 15/10/2024 INCT PlantStress Biotech’s Participation in the International Congress of Nematology (ICN) in Antibes Juan-Les-Pins, France Professor Robert Miller (UnB), a member of the INCT PlantStress Biotech, participated in the 7th International Congress of Nematology (ICN), between the 1st and 6th of May 2022. Read More 11/10/2024 INCT PlantStress Biotech’s Participation in the Crop Genomics for Global Food Security Workshop at PAG 2024 Professor Robert Miller (UnB), a member of the INCT PlantStress Biotech, participated in the 31st Plant and Animal Genome Conference (PAG) in San Diego, California, USA between 12-17 January 2024. Read More 25/09/2024 Coordinator of the INCT PlantStress Biotech participated in the RNAi Discussion Forum during the Brazilian Congress of Entomology Participation in the Forum 'RNAi: uma ferramenta poderosa no controle sustentável de pragas agrícolas' during the XXIX Brazilian Congress of Entomology (CBE) 2024, held in Uberlândia, MG, Brazil, from September 22 to 26, 2024. Read More 24/09/2024 Antonio Costa de Oliveira, researcher at INCT PlantStress Biotech, participated in international scientific events Participation of Antonio Costa de Oliveira in the scientific events: BIOIBEROAMERICA in Monterrey, Mexico, from September 3 to 6, 2024, and ISRFG2024 (International Symposium on Rice Functional Genomics, 2024) in Little Rock, Arkansas, USA, from September 9 to 11, 2024. Read More 07/09/2024 INCT PlantStress Biotech research highlighted at a student meeting The research carried out at the INCT PlantStress Biotech was highlighted during the XXVII Student Talent Meeting, held at Embrapa Recursos Genéticos e Biotecnologia (Cenargen), in Brasília. Read More 07/09/2024 Pesquisa do INCT PlantStress Biotech é destaque em encontro estudantil A pesquisa realizada no INCT PlantStress Biotech foi destaque durante o XXVII Encontro do Talento Estudantil, ocorrido na Embrapa Recursos Genéticos e Biotecnologia (Cenargen), em Brasília. Read More 06/09/2024 INCT PlantStress Biotech Coordinator Receives World’s Top Entomology Award Dr. Fatima Grossi de Sá received the Certificate of Distinction from the International Congress of Entomology Council in Kyoto, Japan, considered the highest honor in the field worldwide. Read More 06/09/2024 Coordenadora do INCT PlantStress Biotech e pesquisadora da Embrapa CENARGEN recebe prêmio no Japão Dra. Fatima Grossi de Sá recebeu em Kyoto, Japão, o Certificado de Distinção pelo Conselho do Congresso Internacional de Entomologia, considerado o prêmio máximo da área no mundo. Read More 14/08/2024 CGF5: nova linhagem de trigo para o cerrado, resistente ao brusone Nova linhagem de trigo resistente ao brusone é desenvolvida por pesquisadores do INCT PlantStress Biotech Read More 21/11/2023 The 2023 Annual LIMPP Workshop: Focus on Student Engagement The LIMPP Workshop takes place annually and aims to train students and discuss results with them. Read More 11/10/2023 Agricultural Technology Revolutionizes Sustainable Production Young scientists associated with the INCT PlantStress Biotech bring Embrapa's innovations to the university. Read More 21/08/2023 Research Developing Nematode Control Alternatives Receives Awards at Conference Students Affiliated with INCT Plant Stress Biotech, Developing Nematode Control Alternatives, Win Best Research Awards at the 53rd Brazilian Phytopathology Congress. Read More 02/08/2023 INCT PlantStress results are presented in Symposium The Embrapa researcher Dr. Carolina Morgante presented some results of biotechonology use in pests control in Siconbiol - 17th Symposium on Biological Control and 2nd Latin American Symposium on Biological Control. Read More 25/07/2023 Biotechnology generates alternatives to climate change Using genetic engineering, a biotechnologist develops drought-tolerant soybean plants and receives awards. Read More 12/07/2023 INCT PlantStress Biotech Workshop 2023 On July 10th and 11th, the IV Workshop of the PlantStress Biotech INCT took place at Embrapa Genetic Resources and Biotechnology in Brasília, DF. This annual workshop is held for the presentation and discussion of research results conducted by INCT PlantStress. In 2023, the workshop was attended by the leaders of the associated laboratories, fellows, and researchers from the work development team. Read More 10/08/2022 Dr. Marcio Alves-Ferreira talked about his latest paper in Plant Biology 2022 Conference During the first talk, Chair Dr. Marcio Alves-Ferreira, from the universidade federal do Rio de Janeiro, Brazil, talked about his latest paper in Current Plant Biology. They aimed to identify molecular players mediating the defense response of cotton (Gossypium spp) to the Cotton Boll Weevil (CBW, Anthonomus grandis)... Read More 17/09/2021 CAPES Thesis Award 2021 Thesis awarded by Capes validates an improvement system by genome editing and presents a new protocol for soybean transformation... Read More 27/05/2022 Science uses genetics against plant parasites Valdeir Junio Vaz Moreira pode ter encontrado um novo caminho para combater pragas parasitas, chamadas nematoides, em plantas como algodão e soja. Bioquímico e aluno do Programa de Biologia Molecular da Universidade de Brasília (UnB), ele desenvolve sua pesquisa em um doutorado-sanduíche no Institut National de la Recherche Agronomique (INRAe), na França, com bolsa do Programa CAPES-Cofecub... Read More 10/02/2022 More efficient maize growth Maize has a significantly higher productivity rate compared with many other crops. The particular leaf anatomy and special form of photosynthesis (referred to as 'C4') developed during its evolution allow maize to grow considerably faster than comparable plants... Read More 10/02/2022 Underwater 'breathing' plants could be key to stress-resistant crops Wetland plants have a high tolerance against flooding due to the formation of "lysigenous aerenchyma," air channels that help transfer gases to the submerged roots. These channels also help the plant withstand drought and nutrient deficiency... Read More 10/02/2022 Genetic engineering can have a positive effect on the climate Agriculture accounts for around 25 percent of all greenhouse gas emissions worldwide. A large share of these emissions is due to livestock production and fertilizer use. However, more than one-third of agriculture's emissions is caused by land-use change, especially the conversion of forests and other nature reserves to agricultural land in order to satisfy the rising global demand for food and feed... Read More Highlighting the INCT PlantStress Biotech’s Impact at the 2025 International Symposium on Rice Functional Genomics (ISRFG 2025) Participation of the INCT PlantsStress Biotech at the 2025 International Symposium on Rice Functional Genomics (ISRFG 2025), held from August 23-27, 2025, in Chengdu, China, under the theme “ Green and Nutritional Rice for Human Health and Sustainability” . Read More Highlight of the INCT PlantStress Biotech at the International Congress of the Brazilian Genetics Society – Genetica 2025 At the International Congress of the Brazilian Genetics Society – Genetica 2025, held in Belém, Pará, from August 13 to 16, 2025, the INCT PlantStress Biotech team led a dynamic session titled “Strategies for Mitigating Abiotic and Biotic Stress in Agriculture.” Read More World's Best Scientists 2025- Research.com Twenty-four researchers from Embrapa are among the most cited in the world, according to an international Research.com ranking. Read More Dr. Janice de Almeida-Engler, a researcher at INRAE - France and an international collaborator of the INCT PlantStress Biotech, was elected as a corresponding member of the Brazilian Academy of Sciences Dr. Janice de Almeida-Engler, Director of Research at the Institut Sophia Agrobiotech (ISA) of INRAE-Sophie Antipolis, France, and a collaborating researcher at the INCT PlantStress Biotech, was elected as a corresponding member of the Brazilian Academy of Sciences (ABC) in the field of agricultural sciences. She was ellected on May 8, 2025, in RJ-Brazil. This honor recognizes her impactful scientific excellence and her innovative contributions to plant biotechnology, particularly through international collaborations with Brazil. Read More Innovative research developed by INCT PlantStress Biotech was showcased during the IX Brazilian Symposium on Molecular Plant Genetics (IX SBGMP). Valdeir Moreira, a postdoctoral fellow under the supervision of Dr. Fatima Grossi, presented his research entitled “GmGLP10-overexpressing transgenic plants trigger defense responses in Meloidogyne incognita-induced giant cells by upregulation of H2O2-sensitive genes.” during the IX Brazilian Symposium on Molecular Plant Genetics (IX SBGMP), held in Búzios, Rio de Janeiro, from May 27 to 30. Read More 13/02/2025 8th Brazilian Biotechnology Congress We are honored to invite you to participate in the 8th Brazilian Biotechnology Congress, which will be held from October 19 to 22, 2025, in Natal, RN, Brazil. Read More 28/01/2024 INCT PlantStress Biotech research shines at PAG32. INCT PlantStress Biotech research shines at the Plant and Animal Genome Conference (PAG32) in San Diego, January 10-15th, USA. Read More 28/01/2024 O Tomate Roxo GM - rico em antocioninas Palestra destaque no evento PAG 32 de Cathie Martin, responsável pela produção do Tomate Roxo Geneticamente Modificado, que aumenta os benefícios à saúde e enriquece os efeitos nutritivos. Read More 15/10/2024 INCT PlantStress Biotech: Disseminating Knowledge in Educational Institutions Dr. Ana Cristina Miranda Brasileiro from Embrapa – Cenargen has shared knowledge and technological innovations from INCT PlantStress Biotech with society. She participates in science education programs for elementary and high school students in the Distrito Federal (DF). Read More 15/10/2024 Advances in Research from UFRJ and Embrapa on Coffee Gene that Increases Drought Resistance in Soybeans and Cotton The research was conducted by the Molecular Genetics and Plant Biotechnology Laboratory at UFRJ, led by Professor Márcio Alves-Ferreira, in collaboration with the team from Embrapa Cenargen, coordinated by researcher M. Fatima Grossi de Sa. Read More 15/10/2024 INCT PlantStress Biotech’s Participation in the International Congress of Nematology (ICN) in Antibes Juan-Les-Pins, France Professor Robert Miller (UnB), a member of the INCT PlantStress Biotech, participated in the 7th International Congress of Nematology (ICN), between the 1st and 6th of May 2022. Read More 11/10/2024 INCT PlantStress Biotech’s Participation in the Crop Genomics for Global Food Security Workshop at PAG 2024 Professor Robert Miller (UnB), a member of the INCT PlantStress Biotech, participated in the 31st Plant and Animal Genome Conference (PAG) in San Diego, California, USA between 12-17 January 2024. Read More 25/09/2024 Coordinator of the INCT PlantStress Biotech participated in the RNAi Discussion Forum during the Brazilian Congress of Entomology Participation in the Forum 'RNAi: uma ferramenta poderosa no controle sustentável de pragas agrícolas' during the XXIX Brazilian Congress of Entomology (CBE) 2024, held in Uberlândia, MG, Brazil, from September 22 to 26, 2024. Read More 24/09/2024 Antonio Costa de Oliveira, researcher at INCT PlantStress Biotech, participated in international scientific events Participation of Antonio Costa de Oliveira in the scientific events: BIOIBEROAMERICA in Monterrey, Mexico, from September 3 to 6, 2024, and ISRFG2024 (International Symposium on Rice Functional Genomics, 2024) in Little Rock, Arkansas, USA, from September 9 to 11, 2024. Read More 07/09/2024 INCT PlantStress Biotech research highlighted at a student meeting The research carried out at the INCT PlantStress Biotech was highlighted during the XXVII Student Talent Meeting, held at Embrapa Recursos Genéticos e Biotecnologia (Cenargen), in Brasília. Read More 07/09/2024 Pesquisa do INCT PlantStress Biotech é destaque em encontro estudantil A pesquisa realizada no INCT PlantStress Biotech foi destaque durante o XXVII Encontro do Talento Estudantil, ocorrido na Embrapa Recursos Genéticos e Biotecnologia (Cenargen), em Brasília. Read More 06/09/2024 INCT PlantStress Biotech Coordinator Receives World’s Top Entomology Award Dr. Fatima Grossi de Sá received the Certificate of Distinction from the International Congress of Entomology Council in Kyoto, Japan, considered the highest honor in the field worldwide. Read More 06/09/2024 Coordenadora do INCT PlantStress Biotech e pesquisadora da Embrapa CENARGEN recebe prêmio no Japão Dra. Fatima Grossi de Sá recebeu em Kyoto, Japão, o Certificado de Distinção pelo Conselho do Congresso Internacional de Entomologia, considerado o prêmio máximo da área no mundo. Read More 14/08/2024 CGF5: nova linhagem de trigo para o cerrado, resistente ao brusone Nova linhagem de trigo resistente ao brusone é desenvolvida por pesquisadores do INCT PlantStress Biotech Read More 21/11/2023 The 2023 Annual LIMPP Workshop: Focus on Student Engagement The LIMPP Workshop takes place annually and aims to train students and discuss results with them. Read More 11/10/2023 Agricultural Technology Revolutionizes Sustainable Production Young scientists associated with the INCT PlantStress Biotech bring Embrapa's innovations to the university. Read More 21/08/2023 Research Developing Nematode Control Alternatives Receives Awards at Conference Students Affiliated with INCT Plant Stress Biotech, Developing Nematode Control Alternatives, Win Best Research Awards at the 53rd Brazilian Phytopathology Congress. Read More 02/08/2023 INCT PlantStress results are presented in Symposium The Embrapa researcher Dr. Carolina Morgante presented some results of biotechonology use in pests control in Siconbiol - 17th Symposium on Biological Control and 2nd Latin American Symposium on Biological Control. Read More 25/07/2023 Biotechnology generates alternatives to climate change Using genetic engineering, a biotechnologist develops drought-tolerant soybean plants and receives awards. Read More 12/07/2023 INCT PlantStress Biotech Workshop 2023 On July 10th and 11th, the IV Workshop of the PlantStress Biotech INCT took place at Embrapa Genetic Resources and Biotechnology in Brasília, DF. This annual workshop is held for the presentation and discussion of research results conducted by INCT PlantStress. In 2023, the workshop was attended by the leaders of the associated laboratories, fellows, and researchers from the work development team. Read More 10/08/2022 Dr. Marcio Alves-Ferreira talked about his latest paper in Plant Biology 2022 Conference During the first talk, Chair Dr. Marcio Alves-Ferreira, from the universidade federal do Rio de Janeiro, Brazil, talked about his latest paper in Current Plant Biology. They aimed to identify molecular players mediating the defense response of cotton (Gossypium spp) to the Cotton Boll Weevil (CBW, Anthonomus grandis)... Read More 17/09/2021 CAPES Thesis Award 2021 Thesis awarded by Capes validates an improvement system by genome editing and presents a new protocol for soybean transformation... Read More 27/05/2022 Science uses genetics against plant parasites Valdeir Junio Vaz Moreira pode ter encontrado um novo caminho para combater pragas parasitas, chamadas nematoides, em plantas como algodão e soja. Bioquímico e aluno do Programa de Biologia Molecular da Universidade de Brasília (UnB), ele desenvolve sua pesquisa em um doutorado-sanduíche no Institut National de la Recherche Agronomique (INRAe), na França, com bolsa do Programa CAPES-Cofecub... Read More 10/02/2022 More efficient maize growth Maize has a significantly higher productivity rate compared with many other crops. The particular leaf anatomy and special form of photosynthesis (referred to as 'C4') developed during its evolution allow maize to grow considerably faster than comparable plants... Read More 10/02/2022 Underwater 'breathing' plants could be key to stress-resistant crops Wetland plants have a high tolerance against flooding due to the formation of "lysigenous aerenchyma," air channels that help transfer gases to the submerged roots. These channels also help the plant withstand drought and nutrient deficiency... Read More 10/02/2022 Genetic engineering can have a positive effect on the climate Agriculture accounts for around 25 percent of all greenhouse gas emissions worldwide. A large share of these emissions is due to livestock production and fertilizer use. However, more than one-third of agriculture's emissions is caused by land-use change, especially the conversion of forests and other nature reserves to agricultural land in order to satisfy the rising global demand for food and feed... Read More

Learn more about the project coordinator, her resume and recent publications. Maria Fatima Grossi-de-Sa Coordinator PlantStress Biotech INCT Researcher Group Leader at Embrapa Genetic Resources and Biotechnology Lattes: http://lattes.cnpq.br/3058512809761818 E-mail: fatima.grossi@embrapa.br Phone number: +55 (61) 3448-4902 PROFILE Maria Fatima Grossi-de-Sa is a Researcher Group Leader at EMBRAPA Genetic Resources and Biotechnology and Professor at the Catholic University of Brasília, Brasília-DF, Brazil. She is also the Coordinator of the National Institute of Science and Technology – PlantStress Biotech INCT at EMBRAPA and Director President of the Brazilian Society of Biotechnology. She is a full member of the Brazilian Academy of Sciences and the World Academy of Science (TWAS). In 2018, she received the Commander medal of the National Order of Scientific Merit. She works in a wide range of research interests with an emphasis on plant biotechnology. Her main research interests are: plant-pest molecular interactions, focused on phytonematodes and insect-pests; development of GM crop plants for tolerance/resistance to abiotic and biotic stresses; understanding the molecular RNAi mechanism on insect pests, and; development of biopharmaceuticals. She is classified as scientific researcher 1A at the Brazilian National Research Council (CNPq), and has coordinated several research projects finnaced by EMBRAPA, FAP-DF, CAPES, and CNPq. Currently, her research group is funded by industries and the productive sector of agribusiness. EDUCATION B.Sc. In Biological Sciences, University of Brasilia, Brazil, 1979. M.Sc. In Molecular Biology, University of Brasilia, Brazil, 1982. Ph.D. University PARIS VII, Paris, France, 1987. CURRENT POSITION Researcher Leader on Plant Biotechnology at EMBRAPA Genetic Resources and Biotechnology - Brasilia- DF, Brazil (since 1989). Senior Associate Professor at Catholic University of Brasilia (UCB), Brasilia-DF, Brazil (since 2004). President of Brazilian Society of Biotechnology (since 2016). Director of the National Institute Science & Technology on Plant Stress Biotechnology (INCT PlantStress Biotech; since 2016). HONOURS/AWARDS Award of Excellence in recognition of Outstanding Research Performance by Embrapa (2009). Award Brazil SCOPUS Awards 2010 - Elsevier/CAPES (2010). Full elected member of Brazilian Academy of Sciences (Elected May 2011). Full elected member of the World Academy of Sciences (Elected Nov 2014). National Order of Scientific Merit - Commander Class, Ministry of Science and Technology - Presidency of the Republic (2018). RESEARCH INTERESTS The main research interest focuses on novel and innovative approaches to crop protection, plant-nematode and insect interactions, transgenic crops, biotic and abiotic stresses, plant genome editing, gene silencing, insect nanobiotechnology, biopesticides based on RNA interference approaches, and recombinant proteins. RELEVANT PAPERS (LAST 7 YEARS) 2023 ATELLA, A. L.; Grossi-de-Sa, M. F.; Alves-Ferreira, M. (2023). Cotton promoters for controlled gene expression. Electronic Journal of Biotechnology, v. 62, p. 10.1016/j.ejbt. https://doi.org/10.1016/j.ejbt.2022.12.002 BASSO, M. F.; Lourenço-Tessutti, I. T.; Moreira-Pinto, C. E.; Mendes, R. A. G.; Pereira, D. G.; Grandis, A.; Macedo, L. L. P.; Macedo, A. F.; Gomes, A. C. M. M.; Arraes, F. B. M.; Togawa, R. C.; do Carmo Costa, M. M.; Marcelino-Guimaraes, F. C.; Silva, M. C. M.; Floh, E. I. S.; Buckeridge, M. S., de Almeida Engler, J., Grossi-de-Sa, M. F. (2023). Overexpression of the GmEXPA1 gene reduces plant susceptibility to Meloidogyne incognita . Plant Cell Report , v. 42, n. 1, p. 137-152. https://doi.org/10.1007/s00299-022-02941-3 TRENZ, T. S.; Turchetto-Zolet, A. C.; Margis, R.; Margis-Pinheiro, M.; Maraschin, F. S. (2023) . Functional analysis of alternative castor bean DGAT enzymes. Genetics and Molecular Biology (Online Version), v. 46, p. 1-12. https://doi.org/10.1590/1678-4685-GMB-2022-0097 2022 ARRAES, F.B.M.; Vasquez, D.D.N.; Tahir, M.; Pinheiro, D.H.; Faheem, M.; Freitas-Alves, N.S.; Moreira-Pinto, C.E.; Moreira, V.J.V.; Paes-de-Melo, B.; Lisei-de-Sa, M.E.; Morgante, C.V.; Mota, A.P.Z.; Lourenço-Tessutti, I.T.; Togawa, R.C.; Grynberg, P.; Fragoso, R.R.; de Almeida-Engler, J.; Larsen, M.R.; Grossi-de-Sa, M.F. (2022). Integrated omic approaches reveal molecular mechanisms of tolerance during soybean and Meloidogyne incognita interactions. Plants , v. 11, n. 2744. https://doi.org/10.3390/plants11202744 BASSO, M.F.; Lourenço-Tessutti, I.T.; Moreira-Pinto, C.E.; Mendes, R.A.G.; Pereira, D.G.; Grandis, A. Macedo, L.L.P.; Macedo, A.F.; Gomes, A.C.M.M.; Arraes, F.B.M.; Togawa, R.C.; Costa, M.M.C.C.; Marcelino-Guimaraes, F.C.; Silva, M.C.M.; Floh, E.I.S.; Buckeridge, M.S.; de Almeida Engler, J.; Grossi-de-Sa, M.F. (2022). Overexpression of the GmEXPA1 gene reduces plant susceptibility to Meloidogyne incognita . Plant Cell Reports . https://doi.org/10.1007/s00299-022-02941-3 FRAGOSO, R.R.; Arraes, F.B.M.; Lourenço-Tessutti, I.T.; Miranda, V.J.; Basso, M.F.; Ferreira, A.V.J.; Viana, A.A.B.; Lins, C.B.J.; Lins, P.C.; Moura, S.M.; Batista, J.A.N.; Silva, M.C.M.; Engler, G.; Morgante, C.V.; Lisei-de-Sa, M.E.; Vasques, R.M.; de Almeida-Engler J.; Grossi-de-Sa, M.F. (2022). Functional characterization of the pUceS8.3 promoter and its potential use for ectopic gene overexpression. Planta , v. 256, n. 4, p. 1-18. https://doi.org/10.1007/s00425-022-03980-6 HABIBI, P.; Shi, Y.; Fatima Grossi-de-Sa, M. ; Khan I. (2022). Plants as sources of natural and recombinant antimalaria agents. Molecular Biotechnology . https://doi.org/10.1007/s12033-022-00499-9 KARALIJA, E.; Vergata, C.; Basso, M.F.; Negussu, M.; Zaccai, M.; Grossi-de-Sa, M.F. ; Martinelli, F. Chickpeas’. (2022). Tolerance of Drought and Heat: Current Knowledge and Next Steps. Agronomy , v. 12, n. 2248. https://doi.org/10.3390/agronomy12102248 MENDES, R.A.G.; Basso, M.F.; Amora, D.X.; Silva, A.P.; Paes-de-Melo, B.; Togawa, R.C.; Albuquerque, E.V.S.; Lisei-de-Sa, M.E.; Macedo, L.L.P.; Lourenço-Tessutti, I.T.; Grossi-de-Sa, M.F. (2022). In planta RNAi approach targeting three M. incognita effector genes disturbed the process of infection and reduced plant susceptibility. Experimental Parasitolog y, v. 238, p. 108246, https://doi.org/10.1016/j.exppara.2022.108246 MOREIRA, V.J.V.; Lourenço-Tessutti, I.T.; Basso, M.F.; Lisei-de-Sa, M.E.; Morgante, C.V.; Paes-de-Melo, B.; Arraes, F.B.M.; Martins-de-Sa, D.; Silva, M.C.M.; de Almeida Engler, J.; Grossi-de-Sa, M.F. (2022). Minc03328 effector gene downregulation severely affects Meloidogyne incognita parasitism in transgenic Arabidopsis thaliana . Planta , v. 255, p. 44-59. https://doi.org/10.1007/s00425-022-03823-4 MOURA, S.M.; Babilonia, K.; de Macedo, L.L.P.; Grossi-de-Sá, M.F. ; Shan, L.; He, P.; Alves-Ferreira, M. (2022). The oral secretion from Cotton Boll Weevil (Anthonomus grandis) induces defense responses in cotton (Gossypium spp) and Arabidopsis thaliana . Current Plant Biology , p. 100250. https://doi.org/10.1016/j.cpb.2022.100250 MOURA, S.M.; Freitas, E.O.; Ribeiro, T.P.; Paes-de-Melo, B.; Arraes, B.M.F.; Macedo, L.L.P.; Paixão, J.F.R.; Lourenço-Tessutti, I.T.; Artico, S.; Valença, D.C.; Silva, M.C.M.; Oliveira, A.C.; Alves-Ferreira, M.; Grossi-de-Sa, M.F. (2022). Discovery and functional characterization of novel cotton promoters with potential application to pest control. Plant Cell Reports , v. 41, p. 1589–1601. https://doi.org/10.1007/s00299-022-02880-z REIS, M.A.; Noriega, D.D.; dos Santos Alves, G.; Coelho, R.R.; Grossi-de-Sa, M.F. ; Antonino, J.D. (2022). Why is oral-induced RNAi inefficient in Diatraea saccharalis ? A possible role for DsREase and other nucleases. Pesticide Biochemistry and Physiology, v. 186, p. 105166. https://doi.org/10.1016/j.pestbp.2022.105166 RIBEIRO, T.P.; Vasquez, D.D.N.; Macedo, L.L.P.; Lourenço-Tessutti, I.T.; Valença, D.C.; Oliveira-Neto, O.B.; Paes-de-Melo, B.; Rodrigues-Silva, P.L.; Firmino, A.A.P.; Basso, M.F.; Lins, C.B.J.; Neves, M.R.; Moura, S.M.; Tripode, B.M.D.; Miranda, J.E.; Silva, M.C.M.; Grossi-de-Sa, M.F. (2022). Stabilized Double-Stranded RNA Strategy Improves Cotton Resistance to CBW (Anthonomus grandis ). International Journal of Molecular Sciences , v. 23, n. 13713. https://doi.org/10.3390/ijms232213713 RIBEIRO, D.G.; Mota, A.P.Z.; Santos, I.R.; Arraes, F.B.M.; Grynberg, P.; Fontes, W.; Castro, M.S.; Sousa, M.V.; Lisei-de-Sá, M.E.; Grossi-de-Sa, M.F. ; Franco, O.L.; Mehta, A. (2022). NBS-LRR-WRKY genes and protease inhibitors (PIs) seem essential for cowpea resistance to root-knot nematode. Journal of Proteomics , p. 104575. https://doi.org/10.1016/j.jprot.2022.104575 dos SANTOS, C., Carmo, L.S.T., Távora, F.T.P.K.; Lima, R.F.C.; Mendes, P.N.; Labuto, L.B.D.; de Sá, M.E.L.; Grossi-de-Sa, M.F.; Mehta, A. (2022). Overexpression of cotton genes GhDIR4 and GhPRXIIB in Arabidopsis thaliana improves plant resistance to root-knot nematode (Meloidogyne incognita ) infection. 3 Biotech , v. 12, p. 211. https://doi.org/10.1007/s13205-022-03282-4 TÁVORA, F.T.P.K.; Diniz, F.A.S.; Rêgo-Machado M.C.; Freitas, N.C.; Arraes, F.B.M.; Andrade, C.E.; Furtado, L.L.; Osiro, K.O.; Lima de Sousa, N.; Cardoso, T.B.; Henning, L.M.M.; Molinari, P.A.O.; Feingold, S.E.; Hunter, W.B.; Grossi de Sá, M.F. ; Kobayashi, A.K.; Nepomuceno, A.L.; Santiago, T.R.; Molinari, H.B.C. (2022). CRISPR/Cas- and topical RNAi-based technologies for crop management and improvement: reviewing the risk assessment and challenges towards a more sustainable agriculture. Frontiers in Bioengineering and Biotechnology , v. 10, p. 913728. https://doi.org/10.3389/fbioe.2022.913728 2021 ARAUJO SOUSA, B.; Nascimento Silva, O.; Farias Porto, W.; Lima Rocha, T.; Paulino Silva, L.; Ferreira Leal, A.P.; Buccini, D.F.; Oluwagbamigbe Fajemiroye, J.; de Araujo Caldas, R.; Franco, O.L.; Grossi-De-Sá, M.F. ; de La Fuente Nunez, C.; Moreno, S.E. (2021). Identification of the active principle conferring anti inflammatory and antinociceptive properties in bamboo plant. Molecules , v. 26, p. 3054. https://doi.org/10.3390/molecules26103054 ARRAES, F.B.M.; Martins-de-Sa, D.; Noriega Vasquez, D.D.; Melo, B.P.; Faheem, M.; de Macedo, L.L.P.; Morgante, C.V.; Barbosa, J.A.R.G.; Togawa, R.O.; Moreira, V.J.P.; Danchin, E.G.J.; Grossi-de-Sa, M.F. (2021). Dissecting protein domain variability in the core RNA interference machinery of five insect orders. RNA Biology , v. 18, p. 1653-1681. https://doi.org/10.1080/15476286.2020.1861816 BASSO, M.F.; Costa, J.A.; Ribeiro, T.P.; Arraes, F.B.M.; Lourenço-Tessutti, I.T.; Macedo, A.F.; Neves, M.R.; Nardeli, S.M.; Arge, L.W.; Perez, C.E.A.; Silva, P.L.R; De Macedo, L.L.P.; Lisei-de-Sa, M.E.; Amorim, R.M.A.; Pinto, E.R.C.; Silva, M.C.M.; Morgante, C.V.; Floh, E.I.S.; Alves-Ferreira, M.; Grossi-de-Sa, M.F. (2021). Overexpression of the CaHB12 transcription factor in cotton ( Gossypium hirsutum ) improves drought tolerance. Plant Physiology and Biochemistry , v. 165, p. 80-93. https://doi.org/10.1016/j.plaphy.2021.05.009 CABRAL, D.; Forero Ballesteros, H.; de Melo, B.P.; Lourenço-Tessutti, I.T.; Smões de Siqueira, K.M.; Obicci, L.; Grossi-de-Sa, M.F. ; Hemerly, A.S.; de Almeida Engler, J. (2021). The armadillo BTB protein ABAP1 is a crucial player in DNA replication and transcription of nematode-induced galls. Frontiers in Plant Science , v. 12, p. 636663. https://doi.org/10.3389/fpls.2021.636663 LISEI-DE-SÁ, M.E.; Rodrigues-Silva, P.L.; Morgante, C.V.; de Melo, B.P.; Lourenço-Tessutti, I.T.; Arraes, F.B.M.; Sousa, J.P.A.; Galbieri, R.; Amorim, R.M.S.; de Lins, C.B.J.; Macedo, L.L.P.; Moreira, V.J.; Ferreira, G.F.; Ribeiro, T.P.; Fragoso, R.R.; Silva, M.C.M.; de Almeida-Engler, J.; Grossi-de-Sa, M.F. (2021). Pyramiding dsRNAs increases phytonematode tolerance in cotton plants. Planta , v. 254, p. 121. https://doi.org/10.1007/s00425-021-03776-0 MENDES, R.A.G.; Basso, M.F.; Paes-de-Melo, B.; Ribeiro, T.P.; Lima, R.N.; Araujo, J.F.; Grossi-de-Sa, M.; Mattos, V.S.; Togawa, R.C.; Albuquerque, E.V.S.; Lisei-de-Sa, M.E.; Silva, M.C.M.; Macedo, L.L.P.; Fragoso, R.R.; Fernandez, D.; Vignols, F.; Grossi-de-Sa, M.F. (2021). The Mi-EFF1/Minc17998 effector interacts with the soybean GmHub6 protein to promote host plant parasitism by Meloidogyne incognita . Physiological and Molecular Plant Pathology , v. 114, p. 101630. https://doi.org/10.1016/j.pmpp.2021.101630 MENDES, R.A.G.; Basso, M.F.; Fernandes de Araújo, J.; Paes De Melo, B.; Lima, R.N.; Ribeiro, T.P.; da Silva Mattos, V.; Saliba Albuquerque, E.V.; Grossi-De-Sa, M.; Dessaune Tameirao, S.N.; da Rocha Fragoso, R.; Mattar da Silva, M.C.; Vignols, F.; Fernandez, D.; Grossi-De-Sa, M.F. (2021). Minc00344 and Mj-NULG1a effectors interact with GmHub10 protein to promote the soybean parasitism by Meloidogyne incognita and M. javanica. Experimental Parasitology , v. 229, p. 108153. https://doi.org/10.1016/j.exppara.2021.108153 MOREIRA-PINTO, C.E.; Ramos Coelho, R.; Borges Leite, A.G.; Amaral Silveira, D.; Aguiar Souza, D.; Biaggioni Lopes, R.; Macedo, L.L.P.; Mattar Silva, M.C.; Ribeiro, T.P.; Morgante, C.V.; Antonino, J.D.; Grossi-de-Sa, M.F. (2021). Increasing susceptibility to through-induced knockdown: a perspective to combine biocontrol and biotechnology. Pest Management Science , v. 77, p. ps.6430. https://doi.org/10.1002/ps.6430 MOREIRA-PINTO, C.E.; Coelho, R.R.; Leite, A.G.B.; Silveira, D.A.; Souza, D.A.; Lopes, R.B.; Macedo, L.L.P.; Silva, M.C.M.; Ribeiro, T.P.; Antonino, J.D.; Grossi-de-Sa, M.F. (2021). Increasing Anthonomus grandis susceptibility to Metarhizium anisopliae through RNAi-induced AgraRelish knockdown: a perspective to combine biocontrol and biotechnology. Pest Management Science , v. 77, p. 4054-4063. https://doi.org/10.1002/ps.6430 MOTA, A.P.Z.; Brasileiro, A.C.M.; Vidigal, B.; Oliveira, T.N.; da Cunha Quintana Martins, A.; Saraiva, M.A.P.; de Araújo, A.C.G.; Togawa, R.C.; Grossi-de-Sá, M.F. ; Guimaraes, P.M. (2021). Defining the combined stress response in wild Arachis . Scientific Reports , v. 11, p. 11097. https://doi.org/10.1038/s41598-021-90607-7 PAES DE MELO, B.; Lourenço-Tessutti, I.T.; Fraga, O.T.; Pinheiro, L.B.; de Jesus Lins, C.B.; Morgante, C.V.; Engler, J.A.; Reis, P.A.B.; Grossi-De-Sá, M.F. ; Fontes, E.P.B. (2021). Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses. Scientific Reports , v. 11, p. 11178. https://doi.org/10.1038/s41598-021-90767-6 PAES DE MELO, B.; Moura, S.M.; Morgante, C.V.; Pinheiro, D.H.; Alves, N.S.F.; Rodrigues-Silva, P.L.; Lourenço-Tessutti, I.T.; Andrade, R.V.; Fragoso, R.R.; Grossi-de-Sa, M.F. (2021). Regulated promoters applied to plant engineering: an insight over promising soybean promoters under biotic stress and their cis-elements. 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Plant Biotechnology Journal , v. 15(8), p. 997-1009. https://doi.org/10.1111/pbi.12694 2016 OLIVEIRA, R.S.; Oliveira-Neto, O.B.; Moura, H.F.; Macedo, L.L.P.; Arraes, F.B.M.; Lucena, W.A.; Lourenço-Tessutti, I.T.; Barbosa, A.A.D.; Silva, M.C.M.; Grossi-de-Sa, M.F. (2016). Transgenic cotton plants expressing Cry1Ia12 toxin confer resistance to fall armyworm (Spodoptera frugiperda ) and cotton boll weevil (Anthonomus grandis ). Frontiers in Plant Science , v. 7, p. 165. https://doi.org/10.3389/fpls.2016.00165 LACERDA, A.F.; Pelegrini, P.B.; Oliveira, D.M.; Vasconcelos, E.A.; Grossi-de-Sa, M.F. (2016). Anti-parasitic peptides from arthropods and their application in drug therapy. Frontiers in Microbiology , v. 7, p. 91. https://doi.org/10.3389/fmicb.2016.00091 COELHO, R.R.; Antonino, J.D.; Firmino, A.A.P.; Macedo, L.L.P.; Fonseca, F.C.A.; Terra, W.R.; Engler, G.; Almeida-Engler, J.; Silva, M.C.M.; Grossi-de-Sa, M.F . (2016). Vitellogenin knockdown strongly affects cotton boll weevil egg viability but not the number of eggs laid by females. Meta Gene , v. 9, p. 173-80. https://doi.org/10.1016/j.mgene.2016.06.005 GUIMARAES-DIAS, F.; Neves-Borges, A.C.; Conforte, A.J.; Giovanella-Kampmann, L.; Ferreira, A.V.J.; Amorim, R.M.S.; Beneventi, M.A.; Lisei-de-Sa, M.E.; Mesquita, R.O.; Rodrigues, F.A.; Nepomuceno, A.L.; Romano, E.; Loureiro, M.E.; Grossi-de-Sa, M.F. ; Alves-Ferreira, M. (2016). Differential impact of acclimation and acute water deprivation in the expression of key transcription factors in soybean roots. Plant Molecular Biology Reporter , v. 34, p. 1167-1180. https://doi.org/10.1007/s11105-016-0993-z VILLETH, G.R.; Carmo, L.S.; Silva, L.P.; Santos, M.F.; Oliveira-Neto, O.B.; Grossi-de-Sa, M.F. ; Ribeiro, I.S.; Dessaune, S.N.; Fragoso, R.R.; Franco, O.L.; Mehta, A. (2016). Identification of proteins in susceptible and resistant Brassica oleracea responsive to Xanthomonas campestris pv. campestris infection. Journal Proteomics , v. 143, p. 278-285. https://doi.org/10.1016/j.jprot.2016.01.014 PATENTS HEMERLY, A.S.; Cavalcanti, P.F.; Gong, P.; Nelissen, H.; Inze, D.; Grossi-de-Sa, M.F. ; Basso, M.F.; Morgante, C.V.; Lisei-de-Sa, M.E. Method for promoting an increase in plant biomass, productivity, and drought resistance. US 2020/0347399 A1 . Deposit: May 4, 2020. Publication: November 5, 2020. GROSSI-DE-SA, M.F. ; Silva, M.C.M.; Fonseca, F.C.A.; Macedo, L.L.P.; Lourenco, I.T.; Albuquerque, E.V.S. Aparato e método de criação de larvas de insetos em laboratório. BR 1020130331120 B1 . Deposit: December 20, 2013. Patent granted: November 24, 2020. GROSSI-DE-SA, M.F. ; Oliveira, G.R.; Silva, M.C.M.; Rocha, T.L.; Magalhaes, M.T.Q. Molécula de ácido nucléico isolada, construção gênica, vetor, célula transgênica, método para obtenção de uma célula e de uma planta transgênica. PI 0906128-2 B1 . Deposit: July 24, 2009. Patent granted: March 17, 2020. GROSSI-DE-SA, M.F. ; Macedo, L.L.P.; Silva, M.C.M.; Almeida-Garcia, R.; Silva, L.P.; Vila, A. Pesticide. WO 2020/007450 A1 . Deposit: July 7, 2018, Publication: January 9, 2020. GROSSI-DE-SA, M.F. ; Macedo, L.L.P.; Pinto, C.E.M.; Leite, A.G.B.; Silva, M.C.M.; Lourenço-Tessutti, I.T.; Morgante, C.V. Método para produzir planta resistente a inseto praga e moléculas de ácido nucleicos utilizado para obtenção de tal planta através de dsRNAs relacionados a ecdise. PI 102020004312-9 . Deposit: March 3, 2020. ROCHA, T.L.; Evaristo, R.G.S.; Grossi-de-Sa, M.F. ; Silva, M.C.M.; Polez, V.L.P.; Roessner, U.; Bacic, T. Nematotoxic composition of synergistic effect, use of a nematotoxic composition of synergistic effect. US 9750247 B2, CN 105007725 B . Deposit: December 26, 2013 (US and CH). Patent granted: September 5, 2017 (US) and April 4, 2019 (CH). MACEDO, L.L.P.; Grossi-de-Sa, M.F. ; Silva, M.C.M.; Almeida-Garcia, R.; Mendes, R.A.G.; Albuquerque, E.V.S. Increasing the efficiency of supression of gene expression by means of the use of RNA molecules with a stabilized structure. BR 10201700690-4 A2, WO 2018/184083 A1. Deposit: April 4, 2017 (BR) and March 29, 2018 (US). Publication: October 30, 2018 (BR) and November 11, 2018 (US). GROSSI-DE-SA, M.F. ; Silva, M.C.M.; Del-Sarto, R.P.; Rocha, T.L. Mutantes de inibidores de alfa amilases isolados de Phaseolus vulgaris com propriedades de controlar insetos-praga, composições contendo tais mutantes e métodos de obtenção dos mesmos e de linhagens transgênicas. PI 1102841-6 . Deposit: June 8, 2011. Patent granted: November 26, 2018. GROSSI-DE-SA, M.F. ; Coelho, R.R.; Firmino, A.A.P.; Macedo, L.L.P; Silva, M.C.M.; Lourenço-Tessutti, I.T. Método e composições para controle de insetos-praga em plantas por meio do silenciamento de genes da família da quintina sintase e da vitelogenina bem como alternativamente pela expressão do gene de uma toxina Cry. BR 102013032649-6 A2 . Deposit: December 18, 2013. Publication: February 10, 2016. SOUZA, D.S.L.; Silva, M.C.M.; Grossi-de-Sa, M.F. ; Evaristo, R.G.S.; Rocha, T.L.; Bacic, T.; Roessner, U.; Polez, V.L.P. Composição nematotóxica de efeito sinérgico, uso de composição nematotóxica de efeito sinérgico. BR 10201203314-6 A2 . Deposit: December 26, 2012. Patent granted: August 26, 2014. MESSENBERG, P.G.; Grossi-de-Sa, M.F. Métodos para detecção e identificação de Curtobacterium flaccumfaciens pv flaccumfaciens em uma amostra biológica e método de certificação de sementes. PI 9806477-0 . Deposit: June 23, 1998. Patent granted: April 29, 2014. GROSSI-DE-SA, M.F. ; Silva, M.C.M.; Macedo, L.L.P.; Firmino, A.A.P.; Coelho, R.R.; Lourenço-Tessuti, I.T. Método e composições para controle genético de insetos-praga em plantas de algodão através do silenciamento de genes de quitina sintases. BR 102012033539-5 A2 , US 10182571 B2 and WO 2014/100879 A2 . Deposit: December 28, 2012 (BR), December 27, 2013 (US) and June 27, 2013 (WO). Publication: August 18, 2015 (BR), January 22, 2019 (US) and January 3, 2014 (WO). GROSSI-DE-SA, M.F. ; Silva, M.C.M.; Gomes-Jr, J.E.; Lourenço-Tessuti, I.T.; Macedo, L.P.P.; Lucena, W.A.; Fonseca, F.C.A. Moléculas variantes sintéticas de toxinas Cry1Ia12 com propriedades de controlar insetos-praga, composições contendo tais mutantes e método de utilização dos mesmos. WO 2014-100880 A3 . Deposit: 02/12/2012. Publication: July 3, 2014. GROSSI-DE-SA, M.F. ; Romano, E.; Fragoso, R.R.; Silva, M.C.M.; Ferreira, A.V.J.; (2013). Cassete de expressão para indução de resistência a múltiplas espécies de nematóides em plantas, métodos e plantas que o utilizam. WO 2014/197951 A2 . Deposit: June 11, 2013. 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Find out more about the project, the specific objectives and the institutions involved. Acerca de About the Project Biological systems research focusing on elucidating plant stress mechanisms and signaling pathways is important for developing resistant cultivars in a climate change scenario. Pathogen attacks and abiotic stresses such as water deficit are essential during plant development, and the identification of genes and molecules, which regulate resistance responses, are critical to develop crop cultivars capable of doing in the face of these stresses. In association with abiotic stresses, plants must also defend themselves against pest’s attacks, including fungi, bacteria, insect pests, and nematodes. Insect-pests comprise the most serious factor affecting productivity losses in the Brazilian agribusiness. Losses in soybean, cotton, and corn caused by the caterpillars Helicoverpa armigera , Spodoptera frugiperda , and phytonematodes constitute the most critical phytosanitary problem in terms of competitiveness for the commodities production and exportation. Faced with scenario cases involving plant resistance breakdown, environmental association of biotic and abiotic stresses, fewer characterized and introgressed genes, and higher complexity of plant responses have been reported. All these reports demonstrate the importance of the research towards the identification of genes/molecules involved in pest resistance and water deficit responses. In this context, the National Institute of Science and Technology, PlantStress Biotech INCT, integrates various Brazilian research groups and international partners, experts in plant physiology, transcriptomic, epigenetic, proteomic, bioinformatic and functional genomics analyses. The integrative research group represents a multidisciplinary and multi-institutional network with national and international excellence to generate innovative biotechnologies applied to corn, soybean and cotton focused on the tolerance to deficit hydric and pest control (Meloidogyne spp, H. armigera and S. frugiperda ). The project includes bioprospection, isolation, characterization, and functional validation of genes/molecules involved in plant pest resistance and drought tolerance. The PlantStress Biotech INCT action comprises: Prospection for genes and molecules involved in resistance/tolerance to specific and combined stresses in native plants and resistant/tolerant contrasting genotypes, through the elucidation of the stress mechanisms and signaling pathways; Prospection for genes and molecules involved in the defense and parasitism of target pests; Search for promoters responsive to pests and water deficit, and small RNAs involved in the regulation of target genes; Use different strategies (gene/molecules overexpression or gene silencing) for biological functional validation in model systems; Developing of new nanoencapsulated insecticides/nematicides from validated molecules; Developing of biotechnological products (concept proof) in GM crop plants (cotton, soybean, and maize); Evaluation of developed GM crop plants in simulated field experiments. The biotech assets generated will be applied to the development of biotechnology products, including nanobioinsecticides and genetically modified crops (soybean, maize, and cotton) against biotic and abiotic stresses. In addition to the biotech assets obtained and characterized in this project, an understanding of molecular and physiological factors related to drought tolerance and their interaction with pests and other environmental stresses will contribute to prevent, unravel and adapt to climatic changes. Alongside its scientific agenda, the PlantStress Biotech INCT group, formed by researchers from five Embrapa units and five Federal Universities (UnB, UFRJ, UFRGS, UFC, and UFPel), and collaborators from the public and private sectors, concentrate their efforts on training students and professionals in different and update approaches involving biotechnology, genomics, and bioinformatics. Actions will also strengthen joint action and the internationalization of undergraduate and graduate training engaged in PlantStress Biotech INCT research, and to increase the visibility and international insertion of its teams. The PlantStress Biotech INCT network focuses not only on the generation of biotech assets applied to drought and pests in soybean, cotton, and maize but also on other important agronomic traits (seed and fruit quality, an increase of biomass and nutritional value, among other) and in other crops important for Brazilian agribusiness.