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Learn more about AL05, its main goals within the project and meet the laboratory's work team. AL 05 - Plant-Pest Interaction Laboratory Activities - PlantStress Biotech INCT Sequencing transcriptomes associated with the response to drought of native species in Brazil (wild peanuts, pitangueira, Clúsia; and cashew) by large-scale sequencing. Identify and select in silico candidate genes related to drought tolerance of native species (wild peanuts, pitangueira, Clúsia, and cashew). Identify InDels and SNPs in candidate genes of native species associated with the drought response (wild peanuts, pitangueira, and cashew). Validate in vitro the expression profile of candidate genes for tolerance to drought obtained from native species in Brazil (wild peanuts, pitangueira, Clúsia, and cashew). Select vital genes/molecules for gall nematodes (Meloidogyne spp.) by analyzing their genome. Select potential genes involved in resistance from contrasting genotypes (peanuts, soybean, rice, cotton and coffee). Integrate legume transcriptome data (beans, soybean, and peanuts) submitted to water deficit, generated by mass sequencing (Illumina – HiSeq) in previous projects. Integrate transcriptome data from resistant genotypes (beans, soybean, rice, coffee, and peanuts) infected by nematodes, generated by mass sequencing (Illumina – HiSeq) in previous projects. Sequencing and integrating transcriptome of drought tolerant genotypes of Musa spp., Arachis spp., and cowpea subjected to water deficit combined with biotic stress (Meloidogyne spp. or Mycosphaerella ) in bioassays. Validate the expression of key genes in the metabolic pathways of plants' response to combined stresses (biotic-biotic; biotic-biotic; abiotic-biotic) by qRT-PCR. Sequencing on the Illumina platform a fraction of small RNAs, and their target mRNAs, and circular RNAs of plants (Arachis ; Musa ; soybean; pitangueira; cashew tree) subjected to biotic and/or abiotic stresses. Analyze sequencing data to check methylation status of gene promoters in the metabolic pathways of interest. Validate the function of plant genes potentially involved in the mechanisms of drought tolerance in Arabidopsis , rice or sepia plants via overexpression or silencing strategies. Validate the function of plant genes potentially involved in nematode resistance mechanisms via overexpression or silencing strategies. Validate the function of nematode genes potentially involved in the parasitism mechanisms, via gene silencing strategies in model systems. Assess the potential of biotechnological assets generated for the purpose of intellectual protection. Patent the use of genes and gene elements validated during the project Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Laboratory Description The Plant-Pest Interaction Laboratory conducts studies on the interaction of plants with biotic and abiotic stresses, aiming at elucidating the changes in the cellular, biochemical, physiological and molecular machinery of plants, which occur in response to different stresses, combined or not. In addition to prospecting and identifying genes, regulatory sequences and molecules involved in plant tolerance/resistance responses to one or more stresses, research group performs the validation of function of these assets in model plants and methods are developed and improved for the validation of these assets in target plants, which will enable the development of cultivars more adapted to different environmental conditions. Research Lines Prospecting for genes/molecules or peptides of interest for the control of drought and pests in wild peanut germplasm (Arachis spp.). Prospecting of target molecules in phytoparasitic nematodes (Meloidogyne spp.) for pest control. Prospecting for small RNAs in plant genotypes that are resistant/tolerant to pests and drought that may be involved in these stresses. Prospecting for molecules and peptides that are efficient in controlling pests and tolerating water deficit simultaneously (cross-stress). Vallidation of assets due to their overexpression or gene silencing in model plants for analysis and validation of their function. Our Team Patrícia Messemberg Guimarães Team Leader Graduated in Agronomy from the University of Brasília (1985), master's degree in Phytopathology from the University of Brasília (1987) and doctorate in Molecular Biology - University of London (1997). She did post-doctorate in plant genomics at CIRAD (France) in 2006. She is currently a collaborator at the University of Brasilia (Unb) and Catholic University of Brasilia (UCB) and researcher at the Brazilian Agricultural Research Corporation since 1989. The main research areas include Biochemistry, Molecular Biology, Plant-pathogen interaction, and works mainly on the following themes: structural and functional genomics of legumes, Arachis genomics, plant resistance, plant tolerance to water stress, genetic maps, and molecular characterization of plants. She is coordinator of several national and international projects in the field of genetics and genomics of legumes and plant-pest interaction. Ana Cristina Miranda Brasileiro Graduated in Forestry Engineering from the University of Brasília (1986), Master in Molecular and Plant Cell Biology - Universite de Paris XI (Paris-Sud) (1988) and PhD in Molecular and Plant Cell Biology - Universite de Paris XI (Paris-Sud ) (1992). He is currently a researcher at the Brazilian Agricultural Research Corporation. From 2002 to 2006 she worked as a researcher at Labex-Europa at Cirad (France). He has experience in the field of Genetics, with an emphasis on Plant Genetics, working mainly on the following themes: genetic transformation, plant biotechnology, Agrobacterium biology, gene expression and genomics. Since 2006, she has been coordinator in Brazil of the International Consortium in Advanced Biology (CIBA), an initiative of Agropolis (France) and Embrapa (Brazil), whose objective is to create and consolidate an efficient strategy of international scientific and technical cooperation, to study and explore the diversity in genetic resources of plants and to identify important genes and characteristics essential for genetic improvement programs in Tropical and Mediterranean agriculture. From 2008 to 2014 he participated as an external member of the Scientific Council of the Department of Biological Systems (BIOS) of Cirad/France and since 2016 he has been an external member of the Scientific and Strategic Council of Cirad/France. Ana Claudia Guerra de Araújo Researcher Ana Claudia Guerra de Araujo has a degree in Biology from the University of Brasília (1987), a PhD in Biological Sciences (Biophysics) from the Federal University of Rio de Janeiro (1994). Since 1994 she is a Researcher at Embrapa, at Cenargen, where she has been working with the interface between cellular and plant molecular biology at the Microscopy Laboratory. He has a post-doctorate in Australia (CSIRO, 2001) where he worked with molecular and cellular techniques in plant reproduction and in England (University of Leicester, 2011), where he worked with plant molecular cytogenetics using the microscopy tool. At Embrapa, he develops research in the area of plant reproduction, through studies of morphology, cell biology and ultrastructure of developmental biology, involving techniques of cytochemistry, immunocytochemistry, cytogenetics, in situ hybridization associated with microscopy. It is also involved in studies on the plant-pathogen interaction, responses to biotic and abiotic stresses and quality in Arachis, determining factors for the success of a productive and sustainable agriculture. André Southernman Teixeira Irsigler Graduated in Biological Sciences (2000), Master (2002) and Doctorate (2007) in Genetics and Breeding from Universidade Federal de Viçosa, with a sandwich period at North Carolina State University, and Post-doctorate at Florida State University. He is a researcher at Embrapa Genetic Resources and Biotechnology, and is involved in the areas of regulation of gene expression and developmental biology. Contact Patricia Messenberg Guimarães EMBRAPA Genetic Resources and Biotechnology W5 Norte Avenue (end) - P.O. Box 02372 - Postal Code 70770-917 - Brasília, DF - Brazil E-mail: patricia.guimaraes@embrapa.br Phone:+55 61 3448-4787

Here you can find all registered patents. Results of works carried out by the INCT PlantStress Biotech network. Publications Patents RELEVANT PATENTS (FROM 2017) Application Patents / Grant Patents / Licensed Patents ALVES-FERREIRA, M, Conforte, AJ, Dias, FG, Malato, MB, Grossi-de-Sa, MF, Nepomuceno, AL (2018). Compositions and Methods for Modifying Gene Expression Using Water Deficit-Inducible Soybean Promoter in Plants. BR1020180037692. Instituição de registro: INPI - Instituto Nacional da Propriedade Industrial. BRASILEIRO, ACM, Guimaraes, PM, Araújo, ACG, Lacorte, CC (2018) Método para Produção de Plantas com Tolerância a Estresse Abiótico e com Resistência a Pragas. BR102018003666-1. Instituição de registro: INPI - Instituto Nacional da Propriedade Industrial. FREITAS-SILVA, MV, Barreto-Bergter, E, Silva-Neto MAC, Montebianco, CB, Bernardino, MC, Mattos, B,Carvalho, SS (2017). Composição Praguicida, Uso, Método de Controle de Patógenos de Plantas, Método de Controle de Propagação de Insetos e Processo de Obtenção de Proteínas de Fungos. BR10201700204. Instituição de registro: INPI – Instituto Nacional da Propriedade Industrial. FREITAS-SILVA, MV, Côrrea, RL, Vidal, MS, Barroso, PAV. Teste Molecular Voltado para a Identificação e o Diagnóstico In Vitro do Vírus Responsável pela Doença Azul do Algodoeiro (CLRDV). PI06001513. Instituição de registro: INPI - Instituto Nacional da Propriedade Industrial. Concessão: 15/08/2017 . GUIMARÃES, LM, Batista, JAN, Viana, AMB, Fragoso, RR, Silva, MCM, Grossi-de-Sa, MF. Composições e Métodos para Modificar a Expressão de Genes Usando o Promotor do Gene da Proteína de Conjugação Ubiquitina de Plantas de Soja. INPI -Data do Depósito: 05/02/2007. CARTA PATENTE Nº PI0701172-5, concessão: 26/11/2019 . GROSSI-DE-SA, MF, Silva, MCM, Del Sarto, RP, Rocha, TL. Mutantes de Inibidores de Alfa Amilases Isolados de Phaseolus vulgaris com propriedades de Controlar Insetos-Praga, Composições Contendo Tais Mutantes e Métodos de Obtenção dos Mesmos e de Linhagens Transgênicas. INPI-Brazil: PI1102841-6, Deposito: 08/06/2011. CARTA PATENTE Nº PI 1102841-6. Concessão: 21/11/2018 . GROSSI-DE-SA, M.F.; Grossi-de-Sa, M.F; Silva, M. C. M.; Fonseca, F. C. A.; Macedo, L. L. P.; Lourenco, I. T.; Albuquerque, E. V. S. (2013). Aparato e método de criação de larvas de insetos em laboratório. Patent application, BR1020130331120, 20/12/2013, INPI-Brazil. CARTA PATENTE Nº BR102013033112-0. Concessão: 24/11/2020 . GROSSI-DE-SA, MF, Guimarães, LM, Batista, JAN, Viana, AAB, Fragoso, RR, Rocha, T (2007). Composições e métodos para modificar a expressão de genes usando o promotor do gene da proteína de Conjugação à ubiquitina de plantas de Algodão. 2007, Brasil. INPI – Brazil, PI07012306, Deposito: 05/02/2007, Publication Date: 23/09/2008. CARTA PATENTE Nº PI 0701230-6 B1. Concessão: 26/06/2018 . GROSSI-DE-SA, MF, Macedo, LLP. Silva, MCM, Almeida Garcia, R, Silva, LP, Vila A (2018). Pesticide, European Patent Office. WO2020007450, PCT/EP2018/067922. Patent Application: 03/07/2018. GROSSI-DE-SA, MF, Brasileiro, ACM, Guimarães, PM, Morgante, CV, Lourenço-Tessutti, IT, Arraes, FBM, Pereira, BM (2022). High Throughput Soybean Root Disease Screen. US Patent Application WO2024073346. filled Patent: 26/09/2022. GROSSI-DE-SA, MF, Macedo, LLP, Pinto, CLM, Leite, AGB, Silva, MCM, Lourenço-Tessutti, IT, Morgante, CV (2020). Método para Produzir Planta Resistente a Inseto Praga e Moléculas de Ácido Nucleico Utilizadas para Obtenção de Tal Planta Através de dsRNAs Relacionados a Ecdise. PI1020200043129 A2. Instituição de registro: INPI - Instituto Nacional da Propriedade Industrial. GROSSI-DE-SA, MF, Macedo, LLP, Salles-Filho, ALO, Silva, MCM, Lourenço-Tessuti, IT, Morgante, CV (2025). Biocomplexos para modificação genética in planta. 2025, Brasil. Patente: Privilégio de Inovação. Número do registro: BR10202500402. INPI - Instituto Nacional da Propriedade Industrial. Depósito: 28/02/2025. GROSSI-DE-SA, MF, Silva, MCM, Macedo, LLP, Lourenço-Tessutti, IT, Morgante, CV, Leite, AGB (2021). Silenciamento do gene para a proteína caderina para aplicação no controle do inseto-praga, Anthonomus grandis, via RNA interferente. 2021, Brasil.Patente: Privilégio de Inovação. Número do registro: BR1020210115300, Instituição de registro: INPI - Instituto Nacional da Propriedade Industrial. Depósito: 14/06/2021. Hemerly, AS, Cavalcanti, PF, Gong P, Nelissen H, Inze D, Grossi-de-Sa, MF, Basso, MF, Morgante, CV, Lisei-de-Sa, ME (2020). Method to increase plant biomass, plant yield and plant drought tolerance. Continuation in part (CIP) patent application US†20160177327†A1. United States Patent and Trademark Office Patent Filling: 04/05/2020. Patent Grant: 13/12/2022. Licensed Patent: 2023 – INARI SEEDesign Company. MACEDO, LLP, Grossi-de-Sa, MF, SILVA, MCM, GARCIA, RAG, Freire, EVSA (2017). Aumento da Eficácia de Supressão de Expressão de Genes por Meio do Uso de Molécula de RNA com Estrutura Estabilizada. BR1020170069044. Instituição de registro: INPI - Instituto Nacional da Propriedade Industrial. REMER, R. A.; Margis, R.; Lima, M. C.; Coronha, L. M.; Alves-Ferreira. Produto Farmacêutico e Processo para sua Produção. PI03051978. Instituição de registro: INPI – Instituto Nacional da Propriedade Industrial. Depósito: 13/11/2003; Concessão: 02/04/2019 . ROCHA-LIMA, T, Evaristo, RGS, Grossi de Sa, MF, Silva, MCM, Polez, VLP, ROESSNER, U, Bacic, T. (2017). Nematotoxic composition of synergistic effect. use of a nematotoxic composition of synergistic effect. United States Patent and Trademark Office. US9750247B2. Patent Application: 26/12/2012; PCT Application: 26/12/2013; Patent Grant: 05/09/2017 . ROCHA-LIMA, T, Grossi-de-Sa, MF, Silva, MCM, Polez, VP, Evaristo, RGS, Roessner, Um, Bacic, T (2013). Composição nematotoxica de efeito sinergico, uso de composição nematotóxica de efeito sinérgico. 2013, Brasil.Patente: Privilégio de Inovação. Número do registro: BR0020130095109, Instituição de registro: INPI - Instituto Nacional da Propriedade Industrial. Depósito: 26/12/2013; Depósito PCT: 26/12/2013; Concessão: 03/11/2021 . GROSSI-DE-SA, MF, Silva, MCM, Gomes Jr, JE, Lourenco, IT, Macedo LLP, Lucena WA, Fonseca, FCA (2013). Moléculas variantes sintéticos de toxinas Cry1IA12 com propriedades de controlar insetos-praga, composições contendo tais mutantes e método de utilização dos mesmos: BR102012033542-5 A2, concessão: 24/08/2021 . Synthetic variant molecules of toxins Cry1IA12 with properties to control pest insects, compositions containing such mutants and method of use of the same: AR094338A1, concessão: 01/12/2023 . Instituição de registro: WIPO - World Intellectual Property Organization. CARNEIRO, NP, Almeida-Carneiro, A, Hercos Valicente, F, Martins Mendes S, Alvs, MC, Neto Parentoni, S (2024). Method for the control of populations of insect pests resistant to the VIP3AA Protein. INPI BR102023004777A2 (14/03/2023), PCT PCT/BR2024/050091 (13/03/2024), WO2024187259 (18/09/2024). Carneiro, NP, Valicente, FH, Carneiro, AA, Noda, RW, Meire,CA, Barros, BA (2018). Molécula de ácido nucleico cry1DA com códons otimizados, construções de DNA, vetor, célula hospedeira, célula vegetal, planta transgênica, métodos de transformação e de controle de pragas (Pedido de patente BR1020180092638. Instituto Nacional da Propriedade Industrial – INPI.

Seminars e Lectures Plant and Animal Genome Conference 2023 (PAG 30) 13 a 18 de janeiro de 2023 – San Diego/EUA Integrated Omics Data Unravel Soybean Mechanisms Associated with Root-Knot Nematode Tolerance 53º Congresso Brasileiro de Fitopatologia 7 a 10 de agosto de 2023 – Brasília/DF RNAi-mediated parasitism gene silencing as a new source of resistance against the root-knot nematode International Congress of Plant Pathology 2023 20 a 25 de agosto de 2023 – Lyon/França RNAi-mediated parasitism gene silencing as source of crop protection resistance to Meloidogyne incognita 68º Congresso Brasileiro de Genética 12 a 15 de setembro de 2023 – Ouro Preto/MG RNAi technology: Developing RNAi-based solutions for crop plants Simpósio e Diplomação de Novos Membros Afiliados da ABC 2023-2027 – Região Norte 27 de outubro de 2023 – Palmas/TO Palestra Magna – RNA Technology: challenges and developing RNAi-based solutions for crop plants (From Bench to Market: developed biotech products) VIII Simpósio Brasileiro de Genética Molecular de Plantas 30 de maio a 2 de junho de 2023 – Florianópolis/SC Organização da sessão (Chair) Applied Plant Biotechnology International Plant & Animal Genome 31 - PAG 2024 San Diego on January 12-17, 2024- San Diego, CA, USA Palestra: RNAi-mediated down-regulation of the endogenous aip10 genes in transgenic cotton (Gossypium hirsutum ) events improves earliness, productivity, and drought tolerance XXIX Congresso Brasileiro de Entomologia e XIII CLE RNAi approach for insect pest control: Advances, applications, and Challenges 22 a 26 de setembro de 2024- Uberlandia – MG Palestra: RNAi approach for insect pest control: Advances, applications, and Challenges International Plant Molecular Biology (IPMB) 24-28 de junho de 2024 – Queensland- Australia Palestra: RNAi for insect pest control: Transgenic and topical approaches AgroBrasilia 2024 20-24 de maio de 2024 Brasilia-DF-Brasil Palestra: Controle Biológico de Pragas e Doenças na sua lavoura: Tecnologia de interferência de RNA (RNAi) para melhoramento de plantas e controle de pragas ICE2024 Kyoto- XXVII International Congress of Entomology 25-30 de Agosto de 2024 Palestra: RNAi and Bt approaches for insect pest control: advances, applications, and challenges International Plant & Animal Genome 32 - PAG 2025 San Diego on January 10-15, 2025 - San Diego, CA, USA Palestra: An Ex Vitro Soybean Hairy Root System for Screening Target Molecules Applied in Biotech Approaches

Learn more about AL15, its main goals within the project and meet the laboratory's work team. AL 16 - Biotechnological Applications of Microorganisms Laboratory Description - PlantStress Biotech INCT Virus:plant interaction and the identification of viruses associated with cotton diseases through sequencing and characterization of new viruses, development of molecular diagnostic tests, transcriptome and miRNA profile during viral infection. The main lines are the characterization of the molecular mechanism of resistance to CBD; the use of fungal molecules as inducers of protection against viral diseases; and the expression of SARS-CoV-2 antigens in plants. Research Lines Cell Biology, Biochemistry and Genetics of Microorganisms Biotechnological Applications of Microorganisms and their Products Microorganisms/Virus-Host Interactions Our Team Maite Vaslin de Freitas Silva Team Leader Associate Professor IV, member of PPG-Micro and PPG-PBV at UFRJ. Director of the Brazilian Society of Virology. Master's and PhD in Genetics from UFRJ in molecular biology of plants. Postdoc at IB UFRJ studying RNAi in antiviral defense. Visiting professor IB UFRJ 199-2001 and permanent professor at IMPG since 2002. I coordinate the Plant Molecular Virology Laboratory, whose lines are: the study of the virus: plant interaction and the identification of viruses associated with cotton diseases through sequencing and characterization of new viruses, development of molecular diagnostic tests, transcriptome and miRNA profile during viral infection. Today the main lines are the characterization of the molecular mechanism of resistance to CBD; the use of fungal molecules as inducers of protection against viral diseases; and the expression of SARS-CoV-2 antigens in plants. The group has 2 patents filed and 1 granted and is encouraging the birth of startups based on the research developed. Contact Maite Vaslin de Freitas Silva Federal University of Rio de Janeiro - UFRJ Department of Virology – Paulo de Góes Institute of Microbiology – UFRJ E-mail: maite@micro.ufrj.br Phone:+55 21 2560 8344

Learn more about AL01, its main goals within the project and meet the laboratory's work team. AL 01 - Genomics and Proteomics Laboratory Activities - PlantStress Biotech INCT Integration data from monocotyledon transcriptome (rice, corn, wheat, Musa and sorghum) submitted to water deficit, generated by mass sequencing (Illumina - HiSeq) in previous projects. Integration of transcriptome data from drought-tolerant genotypes of Musa spp., Arachis spp. and cowpea subjected to water deficit combined with biotic stress (Meloidogyne spp. or Mycosphaerella ) in bioassays. Integration and sequencing of transcripts of fungus-tolerant genotypes in environments subjected to multiple stresses (Musa X Mycosphaerella musicola and Fusarium oxysporun ). Validation of key genes expression in the metabolic pathways of plants' response to combined stresses (biotic-biotic; biotic-biotic; abiotic-biotic) by qRT-PCR. Small RNAs, mRNAs and circular plant RNAs sequencing from Arachis , Musa , soybean, pitangueira, and cashew, previously subjected to biotic and/or abiotic stresses, using the Illumina platform. Validation of plant genes function potentially involved in drought tolerance mechanisms in Arabidopsis , rice or Setaria plants by overexpression or silencing strategies. Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Laboratory Description The Genomics and Proteomics Laboratory studies the prospection and identification of new genes, regulatory sequences and molecules involved with the resistance/tolerance responses of plants to biotic and abiotic stresses, aiming at the elucidation of the molecular mechanisms of plants that occur in response to different stresses, combined or not. The research group also performs fucntion validation of these assets in model plants. Research Lines Prospecting genes/molecules of interest for pest and drought control in wild germplasm of Musa spp. Prospecting target molecules in phytoparasitic nematodes (Meloidogyne spp.) and phytopathogenic fungi (Pseudocercospora spp.) for pest control. Prospecting for small RNAs in plant genotypes that are resistant/tolerant to pests and drought that may be involved in responding to these stresses. Prospecting for genes/molecules that are efficient in pest control and tolerance to water deficit simultaneously (cross-stress). Validation of prospective innovation assets by their overexpression or gene silencing in model plants for analysis and validation of their function. Robert Neil Gerard Miller Team Leader Graduated in Biological Sciences - Manchester Metropolitan University, UK (1990), Master in Plant Protection - University Of Bath, UK (1991) and PhD in Molecular Biology and Phytopathology - University of Reading, UK (1995). He is currently Associate Professor I at the University of Brasilia (Campus Darcy Ribeiro, Department of Cellular Biology), supervising the Graduate Programs in Molecular Biology, Phytopathology and Microbial Biology. He worked between 2014 and 2016 as Coordinator of the Graduate Program in Molecular Biology (CAPES concept 6). He works as editor for the journals Annals of Botany and Tropical Plant Pathology, he is coordinator of national and international projects, mainly in the following themes: functional genomics of plants and microorganisms; search for genes for resistance to biotic stress in plants; and characterization of phytopathogenic and mycotoxigenic fungi. Contact Robert Neil Gerard Miller Microbiology Laboratory: Plant-Prague Interaction, Bloco I-1-35/8, Institute of Biological Sciences, Department of Cell Biology, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Asa Norte, Postal Code 70910-900, Brasília, DF, Brazil.

Learn more about AL07, its main goals within the project and meet the laboratory's work team. AL 07 - Insects and Nematodes: Breeding and Bioassays Laboratory Activities - PlantStress Biotech INCT Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Leonardo Lima Pepino de Macedo Team Leader Dr. Leonardo is undergraduated in Biological Sciences from the Federal University of Rio Grande do Norte (2005). He holds a master's degree in Biochemistry from the Federal University of Rio Grande do Norte (2007), and a doctorate's degree in Genomic Science and Biotechnology from the Catholic University of Brasília (2012). He has experience in Biochemistry and Molecular Biology, with expertise in the following topics: cloning and expression of proteins in heterologous systems; bioprospecting of proteins with entomotoxic activity (vicillins, lectins, proteinase inhibitors and Cry toxins) aiming at the control of dipterous, lepidopteran and coleopteran insects; and development of gene silencing strategies via interfering RNA (RNAi) for the control of insect pests. Contact Leonardo Lima Pepino de Macedo EMBRAPA Genetic Resources and Biotechnology W5 Norte Avenue (end) - P.O. Box 02372 - Postal Code 70770-917 - Brasília, DF - Brazil E-mail: leonardo.lima@embrapa.br Phone number:+55 61 3448-4705

Find here the events organized by INCT PlantStress Biotech and participate! Events Organization Workshops with the INCT-PlantStress-Biotech team , held in February/2022. Member of international committee XXVIII Plant and Animal Genome Conference , San Diego, CA, EUA, Antônio C. de Oliveira, held from 11 to 18 January 2020. Member of international committee ISRFG 2019 , Taipei, Taiwan, Antônio C. de Oliveira, held from 4 to 6 November 2019. Workshops with the INCT-PlantStress-Biotech team , held in April/2017, December/2018 and May/2019. Organization of the 7th Brazilian Congress of Biotechnology , organized by SBBIOTEC (Brazilian Society of Biotechnology), whose current president is Dr. Maria Fatima Grossi-de-Sá, held from 18 to 21 November 2018.

Learn more about the project coordinator, her resume and recent publications. Maria Fatima Grossi-de-Sa Coordinator PlantStress Biotech INCT Researcher Group Leader at Embrapa Genetic Resources and Biotechnology Lattes: http://lattes.cnpq.br/3058512809761818 E-mail: fatima.grossi@embrapa.br Phone number: +55 (61) 3448-4902 PROFILE Maria Fatima Grossi-de-Sa is a Researcher Group Leader at EMBRAPA Genetic Resources and Biotechnology and Professor at the Catholic University of Brasília, Brasília-DF, Brazil. She is also the Coordinator of the National Institute of Science and Technology – PlantStress Biotech INCT at EMBRAPA and Director President of the Brazilian Society of Biotechnology. She is a full member of the Brazilian Academy of Sciences and the World Academy of Science (TWAS). In 2018, she received the Commander medal of the National Order of Scientific Merit. She works in a wide range of research interests with an emphasis on plant biotechnology. Her main research interests are: plant-pest molecular interactions, focused on phytonematodes and insect-pests; development of GM crop plants for tolerance/resistance to abiotic and biotic stresses; understanding the molecular RNAi mechanism on insect pests, and; development of biopharmaceuticals. She is classified as scientific researcher 1A at the Brazilian National Research Council (CNPq), and has coordinated several research projects finnaced by EMBRAPA, FAP-DF, CAPES, and CNPq. Currently, her research group is funded by industries and the productive sector of agribusiness. EDUCATION B.Sc. In Biological Sciences, University of Brasilia, Brazil, 1979. M.Sc. In Molecular Biology, University of Brasilia, Brazil, 1982. Ph.D. University PARIS VII, Paris, France, 1987. CURRENT POSITION Researcher Leader on Plant Biotechnology at EMBRAPA Genetic Resources and Biotechnology - Brasilia- DF, Brazil (since 1989). Senior Associate Professor at Catholic University of Brasilia (UCB), Brasilia-DF, Brazil (since 2004). President of Brazilian Society of Biotechnology (since 2016). Director of the National Institute Science & Technology on Plant Stress Biotechnology (INCT PlantStress Biotech; since 2016). HONOURS/AWARDS Award of Excellence in recognition of Outstanding Research Performance by Embrapa (2009). Award Brazil SCOPUS Awards 2010 - Elsevier/CAPES (2010). Full elected member of Brazilian Academy of Sciences (Elected May 2011). Full elected member of the World Academy of Sciences (Elected Nov 2014). National Order of Scientific Merit - Commander Class, Ministry of Science and Technology - Presidency of the Republic (2018). RESEARCH INTERESTS The main research interest focuses on novel and innovative approaches to crop protection, plant-nematode and insect interactions, transgenic crops, biotic and abiotic stresses, plant genome editing, gene silencing, insect nanobiotechnology, biopesticides based on RNA interference approaches, and recombinant proteins. RELEVANT PAPERS (LAST 7 YEARS) 2023 ATELLA, A. L.; Grossi-de-Sa, M. F.; Alves-Ferreira, M. (2023). Cotton promoters for controlled gene expression. Electronic Journal of Biotechnology, v. 62, p. 10.1016/j.ejbt. https://doi.org/10.1016/j.ejbt.2022.12.002 BASSO, M. F.; Lourenço-Tessutti, I. T.; Moreira-Pinto, C. E.; Mendes, R. A. G.; Pereira, D. G.; Grandis, A.; Macedo, L. L. P.; Macedo, A. F.; Gomes, A. C. M. M.; Arraes, F. B. M.; Togawa, R. C.; do Carmo Costa, M. M.; Marcelino-Guimaraes, F. C.; Silva, M. C. M.; Floh, E. I. S.; Buckeridge, M. S., de Almeida Engler, J., Grossi-de-Sa, M. F. (2023). Overexpression of the GmEXPA1 gene reduces plant susceptibility to Meloidogyne incognita . Plant Cell Report , v. 42, n. 1, p. 137-152. https://doi.org/10.1007/s00299-022-02941-3 TRENZ, T. S.; Turchetto-Zolet, A. C.; Margis, R.; Margis-Pinheiro, M.; Maraschin, F. S. (2023) . Functional analysis of alternative castor bean DGAT enzymes. Genetics and Molecular Biology (Online Version), v. 46, p. 1-12. https://doi.org/10.1590/1678-4685-GMB-2022-0097 2022 ARRAES, F.B.M.; Vasquez, D.D.N.; Tahir, M.; Pinheiro, D.H.; Faheem, M.; Freitas-Alves, N.S.; Moreira-Pinto, C.E.; Moreira, V.J.V.; Paes-de-Melo, B.; Lisei-de-Sa, M.E.; Morgante, C.V.; Mota, A.P.Z.; Lourenço-Tessutti, I.T.; Togawa, R.C.; Grynberg, P.; Fragoso, R.R.; de Almeida-Engler, J.; Larsen, M.R.; Grossi-de-Sa, M.F. (2022). Integrated omic approaches reveal molecular mechanisms of tolerance during soybean and Meloidogyne incognita interactions. Plants , v. 11, n. 2744. https://doi.org/10.3390/plants11202744 BASSO, M.F.; Lourenço-Tessutti, I.T.; Moreira-Pinto, C.E.; Mendes, R.A.G.; Pereira, D.G.; Grandis, A. Macedo, L.L.P.; Macedo, A.F.; Gomes, A.C.M.M.; Arraes, F.B.M.; Togawa, R.C.; Costa, M.M.C.C.; Marcelino-Guimaraes, F.C.; Silva, M.C.M.; Floh, E.I.S.; Buckeridge, M.S.; de Almeida Engler, J.; Grossi-de-Sa, M.F. (2022). Overexpression of the GmEXPA1 gene reduces plant susceptibility to Meloidogyne incognita . Plant Cell Reports . https://doi.org/10.1007/s00299-022-02941-3 FRAGOSO, R.R.; Arraes, F.B.M.; Lourenço-Tessutti, I.T.; Miranda, V.J.; Basso, M.F.; Ferreira, A.V.J.; Viana, A.A.B.; Lins, C.B.J.; Lins, P.C.; Moura, S.M.; Batista, J.A.N.; Silva, M.C.M.; Engler, G.; Morgante, C.V.; Lisei-de-Sa, M.E.; Vasques, R.M.; de Almeida-Engler J.; Grossi-de-Sa, M.F. (2022). Functional characterization of the pUceS8.3 promoter and its potential use for ectopic gene overexpression. Planta , v. 256, n. 4, p. 1-18. https://doi.org/10.1007/s00425-022-03980-6 HABIBI, P.; Shi, Y.; Fatima Grossi-de-Sa, M. ; Khan I. (2022). Plants as sources of natural and recombinant antimalaria agents. Molecular Biotechnology . https://doi.org/10.1007/s12033-022-00499-9 KARALIJA, E.; Vergata, C.; Basso, M.F.; Negussu, M.; Zaccai, M.; Grossi-de-Sa, M.F. ; Martinelli, F. Chickpeas’. (2022). Tolerance of Drought and Heat: Current Knowledge and Next Steps. Agronomy , v. 12, n. 2248. https://doi.org/10.3390/agronomy12102248 MENDES, R.A.G.; Basso, M.F.; Amora, D.X.; Silva, A.P.; Paes-de-Melo, B.; Togawa, R.C.; Albuquerque, E.V.S.; Lisei-de-Sa, M.E.; Macedo, L.L.P.; Lourenço-Tessutti, I.T.; Grossi-de-Sa, M.F. (2022). In planta RNAi approach targeting three M. incognita effector genes disturbed the process of infection and reduced plant susceptibility. Experimental Parasitolog y, v. 238, p. 108246, https://doi.org/10.1016/j.exppara.2022.108246 MOREIRA, V.J.V.; Lourenço-Tessutti, I.T.; Basso, M.F.; Lisei-de-Sa, M.E.; Morgante, C.V.; Paes-de-Melo, B.; Arraes, F.B.M.; Martins-de-Sa, D.; Silva, M.C.M.; de Almeida Engler, J.; Grossi-de-Sa, M.F. (2022). Minc03328 effector gene downregulation severely affects Meloidogyne incognita parasitism in transgenic Arabidopsis thaliana . Planta , v. 255, p. 44-59. https://doi.org/10.1007/s00425-022-03823-4 MOURA, S.M.; Babilonia, K.; de Macedo, L.L.P.; Grossi-de-Sá, M.F. ; Shan, L.; He, P.; Alves-Ferreira, M. (2022). The oral secretion from Cotton Boll Weevil (Anthonomus grandis) induces defense responses in cotton (Gossypium spp) and Arabidopsis thaliana . Current Plant Biology , p. 100250. https://doi.org/10.1016/j.cpb.2022.100250 MOURA, S.M.; Freitas, E.O.; Ribeiro, T.P.; Paes-de-Melo, B.; Arraes, B.M.F.; Macedo, L.L.P.; Paixão, J.F.R.; Lourenço-Tessutti, I.T.; Artico, S.; Valença, D.C.; Silva, M.C.M.; Oliveira, A.C.; Alves-Ferreira, M.; Grossi-de-Sa, M.F. (2022). Discovery and functional characterization of novel cotton promoters with potential application to pest control. Plant Cell Reports , v. 41, p. 1589–1601. https://doi.org/10.1007/s00299-022-02880-z REIS, M.A.; Noriega, D.D.; dos Santos Alves, G.; Coelho, R.R.; Grossi-de-Sa, M.F. ; Antonino, J.D. (2022). Why is oral-induced RNAi inefficient in Diatraea saccharalis ? A possible role for DsREase and other nucleases. Pesticide Biochemistry and Physiology, v. 186, p. 105166. https://doi.org/10.1016/j.pestbp.2022.105166 RIBEIRO, T.P.; Vasquez, D.D.N.; Macedo, L.L.P.; Lourenço-Tessutti, I.T.; Valença, D.C.; Oliveira-Neto, O.B.; Paes-de-Melo, B.; Rodrigues-Silva, P.L.; Firmino, A.A.P.; Basso, M.F.; Lins, C.B.J.; Neves, M.R.; Moura, S.M.; Tripode, B.M.D.; Miranda, J.E.; Silva, M.C.M.; Grossi-de-Sa, M.F. (2022). Stabilized Double-Stranded RNA Strategy Improves Cotton Resistance to CBW (Anthonomus grandis ). International Journal of Molecular Sciences , v. 23, n. 13713. https://doi.org/10.3390/ijms232213713 RIBEIRO, D.G.; Mota, A.P.Z.; Santos, I.R.; Arraes, F.B.M.; Grynberg, P.; Fontes, W.; Castro, M.S.; Sousa, M.V.; Lisei-de-Sá, M.E.; Grossi-de-Sa, M.F. ; Franco, O.L.; Mehta, A. (2022). NBS-LRR-WRKY genes and protease inhibitors (PIs) seem essential for cowpea resistance to root-knot nematode. Journal of Proteomics , p. 104575. https://doi.org/10.1016/j.jprot.2022.104575 dos SANTOS, C., Carmo, L.S.T., Távora, F.T.P.K.; Lima, R.F.C.; Mendes, P.N.; Labuto, L.B.D.; de Sá, M.E.L.; Grossi-de-Sa, M.F.; Mehta, A. (2022). Overexpression of cotton genes GhDIR4 and GhPRXIIB in Arabidopsis thaliana improves plant resistance to root-knot nematode (Meloidogyne incognita ) infection. 3 Biotech , v. 12, p. 211. https://doi.org/10.1007/s13205-022-03282-4 TÁVORA, F.T.P.K.; Diniz, F.A.S.; Rêgo-Machado M.C.; Freitas, N.C.; Arraes, F.B.M.; Andrade, C.E.; Furtado, L.L.; Osiro, K.O.; Lima de Sousa, N.; Cardoso, T.B.; Henning, L.M.M.; Molinari, P.A.O.; Feingold, S.E.; Hunter, W.B.; Grossi de Sá, M.F. ; Kobayashi, A.K.; Nepomuceno, A.L.; Santiago, T.R.; Molinari, H.B.C. (2022). CRISPR/Cas- and topical RNAi-based technologies for crop management and improvement: reviewing the risk assessment and challenges towards a more sustainable agriculture. Frontiers in Bioengineering and Biotechnology , v. 10, p. 913728. https://doi.org/10.3389/fbioe.2022.913728 2021 ARAUJO SOUSA, B.; Nascimento Silva, O.; Farias Porto, W.; Lima Rocha, T.; Paulino Silva, L.; Ferreira Leal, A.P.; Buccini, D.F.; Oluwagbamigbe Fajemiroye, J.; de Araujo Caldas, R.; Franco, O.L.; Grossi-De-Sá, M.F. ; de La Fuente Nunez, C.; Moreno, S.E. (2021). Identification of the active principle conferring anti inflammatory and antinociceptive properties in bamboo plant. Molecules , v. 26, p. 3054. https://doi.org/10.3390/molecules26103054 ARRAES, F.B.M.; Martins-de-Sa, D.; Noriega Vasquez, D.D.; Melo, B.P.; Faheem, M.; de Macedo, L.L.P.; Morgante, C.V.; Barbosa, J.A.R.G.; Togawa, R.O.; Moreira, V.J.P.; Danchin, E.G.J.; Grossi-de-Sa, M.F. (2021). Dissecting protein domain variability in the core RNA interference machinery of five insect orders. RNA Biology , v. 18, p. 1653-1681. https://doi.org/10.1080/15476286.2020.1861816 BASSO, M.F.; Costa, J.A.; Ribeiro, T.P.; Arraes, F.B.M.; Lourenço-Tessutti, I.T.; Macedo, A.F.; Neves, M.R.; Nardeli, S.M.; Arge, L.W.; Perez, C.E.A.; Silva, P.L.R; De Macedo, L.L.P.; Lisei-de-Sa, M.E.; Amorim, R.M.A.; Pinto, E.R.C.; Silva, M.C.M.; Morgante, C.V.; Floh, E.I.S.; Alves-Ferreira, M.; Grossi-de-Sa, M.F. (2021). Overexpression of the CaHB12 transcription factor in cotton ( Gossypium hirsutum ) improves drought tolerance. Plant Physiology and Biochemistry , v. 165, p. 80-93. https://doi.org/10.1016/j.plaphy.2021.05.009 CABRAL, D.; Forero Ballesteros, H.; de Melo, B.P.; Lourenço-Tessutti, I.T.; Smões de Siqueira, K.M.; Obicci, L.; Grossi-de-Sa, M.F. ; Hemerly, A.S.; de Almeida Engler, J. (2021). The armadillo BTB protein ABAP1 is a crucial player in DNA replication and transcription of nematode-induced galls. Frontiers in Plant Science , v. 12, p. 636663. https://doi.org/10.3389/fpls.2021.636663 LISEI-DE-SÁ, M.E.; Rodrigues-Silva, P.L.; Morgante, C.V.; de Melo, B.P.; Lourenço-Tessutti, I.T.; Arraes, F.B.M.; Sousa, J.P.A.; Galbieri, R.; Amorim, R.M.S.; de Lins, C.B.J.; Macedo, L.L.P.; Moreira, V.J.; Ferreira, G.F.; Ribeiro, T.P.; Fragoso, R.R.; Silva, M.C.M.; de Almeida-Engler, J.; Grossi-de-Sa, M.F. (2021). Pyramiding dsRNAs increases phytonematode tolerance in cotton plants. Planta , v. 254, p. 121. https://doi.org/10.1007/s00425-021-03776-0 MENDES, R.A.G.; Basso, M.F.; Paes-de-Melo, B.; Ribeiro, T.P.; Lima, R.N.; Araujo, J.F.; Grossi-de-Sa, M.; Mattos, V.S.; Togawa, R.C.; Albuquerque, E.V.S.; Lisei-de-Sa, M.E.; Silva, M.C.M.; Macedo, L.L.P.; Fragoso, R.R.; Fernandez, D.; Vignols, F.; Grossi-de-Sa, M.F. (2021). The Mi-EFF1/Minc17998 effector interacts with the soybean GmHub6 protein to promote host plant parasitism by Meloidogyne incognita . Physiological and Molecular Plant Pathology , v. 114, p. 101630. https://doi.org/10.1016/j.pmpp.2021.101630 MENDES, R.A.G.; Basso, M.F.; Fernandes de Araújo, J.; Paes De Melo, B.; Lima, R.N.; Ribeiro, T.P.; da Silva Mattos, V.; Saliba Albuquerque, E.V.; Grossi-De-Sa, M.; Dessaune Tameirao, S.N.; da Rocha Fragoso, R.; Mattar da Silva, M.C.; Vignols, F.; Fernandez, D.; Grossi-De-Sa, M.F. (2021). Minc00344 and Mj-NULG1a effectors interact with GmHub10 protein to promote the soybean parasitism by Meloidogyne incognita and M. javanica. Experimental Parasitology , v. 229, p. 108153. https://doi.org/10.1016/j.exppara.2021.108153 MOREIRA-PINTO, C.E.; Ramos Coelho, R.; Borges Leite, A.G.; Amaral Silveira, D.; Aguiar Souza, D.; Biaggioni Lopes, R.; Macedo, L.L.P.; Mattar Silva, M.C.; Ribeiro, T.P.; Morgante, C.V.; Antonino, J.D.; Grossi-de-Sa, M.F. (2021). Increasing susceptibility to through-induced knockdown: a perspective to combine biocontrol and biotechnology. Pest Management Science , v. 77, p. ps.6430. https://doi.org/10.1002/ps.6430 MOREIRA-PINTO, C.E.; Coelho, R.R.; Leite, A.G.B.; Silveira, D.A.; Souza, D.A.; Lopes, R.B.; Macedo, L.L.P.; Silva, M.C.M.; Ribeiro, T.P.; Antonino, J.D.; Grossi-de-Sa, M.F. (2021). Increasing Anthonomus grandis susceptibility to Metarhizium anisopliae through RNAi-induced AgraRelish knockdown: a perspective to combine biocontrol and biotechnology. Pest Management Science , v. 77, p. 4054-4063. https://doi.org/10.1002/ps.6430 MOTA, A.P.Z.; Brasileiro, A.C.M.; Vidigal, B.; Oliveira, T.N.; da Cunha Quintana Martins, A.; Saraiva, M.A.P.; de Araújo, A.C.G.; Togawa, R.C.; Grossi-de-Sá, M.F. ; Guimaraes, P.M. (2021). Defining the combined stress response in wild Arachis . Scientific Reports , v. 11, p. 11097. https://doi.org/10.1038/s41598-021-90607-7 PAES DE MELO, B.; Lourenço-Tessutti, I.T.; Fraga, O.T.; Pinheiro, L.B.; de Jesus Lins, C.B.; Morgante, C.V.; Engler, J.A.; Reis, P.A.B.; Grossi-De-Sá, M.F. ; Fontes, E.P.B. (2021). Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses. Scientific Reports , v. 11, p. 11178. https://doi.org/10.1038/s41598-021-90767-6 PAES DE MELO, B.; Moura, S.M.; Morgante, C.V.; Pinheiro, D.H.; Alves, N.S.F.; Rodrigues-Silva, P.L.; Lourenço-Tessutti, I.T.; Andrade, R.V.; Fragoso, R.R.; Grossi-de-Sa, M.F. (2021). Regulated promoters applied to plant engineering: an insight over promising soybean promoters under biotic stress and their cis-elements. Biotechnology Research and Innovation , v. 5, p. e2021005. http://dx.doi.org/10.4322/biori.202105 RIBEIRO, T.P.; Lourenço-Tessutti, I.T.; De Melo, B.P.; Morgante, C.V.; Filho, A.S.; Lins, C.B.J.; Ferreira, G.F.; Mello, G.N.; Macedo, L.L.P.; Lucena, W.A.; Silva, M.C.M.; Oliveira-Neto, O.B.; Grossi-de-Sa, M.F. (2021). Improved cotton transformation protocol mediated by Agrobacterium and biolistic combined-methods. Planta , v. 254, p. 20. https://doi.org/10.1007/s00425-021-03666-5 RODRIGUES-SILVA, P.L.; Amorim, G.C.; Andrade, I.E.P.C.; Cunha, V.A.; Figueiredo, L.H.M.; Grossi-de-Sa, M.F. (2021). Monitoramento tecnológico da planta cagaita (Eugenia dysenterica ) e aplicações biotecnológicas potenciais. Cadernos de Prospecção , v. 14, p. 1248-1264. https://doi.org/10.9771/cp.v14i4.38459 RODRIGUES-SILVA, P.L.; Fernandes, P.B.B.; Rodrigues, M.T.; Figueiredo, L. H. M.; Grossi-de-Sa, M.F. (2021). Tendências quanto ao conhecimento e às aplicações biotecnológicas do Psidium guineense evidenciadas pelo monitoramento tecnológico. Cadernos de Ciência & Tecnologia , v. 38, p. e26704. https://doi.org/10.35977/0104-1096.cct2021.v38.26704 2020 BASSO, M.F.; Arraes, F.B.M.; Grossi-de-Sa, M.; Vaz-Moreira, V.J.; Alves-Ferreira, M.; Grossi-de-Sa, M.F. (2020). Insights into genetic and molecular elements for transgenic crop development. Frontiers in Plant Science , v. 11, p. 509. https://doi.org/10.3389/fpls.2020.00509 BASSO, M.F.; Lourenço-Tessutti, I.T.; Busanello, C.; Pinto, C.E.M.; Oliveira-Freitas, E.; Ribeiro, T.P.; Almeida-Engler, J.; Oliveira, A.C.; Morgante, C.V.; Alves-Ferreira, M.; Grossi-de-Sa, M.F. (2020). Insights obtained using different modules of the cotton uceA1.7 promoter. Planta , v. 251, p. 56. https://doi.org/10.1007/s00425-020-03348-8 BASSO, M.F.; Lourenço-Tessutti, I.T.; Mendes, R.A.G.; Pinto, C.E.M.; Bournaud, C.; Gillet, F.X.; Togawa, R.C.; Macedo, L.L.P.; Almeida-Engler, J.; Grossi-de-Sa, M.F. (2020). MiDaf16-like and MiSkn1-like gene families are reliable targets to develop biotechnological tools for the control and management of Meloidogyne incognita. Scientific Reports , v. 10, p. 6991. https://doi.org/10.1038/s41598-020-63968-8 BEVITORI, R.; Sircar, S.; Mello, R.N.; Togawa, R.C.; Cortes, M.V.C.B.; Oliveira, T.S.; Grossi-de-Sa, M.F. ; Parekh, N. (2020). Identification of co-expression gene networks controlling rice blast disease during an incompatible reaction. Genetics Molecular Research , v. 19, p. gmr18579. https://doi.org/10.4238/gmr18579 CABRAL, D.N.; Banora, M.Y.; Antonino, J.D.; Rodiuc, N.; Vieira, P.; Coelho, R.R.; Chevalier, C.; Eekhout, T.; Engler G.; De-Veylder, L.; Grossi-de-Sa, M.F. ; Almeida-Engler, J. (2020). The plant WEE1 kinase is involved in checkpoint control activation in nematode-induced galls. New Phytologist , v. 225(1), p. 430-447. https://doi.org/10.1111/nph.16185 CAMPOS, M.L.; Prado, G.S.; Santos, V.O.; Nascimento, L.C.; Dohms, S.M.; Cunha, N.B.; Ramada, M.H.S.; Grossi-de-Sa, M.F. ; Dias, S.C. (2020). Mosses: versatile plants for biotechnological applications. Biotechnology Advances , v. 6, p. 107533. https://doi.org/10.1016/j.biotechadv.2020.107533 FIRMINO, A.A.P.; Pinheiro, D.H.; Pinto, C.E.M.; Antonino, J.D.; Macedo, L.L.P.; Martins-de-Sa, D.; Arraes, F.B.M.; Coelho, R.R.; Fonseca, F.C.A.; Silva, M.C.M.; Almeida-Engler, J.; Silva, M.S.; Lourenço-Tessutti, I.T.; Terra, W.R.; Grossi-de-Sa, M.F. (2020). RNAi-mediated suppression of Laccase2 impairs cuticle tanning and molting in the cotton boll weevil (Anthonomus grandis ). Frontiers in Physiology , v. 11, p. 591569. https://doi.org/10.3389/fphys.2020.591569 GRYNBERG, P.; Togawa, R.C.; Freitas, L.D.; Antonino, J.D.; Rancurel, C.; Costa, M.M.C.; Grossi-de-Sa, M.F. ; Miller, R.N.G.; Brasileiro, A.C.M.; Guimaraes, P.M.; Danchin, E.G.J. (2020). Comparative genomics reveals novel target genes towards specific control of plant-parasitic nematodes. Genes , v. 11, p. 1347. https://doi.org/10.3390/genes11111347 IBARRA, L.N.; Alves, A.E.O.A.; Antonino, J.D.; Prado, G.S.; Pinto, C.E.M.; Soccol, C.R.; Vasconcelos, E.A.R.; Grossi-de-Sa, M.F. (2020). Enzymatic activity of a recombinant β-1,4-endoglucanase from the cotton boll weevil (Anthonomus grandis ) aiming second generation ethanol production. Scientific Reports , v. 10(1), p. 5367. https://doi.org/10.1038/s41598-019-56070-1 MOTA, A.P.Z.; Fernandez, D.; Arraes, F.B.M.; Petitot, A.S.; Paes-Melo, B.; Lisei-de-Sa, M.E.; Guimaraes, P.M.; Brasileiro, A.C.M.; Albuquerque, E.V.S.; Danchin, E.G.J.; Grossi-de-Sa, M.F. (2020). Evolutionarily conserved plant genes responsive to root-knot nematodes identified by comparative genomics. Molecular Genetics and Genomics , v. 295, p. 1063-1078. https://doi.org/10.1007/s00438-020-01677-7 MOURA, S.M.; Rossi, M.L.; Artico, S.; Grossi-de-Sa, M.F. ; Martinelli, A.P.; Alves-Ferreira, M. (2020). Characterization of floral morphoanatomy and identification of marker genes preferentially expressed during specific stages of cotton flower development. Planta , v. 252(4), p. 71. https://doi.org/10.1007/s00425-020-03477-0 NORIEGA-VASQUEZ, D.D.; Arraes, F.B.M.; Antonino, J.D.; Macedo, L.L.P.; Fonseca, F.C.A.; Togawa, R.C.; Grynberg, P.; Silva, M.C.M.; Negrisoli, A.S.; Grossi-de-Sa, M.F. (2020). Transcriptome analysis and knockdown of the juvenile hormone esterase gene reveal abnormal feeding behavior in the sugarcane giant borer. Frontiers in Physiology , v. 11, p. 588450. https://doi.org/10.3389/fphys.2020.588450 NORIEGA-VASQUEZ, D.D.; Arraes, F.B.M.; Antonino, J.D.; Macedo, L.L.P.; Fonseca, F.C.A.; Togawa, R.C.; Grynberg, P.; Silva, M.C.M.; Negrisoli, A.S.; Morgante, C.V.; Grossi-de-Sa, M.F. (2020). Comparative gut transcriptome analysis of Diatraea saccharalis in response to the dietary source. PLoS One , v. 15(8), p. e0235575. https://doi.org/10.1371/journal.pone.0235575 PAES-MELO, B.; Lourenço-Tessutti, I.T.; Morgante, C.V.; Santos, N.C.; Pinheiro, L.B.; Jesus-Lins, C.B.; Silva, M.C.M.; Macedo, L.L.P.; Fontes, E.P.B.; Grossi-de-Sa, M.F. (2020). Soybean embryonic axis transformation: combining biolistic and Agrobacterium -mediated protocols to overcome typical complications of in vitro plant regeneration. Frontiers in Plant Science , v. 11, p. 1228. https://doi.org/10.3389/fpls.2020.01228 PAES-MELO, B.; Lourenço-Tessutti, I.T.; Paixao, J.F.R.; Noriega-Vasquez, D.D.; Silva, M.C.M.; Almeida-Engler, J.; Fontes, E.P.B.; Grossi-de-Sa, M.F. (2020). Transcriptional modulation of AREB-1 by CRISPRa improves plant physiological performance under severe water deficit. Scientific Reports , v. 10(1), p. 16231. https://doi.org/10.1038/s41598-020-72464-y RIBEIRO, T.P.; Basso, M.F.; Carvalho, M.H.; Macedo, L.L.P.; Silva, D.M.L.S.; Lourenço-Tessutti, I.T.; Oliveira-Neto, O.B.; Romano, E.; Lucena, W.A.; Silva, M.C.M.; Tripode, B.M.D.; Abreu-Jardin, T.P.F.; Miranda, J.E.; Alves-Ferreira, M.; Morgante, C.V.; Grossi-de-Sa, M.F. (2020). Stability and tissue-specific Cry10Aa overexpression improves cotton resistance to the cotton boll weevil. 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Biotechnology Research & Innovation , v. 3(1), p. 69-79. https://doi.org/10.1016/j.biori.2019.04.003 FREITAS, E.O.; Paes-Melo, B.; Lourenço-Tessutti, I.T.; Arraes, F.B.M.; Amorim, R.M.S.; Lisei-de-Sa, M.E.; Costa, J.A.; Leite, A.G.B.; Faheem, M.; Ferreira, M.A.; Morgante, C.V.; Fontes, E.P.B.; Grossi-de-Sa, M.F. (2019). Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application. BMC Biotechnology , v. 19(1), p. 79. https://doi.org/10.1186/s12896-019-0561-3 GROSSI-DE-SA, M.; Petitot, A.S.; Xavier, D.A.; Lisei-de-Sa, M.E.; Mezzalira, I.; Beneventi, M.A.; Martins, N.F.; Baimey, H.K.; Albuquerque, E.V.S.; Grossi-de-Sa, M.F. ; Fernandez, D. (2019). Rice susceptibility to root-knot nematodes is enhanced by the Meloidogyne incognita MSP18 effector gene. Planta , v. 250, p. 1215-1227. https://doi.org/10.1007/s00425-019-03205-3 HABIBI, P.; Henry, D.; Soccol, C.R.; Grossi-de-Sa, M.F. (2019). The potential of plant systems to break the HIV-TB link. Plant Biotechnology Journal , v. 17(10), p. 1868-1891. https://doi.org/10.1111/pbi.13110 IBARRA, L.N.; Alves, A.N.O.A.; Antonino, J.D.; Prado, G.S.; Pinto, C.E.M.; Soccol, C.R.; Vasconcelos, E.A.R.; Grossi-de-Sa, M.F. (2019). Enzymatic activity of a recombinant β-1,4-endoglucanase from the Cotton Boll Weevil ( Anthonomus grandis ) aiming second generation ethanol production. Scientific Reports , v. 9, p. 19580. https://doi.org/10.1038/s41598-019-56070-1 MATTOS, V.S.; Mulet, K.; Cares, J.E.; Gomes, C.B.; Fernandez, D.; Grossi-de-Sa, M.F. ; Carneiro, R.M.D.G.; Castagnone-Sereno, P. (2019). Development of diagnostic SCAR markers for Meloidogyne graminicola, M. oryzae , and M. salasi associated with irrigated rice fields in Americas. 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Scientific Reports , v. 9(1), p. 12804. https://doi.org/10.1038/s41598-019-49178-x PAIXAO, J.F.R.; Gillet, F.X.; Ribeiro, T.P.; Bournaud, C.; Lourenço-Tessutti, I.T.; Noriega-Vasquez, D.D.; Paes-Melo, B.; Almeida-Engler, J.; Grossi-de-Sa, M.F. (2019). Improved drought stress tolerance in Arabidopsis by CRISPR/dCas9 fusion with a Histone AcetylTransferase. Scientific Reports , v. 9(1), p. 8080. https://doi.org/10.1038/s41598-019-44571-y PRADO, G.S.; Bamogo, P.K.A.; Abreu, J.A.C.; Gillet, F.X.; Santos, V.O.; Silva, M.C.M.; Brizard, J.P.; Bemquerer, M.P.; Bangratz, M.; Brugidou, C.; Sérémé, D.; Grossi-de-Sa, M.F. ; Lacombe, S. (2019). Nicotiana benthamiana is a suitable transient system for high-level expression of an active inhibitor of cotton boll weevil α-amylase. BMC Biotechnology , v. 19(1), p. 15. https://doi.org/10.1186/s12896-019-0507-9 SANTOS, CRISTIANE; Nogueira, F.C.S.; Domont, G.B.; Fontes, W.; Prado, G.S.; Habibi, P.; Santos, V.O.; Oliveira-Neto, O.B.; Grossi-De-Sá, M.F. ; Jorrín-Novo, J.V.; Franco, O.L.; Mehta, A. (2019). Proteomic analysis and functional validation of a Brassica oleracea Endochitinase involved in resistance to Xanthomonas campestris. Frontiers in Plant Science , v. 10, p. 414. https://doi.org/10.3389/fpls.2019.00414 2018 BOURNAUD, C.; Gillet, F.X.; Murad, A.M.; Bresso, E.; Albuquerque, E.V.S.; Grossi-de-Sa, M.F. (2018). Meloidogyne incognita PASSE-MURAILLE (MiPM ) gene encodes a cell-penetrating protein that interacts with the CSN5 subunit of the COP9 signalosome. Frontiers in Plant Science , v. 9, p. 904. https://doi.org/10.3389/fpls.2018.00904 HABIBI, P.; Soccol, C.R.; Okeefe, B.R.; Krumpe, L.R.H.; Wilson, J.; Macedo, L.L.P.; Faheem, M.; Santos, V.O.; Prado, G.Z.; Botelho, M.A.; Lacombe, S.; Grossi-de-Sa, M.F. (2018). Gene-silencing suppressors for high-level production of the HIV-1 entry inhibitor griffithsin in Nicotiana benthamiana. Process Biochemistry , v. 70, p. 45-54. https://doi.org/10.1016/j.procbio.2018.04.006 MATTOS, V. S.; Mulet, K.; Cares, J. E.; Gomes, C. B.; Fernandez, D.; Grossi-de-Sa, M. F.; Carneiro, R. M. D. G.; Castagnone-Sereno, P. (2018). Development of diagnostic SCAR markers for Meloidogyne graminicola, M. oryzae, and M. salasi associated with irrigated rice fields in Americas. Plant Disease, v. 103, p. 88-83. https://doi.org/10.1094/PDIS-12-17-2015-RE NARDELI, S.M.; Artico, S.; Aoyagi, G.M.; Moura, S.M.; Franca-Silva, T.; Grossi-de-Sa, M.F. ; Romanel, E.; Alves-Ferreira, M. (2018). Genome-wide analysis of the MADS-box gene family in polyploid cotton (Gossypium hirsutum ) and in its diploid parental species (Gossypium arboreum and Gossypium raimondii ). Plant Physiology and Biochemistry , v. 127, p. 169-184. https://doi.org/10.1016/j.plaphy.2018.03.019 SILVA, M.S.; Arraes, F.B.M.; Campos, M.A.; Grossi-de-Sa, M.; Fernandez, D.; Cândido, E.S.; Cardoso, M.H.; Franco, O.L.; Grossi-de-Sa, M.F. (2018). Review: potential biotechnological assets related to plant immunity modulation applicable in engineering disease-resistant crops. Plant Science , v. 270, p. 72-84. https://doi.org/10.1016/j.plantsci.2018.02.013 2017 ALBUQUERQUE, E.V.S.; Petitot, A.S.; Silva, J.P.; Grossi-de-Sa, M.F. ; Fernandez, D. (2017). Early responses of coffee immunity-related genes to root-knot nematode infection. Physiological and Molecular Plant Pathology , v. 100, p. 142-150. https://doi.org/10.1016/j.pmpp.2017.09.001 ALMEIDA-GARCIA, R.; Macedo, L.L.P.; Cabral, D.N.; Gillet, F.X.; Pinto, C.E.M.; Faheem, M.; Basso, A.M.M.; Silva, M.C.M.; Grossi-de-Sa, M.F. (2017). Nucleases as a barrier to gene silencing in the cotton boll weevil, Anthonomus grandis. PLoS One , v. 12(12), p. e0189600. https://doi.org/10.1371/journal.pone.0189600 ANTONINO, J.D.; Pierre, O.; Coelho, R.R.; Grossi-de-Sa, M.F. ; Engler, G.; Almeida-Engler, J. (2017). Application of nuclear volume measurements to comprehend the cell cycle in root-knot nematode-induced giant cells. Frontiers in Plant Science , v. 8, p. 961. https://doi.org/10.3389/fpls.2017.00961 GILLET, F.X.; Almeida-Garcia, R.; Macedo, L.L.P.; Albuquerque, E.V.S.; Silva, M.C.M.; Grossi-de-Sa, M.F. (2017). Investigating engineered ribonucleoprotein particles to improve oral RNAi delivery in crop insect pests. Frontiers in Physiology , v. 8, p. 1-14. https://doi.org/10.3389/fphys.2017.00256 GILLET, F.X.; Bournaud, C.; Antonino, J.D.; Grossi-de-Sa, M.F. (2017). Plant-parasitic nematodes: towards understanding molecular players in stress responses. Annals of Botany , v. 119, p. 260-775. https://doi.org/10.1093/aob/mcw260 HABIBI, P; Prado, G.S.; Pelegrini, P.B.; Hefferson, K.L.; Soccol, C.R.; Grossi-de-Sa, M.F. (2017). Optimization of inside and outside factors to improve recombinant protein yield in plant. Plant Cell Tissue and Organ Culture , v. 130, p. 449-467. https://doi.org/10.1007/s11240-017-1240-5 LISEI-DE-SA, M.E.; Arraes, F.B.M.; Brito, G.; Beneventi, M.A.; Lourenço-Tessutti, I.T.; Basso, A.M.M.; Amorim, R.M.S.; Silva, M.C.M.; Faheem, M., Oliveira, N.G.; Mizoi, J.; Yamaguchi-Shinozaki, K.; Grossi-de-Sa, M.F. (2017). AtDREB2A-CA influences root architecture and increases drought tolerance in transgenic cotton. Agricultural Sciences , v. 8(10), p. 1195-1225. https://doi.org/10.4236/as.2017.810087 MOURA, S.M.; Artico, S.; Lima, C.; Nardeli, S.M.; Berbel, A.; Oliveira-Neto, O.B.; Grossi-de-Sa, M.F. ; Ferrándiz, C.; Madueño, F.; Alves-Ferreira, M. (2017). Functional characterization of AGAMOUS-subfamily members from cotton during reproductive development and in response to plant hormones. Plant Reproduction, v. 30(1), p. 19-39. https://doi.org/10.1007/s00497-017-0297-y RAMOS, C.R.; Vieira, P.; Antonino, J.D.; Martin-Jimenez, C.; De-Veylder, L.; Cazareth, J.; Engler, G.; Grossi-de-Sa, M.F. ; Almeida-Engler, J. (2017). Exploiting cell cycle inhibitor genes of the KRP family to control root-knot nematode induced feeding sites in plants. Plant, Cell and Environment , v. 40, p. 1174-1188. https://doi.org/10.1111/pce.12912 RIBEIRO, T.P.; Arraes, F.B.M.; Lourenço-Tessutti, I.T.; Silva, M.S.; Lisei-de-Sa, M.E.; Lucena, W.A.; Macedo, L.L.P.; Lima, J.N.; Amorim, R.M.S.; Artico, S.; Alves-Ferreira, M.; Silva, M.C.M.; Grossi-de-Sa, M.F. (2017). Transgenic cotton expressing Cry10Aa toxin confers high resistance to the cotton boll weevil. Plant Biotechnology Journal , v. 15(8), p. 997-1009. https://doi.org/10.1111/pbi.12694 2016 OLIVEIRA, R.S.; Oliveira-Neto, O.B.; Moura, H.F.; Macedo, L.L.P.; Arraes, F.B.M.; Lucena, W.A.; Lourenço-Tessutti, I.T.; Barbosa, A.A.D.; Silva, M.C.M.; Grossi-de-Sa, M.F. (2016). Transgenic cotton plants expressing Cry1Ia12 toxin confer resistance to fall armyworm (Spodoptera frugiperda ) and cotton boll weevil (Anthonomus grandis ). Frontiers in Plant Science , v. 7, p. 165. https://doi.org/10.3389/fpls.2016.00165 LACERDA, A.F.; Pelegrini, P.B.; Oliveira, D.M.; Vasconcelos, E.A.; Grossi-de-Sa, M.F. (2016). Anti-parasitic peptides from arthropods and their application in drug therapy. Frontiers in Microbiology , v. 7, p. 91. https://doi.org/10.3389/fmicb.2016.00091 COELHO, R.R.; Antonino, J.D.; Firmino, A.A.P.; Macedo, L.L.P.; Fonseca, F.C.A.; Terra, W.R.; Engler, G.; Almeida-Engler, J.; Silva, M.C.M.; Grossi-de-Sa, M.F . (2016). Vitellogenin knockdown strongly affects cotton boll weevil egg viability but not the number of eggs laid by females. Meta Gene , v. 9, p. 173-80. https://doi.org/10.1016/j.mgene.2016.06.005 GUIMARAES-DIAS, F.; Neves-Borges, A.C.; Conforte, A.J.; Giovanella-Kampmann, L.; Ferreira, A.V.J.; Amorim, R.M.S.; Beneventi, M.A.; Lisei-de-Sa, M.E.; Mesquita, R.O.; Rodrigues, F.A.; Nepomuceno, A.L.; Romano, E.; Loureiro, M.E.; Grossi-de-Sa, M.F. ; Alves-Ferreira, M. (2016). Differential impact of acclimation and acute water deprivation in the expression of key transcription factors in soybean roots. Plant Molecular Biology Reporter , v. 34, p. 1167-1180. https://doi.org/10.1007/s11105-016-0993-z VILLETH, G.R.; Carmo, L.S.; Silva, L.P.; Santos, M.F.; Oliveira-Neto, O.B.; Grossi-de-Sa, M.F. ; Ribeiro, I.S.; Dessaune, S.N.; Fragoso, R.R.; Franco, O.L.; Mehta, A. (2016). Identification of proteins in susceptible and resistant Brassica oleracea responsive to Xanthomonas campestris pv. campestris infection. Journal Proteomics , v. 143, p. 278-285. https://doi.org/10.1016/j.jprot.2016.01.014 PATENTS HEMERLY, A.S.; Cavalcanti, P.F.; Gong, P.; Nelissen, H.; Inze, D.; Grossi-de-Sa, M.F. ; Basso, M.F.; Morgante, C.V.; Lisei-de-Sa, M.E. Method for promoting an increase in plant biomass, productivity, and drought resistance. US 2020/0347399 A1 . Deposit: May 4, 2020. Publication: November 5, 2020. GROSSI-DE-SA, M.F. ; Silva, M.C.M.; Fonseca, F.C.A.; Macedo, L.L.P.; Lourenco, I.T.; Albuquerque, E.V.S. Aparato e método de criação de larvas de insetos em laboratório. BR 1020130331120 B1 . Deposit: December 20, 2013. Patent granted: November 24, 2020. GROSSI-DE-SA, M.F. ; Oliveira, G.R.; Silva, M.C.M.; Rocha, T.L.; Magalhaes, M.T.Q. Molécula de ácido nucléico isolada, construção gênica, vetor, célula transgênica, método para obtenção de uma célula e de uma planta transgênica. PI 0906128-2 B1 . Deposit: July 24, 2009. Patent granted: March 17, 2020. GROSSI-DE-SA, M.F. ; Macedo, L.L.P.; Silva, M.C.M.; Almeida-Garcia, R.; Silva, L.P.; Vila, A. Pesticide. WO 2020/007450 A1 . Deposit: July 7, 2018, Publication: January 9, 2020. GROSSI-DE-SA, M.F. ; Macedo, L.L.P.; Pinto, C.E.M.; Leite, A.G.B.; Silva, M.C.M.; Lourenço-Tessutti, I.T.; Morgante, C.V. Método para produzir planta resistente a inseto praga e moléculas de ácido nucleicos utilizado para obtenção de tal planta através de dsRNAs relacionados a ecdise. PI 102020004312-9 . Deposit: March 3, 2020. ROCHA, T.L.; Evaristo, R.G.S.; Grossi-de-Sa, M.F. ; Silva, M.C.M.; Polez, V.L.P.; Roessner, U.; Bacic, T. Nematotoxic composition of synergistic effect, use of a nematotoxic composition of synergistic effect. US 9750247 B2, CN 105007725 B . Deposit: December 26, 2013 (US and CH). Patent granted: September 5, 2017 (US) and April 4, 2019 (CH). MACEDO, L.L.P.; Grossi-de-Sa, M.F. ; Silva, M.C.M.; Almeida-Garcia, R.; Mendes, R.A.G.; Albuquerque, E.V.S. Increasing the efficiency of supression of gene expression by means of the use of RNA molecules with a stabilized structure. BR 10201700690-4 A2, WO 2018/184083 A1. Deposit: April 4, 2017 (BR) and March 29, 2018 (US). Publication: October 30, 2018 (BR) and November 11, 2018 (US). GROSSI-DE-SA, M.F. ; Silva, M.C.M.; Del-Sarto, R.P.; Rocha, T.L. Mutantes de inibidores de alfa amilases isolados de Phaseolus vulgaris com propriedades de controlar insetos-praga, composições contendo tais mutantes e métodos de obtenção dos mesmos e de linhagens transgênicas. PI 1102841-6 . Deposit: June 8, 2011. Patent granted: November 26, 2018. GROSSI-DE-SA, M.F. ; Coelho, R.R.; Firmino, A.A.P.; Macedo, L.L.P; Silva, M.C.M.; Lourenço-Tessutti, I.T. Método e composições para controle de insetos-praga em plantas por meio do silenciamento de genes da família da quintina sintase e da vitelogenina bem como alternativamente pela expressão do gene de uma toxina Cry. BR 102013032649-6 A2 . Deposit: December 18, 2013. Publication: February 10, 2016. SOUZA, D.S.L.; Silva, M.C.M.; Grossi-de-Sa, M.F. ; Evaristo, R.G.S.; Rocha, T.L.; Bacic, T.; Roessner, U.; Polez, V.L.P. Composição nematotóxica de efeito sinérgico, uso de composição nematotóxica de efeito sinérgico. BR 10201203314-6 A2 . Deposit: December 26, 2012. Patent granted: August 26, 2014. MESSENBERG, P.G.; Grossi-de-Sa, M.F. Métodos para detecção e identificação de Curtobacterium flaccumfaciens pv flaccumfaciens em uma amostra biológica e método de certificação de sementes. PI 9806477-0 . Deposit: June 23, 1998. Patent granted: April 29, 2014. GROSSI-DE-SA, M.F. ; Silva, M.C.M.; Macedo, L.L.P.; Firmino, A.A.P.; Coelho, R.R.; Lourenço-Tessuti, I.T. Método e composições para controle genético de insetos-praga em plantas de algodão através do silenciamento de genes de quitina sintases. BR 102012033539-5 A2 , US 10182571 B2 and WO 2014/100879 A2 . Deposit: December 28, 2012 (BR), December 27, 2013 (US) and June 27, 2013 (WO). Publication: August 18, 2015 (BR), January 22, 2019 (US) and January 3, 2014 (WO). GROSSI-DE-SA, M.F. ; Silva, M.C.M.; Gomes-Jr, J.E.; Lourenço-Tessuti, I.T.; Macedo, L.P.P.; Lucena, W.A.; Fonseca, F.C.A. Moléculas variantes sintéticas de toxinas Cry1Ia12 com propriedades de controlar insetos-praga, composições contendo tais mutantes e método de utilização dos mesmos. WO 2014-100880 A3 . Deposit: 02/12/2012. Publication: July 3, 2014. GROSSI-DE-SA, M.F. ; Romano, E.; Fragoso, R.R.; Silva, M.C.M.; Ferreira, A.V.J.; (2013). Cassete de expressão para indução de resistência a múltiplas espécies de nematóides em plantas, métodos e plantas que o utilizam. WO 2014/197951 A2 . Deposit: June 11, 2013. Publication: December 18, 2014. GROSSI-DE-SA, M.F. ; Firmino, A.A.P.; Silva, M.C.M.; Martins-de-Sa, D.; Coelho, R.R.; Macedo, L.L.P.; Lourenço-Tessuti, I.T. Método e composições para controle genético de insetos-praga em plantas de algodão através do silenciamento de genes de lacases. WO 2014/100878 A3. Deposit: December 27, 2013. Publication: July 3, 2014. ALVES-FERREIRA, M.; Grossi-de-Sa, M.F. ; Lambret, J.; Nardeli, S.M.; Artico, S. Compositions and method for modifying gene expression using a promoter of genes specific to plant flowers and fruit. WO 2014/000075 A2 . Deposit: June 27, 2012. Publication: January 3, 2014. GROSSI-DE-SA, M.F. ; Guimaraes, L.M.; Batista, J.A.N.; Viana, A.A.B.; Fragoso, R.R.; Rocha, T.L. Composições e métodos para modificar a expressão de genes usando o promotor do gene da proteína de conjugação à ubiquitina de plantas de algodoeiro. PI 0701230-6 , US 2013/0152226 A1 . Deposit: February 5, 2007 (BR) and February 19, 2013 (US). 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Find out more about the project, the specific objectives and the institutions involved. Acerca de About the Project Biological systems research focusing on elucidating plant stress mechanisms and signaling pathways is important for developing resistant cultivars in a climate change scenario. Pathogen attacks and abiotic stresses such as water deficit are essential during plant development, and the identification of genes and molecules, which regulate resistance responses, are critical to develop crop cultivars capable of doing in the face of these stresses. In association with abiotic stresses, plants must also defend themselves against pest’s attacks, including fungi, bacteria, insect pests, and nematodes. Insect-pests comprise the most serious factor affecting productivity losses in the Brazilian agribusiness. Losses in soybean, cotton, and corn caused by the caterpillars Helicoverpa armigera , Spodoptera frugiperda , and phytonematodes constitute the most critical phytosanitary problem in terms of competitiveness for the commodities production and exportation. Faced with scenario cases involving plant resistance breakdown, environmental association of biotic and abiotic stresses, fewer characterized and introgressed genes, and higher complexity of plant responses have been reported. All these reports demonstrate the importance of the research towards the identification of genes/molecules involved in pest resistance and water deficit responses. In this context, the National Institute of Science and Technology, PlantStress Biotech INCT, integrates various Brazilian research groups and international partners, experts in plant physiology, transcriptomic, epigenetic, proteomic, bioinformatic and functional genomics analyses. The integrative research group represents a multidisciplinary and multi-institutional network with national and international excellence to generate innovative biotechnologies applied to corn, soybean and cotton focused on the tolerance to deficit hydric and pest control (Meloidogyne spp, H. armigera and S. frugiperda ). The project includes bioprospection, isolation, characterization, and functional validation of genes/molecules involved in plant pest resistance and drought tolerance. The PlantStress Biotech INCT action comprises: Prospection for genes and molecules involved in resistance/tolerance to specific and combined stresses in native plants and resistant/tolerant contrasting genotypes, through the elucidation of the stress mechanisms and signaling pathways; Prospection for genes and molecules involved in the defense and parasitism of target pests; Search for promoters responsive to pests and water deficit, and small RNAs involved in the regulation of target genes; Use different strategies (gene/molecules overexpression or gene silencing) for biological functional validation in model systems; Developing of new nanoencapsulated insecticides/nematicides from validated molecules; Developing of biotechnological products (concept proof) in GM crop plants (cotton, soybean, and maize); Evaluation of developed GM crop plants in simulated field experiments. The biotech assets generated will be applied to the development of biotechnology products, including nanobioinsecticides and genetically modified crops (soybean, maize, and cotton) against biotic and abiotic stresses. In addition to the biotech assets obtained and characterized in this project, an understanding of molecular and physiological factors related to drought tolerance and their interaction with pests and other environmental stresses will contribute to prevent, unravel and adapt to climatic changes. Alongside its scientific agenda, the PlantStress Biotech INCT group, formed by researchers from five Embrapa units and five Federal Universities (UnB, UFRJ, UFRGS, UFC, and UFPel), and collaborators from the public and private sectors, concentrate their efforts on training students and professionals in different and update approaches involving biotechnology, genomics, and bioinformatics. Actions will also strengthen joint action and the internationalization of undergraduate and graduate training engaged in PlantStress Biotech INCT research, and to increase the visibility and international insertion of its teams. The PlantStress Biotech INCT network focuses not only on the generation of biotech assets applied to drought and pests in soybean, cotton, and maize but also on other important agronomic traits (seed and fruit quality, an increase of biomass and nutritional value, among other) and in other crops important for Brazilian agribusiness.

Learn more about AL03, its main goals within the project and meet the laboratory's work team. AL 03 - Molecular Genetics Laboratory Activities - PlantStress Biotech INCT Integration of the transcriptome and sequencing of the model plant (Setaria) submitted to drought and high carbon dioxide content. Validate the expression of key genes in the metabolic pathways of plants response to combined stresses (biotic-biotic; biotic-biotic; abiotic-biotic) by qRT-PCR. Identify in silico promoters responsive to biotic and abiotic stress (drought and phytonematodes), with activity in different organs (root and leaf). Set up a vector bank with promoters directing the expression of reporter genes (GUS/GFP) in monocots and dicots. Validate promoters by transient transformation by biolistics or Agrobacterium rhyzogenes in target plants for in vivo testing of promoters (soybean, cotton and corn). Analyze the sequencing data to check the methylation status of gene promoters in the metabolic pathways of interest. Validate the function of plant genes potentially involved in drought tolerance mechanisms in Arabidopsis , rice or sepia plants through strategies of overexpression or silencing. Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Márcio Alves Ferreira Team Leader Graduated in Biological Sciences with a BA in Genetics from the Federal University of Rio de Janeiro (1991), a PhD in Biological Sciences (Genetics) from the Federal University of Rio de Janeiro/Ghent University (Brazil and Belgium/1997) and a Post-Doctorate in Genetics of plant development at the California Institute of Technology - Caltech (USA/2001). Full Professor at the Department of Genetics at the Institute of Biology at the Federal University of Rio de Janeiro. Has experience in plant genetics, with emphasis on Plant Biotechnology, working mainly on the following topics such as control of gene expression, abiotic stress and functional genomics. Accredited advisor in the postgraduate courses in Genetics (level 7) and in Plant Biotechnology and Bioprocesses (level 6) at UFRJ. He has supervised 25 Master's dissertations, 13 doctoral theses and 10 postdoctoral supervisions. Since 2010, he has been working as a member of the CAPES Biotechnology Area Committee in the postgraduate evaluation. Contact Marcio Alves-Ferreira Plant Molecular Genetics and Biotechnology Laboratory Bloco A, A2-93 Institute of Biology, Department of Genetics Universidade Federal do Rio de Janeiro, Cidade Universitária - CEP: 21941-617, Rio de Janeiro, RJ, Brazil.

Learn more about AL13, its main goals within the project and meet the laboratory's work team. AL 13 - Field Phenotyping - Public Sector Laboratory Activities - PlantStress Biotech INCT Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Phenotyping (greenhouse or field) cotton population resulting from crossing with GM events to obtain proof of concept of drought tolerance and/or resistance to Spodoptera frugiperda , Helicoverpa armigera or Meloidogyne spp. Jaime Vasconcelos Cavalcanti Team Leader He has a degree in Agronomy from the Federal Rural University of Pernambuco (1987), master's degree in Agronomy (Genetics and Plant Improvement) from the Federal University of Lavras (1997) and a PhD in Molecular Biology - University of Reading, England (2004). He is currently a researcher at the Brazilian Agricultural Research Corporation, acting as coordinator of the Platform Project for the development of transgenic cotton plants resistant to the boll weevil at Embrapa Algodão. He is a permanent professor of postgraduate courses in plant genetic improvement at the Federal Rural University of Pernambuco (UFRPE) and agricultural sciences at the State University of Paraíba (UEPB), teaching, in both, the discipline of Quantitative Genetics. He has experience in the area of Agronomy, with an emphasis on plant genetic improvement, mainly on the following topics: genetic improvement of white and colored cotton, quantitative genetics, transgenics, molecular genetics and genomic statistics. Carlos Alberto D. da Silva Agronomist from the Faculty of Agronomy and Zootechnics Manoel Carlos Gonçalves (1986) holds a master's degree in Agronomy (Plant Protection) from the Universidade Estadual Paulista Júlio de Mesquita Filho (1992), a doctorate in Entomology from the Federal University of Viçosa (2006) and specialization in management by Fundação Dom Cabral (2009). Approved in the public competition of the Faculty of Agronomy of Vale do São Francisco (FAMESF) of the State University of Bahia (UNEB) on March 21, 1994, he served as assistant professor teaching plant health for a year, when he left to take on the position of researcher at Embrapa. He has been a researcher at Embrapa Algodão since December 22, 1994 to the present day. At this research institution he served as Deputy Head of Research and Development from March 2008 to July 2013. Invited to work as a professor of the postgraduate course in Agricultural Sciences at the State University of Paraíba (UEPB) in August 2010 teaches agricultural entomology within the discipline of plant health of bioenergetic plants. He coordinated, participated and participates in several research projects related to the integrated management of cotton pests in partnership with public and private companies in Brazil, in addition to collaborating with international cooperation projects with African countries. He has experience in the area of Agronomy, with an emphasis on Agricultural Entomology, working mainly with biological control and plant resistance to insects. Jose Ednilson Miranda Agronomist from the State University of Londrina (1995), Master's degree in Zootechnics (Animal Production Concentration Area) from the São Paulo State University Júlio de Mesquita Filho (1998) and PhD in Agronomy (Agricultural Entomology Concentration Area) from the Paulista State University Júlio de Mesquita Son (2001). Researcher at the Brazilian Agricultural Research Corporation since July 2002. He has experience in the area of Agricultural Entomology, working on the topics of integrated cotton pest management, biological control, insecticidal plants. Contact Jaime Vasconcelos Cavalcanti EMBRAPA Cotton Rua Oswaldo Cruz, n° 1.143, Bairro Centenário, CEP: 58428-095, Campina Grande, PB E-mail: jaime.cavalcanti@embrapa.br Phone:+55 83 3182 4300

Learn more about AL04, its main goals within the project and meet the laboratory's work team. AL 04 - Molecular Physiology Laboratory Activities - PlantStress Biotech INCT Sequencing and integrating transcriptomes of model plants (Arabdopsis , Setaria ) subjected to drought and high temperature during bioassays. Validate the function of plant genes potentially involved in the mechanisms of drought tolerance in Arabidopsis , rice or sepia plants via overexpression or silencing strategies. Generate PGMs from soybean, cotton and corn via strategies of overexpression or silencing of plant genes and evaluate the phenotype obtained regarding drought tolerance. Phenotyping (greenhouse or field) maize, soybean and cotton populations resulting from crossing GM events to obtain proof of concept of drought tolerance and/or resistance to Spodoptera frugiperda , Helicoverpa armigera or Meloidogyne spp. Generate PGMs from soybean, cotton and corn via strategies of overexpression or silencing of plant genes and evaluate the phenotype obtained regarding drought tolerance. Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Joaquim Albenisio Gomes da Silveira Team Leader Dr. Joaquim works with Plant Physiology, with emphasis on the efficiency of photosynthesis mediated by redox metabolism, photorespiration and N assimilation under conditions of abiotic stress. He holds a Master's degree from CENA and a doctorate from ESALQ/USP. He was a post-doctorate fellow at the Laboratory of Molecular Biology of Plant Stress at ITQB/Universidade Nova de Lisboa (2014-2015). He was one of the cofounders of the Brazilian Society of Plant Physiology, and was President of the Organizing Committee of the III Brazilian Congress of Plant Physiology. He participated in the creation and publication of the Brazilian Journal of Plant Physiology (1989), currently named Theoretical and Experimental Plant Physiology. Dr. Joaquim has published more than 150 scientic articles in international journals. He has trained over 29 doctorate students, 36 master students and has supervised 10 post-doctoral fellows. Nowadays, he is a professor at the Federal University of Ceará (UFC) anda a CNPq 1A researcher. He is also the vice-coordinator of the Graduate Program in Biochemistr at UFC and the head of Plant Metabolism Laboratory at the same university. Contact Joaquim Albenisio Gomes da Silveira Universidade Federal do Ceará Campus do Pici - Bloco 907 - CeP 60440-900 - Fortaleza - CE E-mail: silveira@ufc.br Phone number: +55 85 3366-9821