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Learn more about AL03, its main goals within the project and meet the laboratory's work team. AL 03 - Molecular Genetics Laboratory Activities - PlantStress Biotech INCT Integration of the transcriptome and sequencing of the model plant (Setaria) submitted to drought and high carbon dioxide content. Validate the expression of key genes in the metabolic pathways of plants response to combined stresses (biotic-biotic; biotic-biotic; abiotic-biotic) by qRT-PCR. Identify in silico promoters responsive to biotic and abiotic stress (drought and phytonematodes), with activity in different organs (root and leaf). Set up a vector bank with promoters directing the expression of reporter genes (GUS/GFP) in monocots and dicots. Validate promoters by transient transformation by biolistics or Agrobacterium rhyzogenes in target plants for in vivo testing of promoters (soybean, cotton and corn). Analyze the sequencing data to check the methylation status of gene promoters in the metabolic pathways of interest. Validate the function of plant genes potentially involved in drought tolerance mechanisms in Arabidopsis , rice or sepia plants through strategies of overexpression or silencing. Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Márcio Alves Ferreira Team Leader Graduated in Biological Sciences with a BA in Genetics from the Federal University of Rio de Janeiro (1991), a PhD in Biological Sciences (Genetics) from the Federal University of Rio de Janeiro/Ghent University (Brazil and Belgium/1997) and a Post-Doctorate in Genetics of plant development at the California Institute of Technology - Caltech (USA/2001). Full Professor at the Department of Genetics at the Institute of Biology at the Federal University of Rio de Janeiro. Has experience in plant genetics, with emphasis on Plant Biotechnology, working mainly on the following topics such as control of gene expression, abiotic stress and functional genomics. Accredited advisor in the postgraduate courses in Genetics (level 7) and in Plant Biotechnology and Bioprocesses (level 6) at UFRJ. He has supervised 25 Master's dissertations, 13 doctoral theses and 10 postdoctoral supervisions. Since 2010, he has been working as a member of the CAPES Biotechnology Area Committee in the postgraduate evaluation. Contact Marcio Alves-Ferreira Plant Molecular Genetics and Biotechnology Laboratory Bloco A, A2-93 Institute of Biology, Department of Genetics Universidade Federal do Rio de Janeiro, Cidade Universitária - CEP: 21941-617, Rio de Janeiro, RJ, Brazil.

Learn more about AL13, its main goals within the project and meet the laboratory's work team. AL 13 - Field Phenotyping - Public Sector Laboratory Activities - PlantStress Biotech INCT Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Phenotyping (greenhouse or field) cotton population resulting from crossing with GM events to obtain proof of concept of drought tolerance and/or resistance to Spodoptera frugiperda , Helicoverpa armigera or Meloidogyne spp. Jaime Vasconcelos Cavalcanti Team Leader He has a degree in Agronomy from the Federal Rural University of Pernambuco (1987), master's degree in Agronomy (Genetics and Plant Improvement) from the Federal University of Lavras (1997) and a PhD in Molecular Biology - University of Reading, England (2004). He is currently a researcher at the Brazilian Agricultural Research Corporation, acting as coordinator of the Platform Project for the development of transgenic cotton plants resistant to the boll weevil at Embrapa Algodão. He is a permanent professor of postgraduate courses in plant genetic improvement at the Federal Rural University of Pernambuco (UFRPE) and agricultural sciences at the State University of Paraíba (UEPB), teaching, in both, the discipline of Quantitative Genetics. He has experience in the area of Agronomy, with an emphasis on plant genetic improvement, mainly on the following topics: genetic improvement of white and colored cotton, quantitative genetics, transgenics, molecular genetics and genomic statistics. Carlos Alberto D. da Silva Agronomist from the Faculty of Agronomy and Zootechnics Manoel Carlos Gonçalves (1986) holds a master's degree in Agronomy (Plant Protection) from the Universidade Estadual Paulista Júlio de Mesquita Filho (1992), a doctorate in Entomology from the Federal University of Viçosa (2006) and specialization in management by Fundação Dom Cabral (2009). Approved in the public competition of the Faculty of Agronomy of Vale do São Francisco (FAMESF) of the State University of Bahia (UNEB) on March 21, 1994, he served as assistant professor teaching plant health for a year, when he left to take on the position of researcher at Embrapa. He has been a researcher at Embrapa Algodão since December 22, 1994 to the present day. At this research institution he served as Deputy Head of Research and Development from March 2008 to July 2013. Invited to work as a professor of the postgraduate course in Agricultural Sciences at the State University of Paraíba (UEPB) in August 2010 teaches agricultural entomology within the discipline of plant health of bioenergetic plants. He coordinated, participated and participates in several research projects related to the integrated management of cotton pests in partnership with public and private companies in Brazil, in addition to collaborating with international cooperation projects with African countries. He has experience in the area of Agronomy, with an emphasis on Agricultural Entomology, working mainly with biological control and plant resistance to insects. Jose Ednilson Miranda Agronomist from the State University of Londrina (1995), Master's degree in Zootechnics (Animal Production Concentration Area) from the São Paulo State University Júlio de Mesquita Filho (1998) and PhD in Agronomy (Agricultural Entomology Concentration Area) from the Paulista State University Júlio de Mesquita Son (2001). Researcher at the Brazilian Agricultural Research Corporation since July 2002. He has experience in the area of Agricultural Entomology, working on the topics of integrated cotton pest management, biological control, insecticidal plants. Contact Jaime Vasconcelos Cavalcanti EMBRAPA Cotton Rua Oswaldo Cruz, n° 1.143, Bairro Centenário, CEP: 58428-095, Campina Grande, PB E-mail: jaime.cavalcanti@embrapa.br Phone:+55 83 3182 4300

Learn more about AL08, its main goals within the project and meet the laboratory's work team. AL 08 - Plant Transformation Laboratory Activities - PlantStress Biotech INCT Identify in silico promoters responsive to biotic and abiotic stress. Clone promoters responsive to drought and phytonematodes with activity in different organs (root and leaf). Validate promoters by transient transformation by biolistics or Agrobacterium rhyzogenes in target plants for in vivo testing of promoters (soybean, cotton and corn). Validate promoters by stable transformation in target plants for in vivo testing of promoters identified as promising in previous tests (soybeans, cotton and corn). Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Generate GM soybean, cotton and corn via strategies of overexpression or silencing of plant genes and evaluate the phenotype obtained regarding drought tolerance. Generate GM soybean and cotton via strategies of overexpression or silencing of plant genes and evaluate the phenotype obtained for resistance to nematodes. Generate GM soybean, cotton and corn via toxin overexpression strategies and evaluate the phenotype obtained regarding the control of Helicoverpa armigera and Spodoptera frugiperda . Laboratory Description Research efforts in our laboratory have focused on prospecting and characterization of genes involved in plant response to biotic and abiotic stresses. Emphasis: Soybean (Glycine max ), Jackbean (Canavalia ensiformis ) and Solanum nigrum . Approaches: Gene expression analysis; Gene silencing; Overexpression; Subcellular localization of gene products. For genetic transformation, we use mainly particle bombardment, but we are gaining experience on Agrobacterium -mediated transformation. Embryogenic tissues are used as targets for transformation. Research Lines Characterization of soybean genes involved in flood and drought tolerance. Overexpression of Jaburetox and Soyuretox, urease-derived peptides, for plant protection against insects, fungi and nematodes. Characterization of WRKY soybean genes responsive to water deficit and study of their promoters. • Expression of a gene that encodes an osmotin, isolated from Solanum nigrum, aiming enhanced drought tolerance in soybean. Our Team Maria Helena Bodanese Zanettini Team Leader She holds a degree in Natural History from the Federal University of Rio Grande do Sul (1972), a master's degree in Genetics and Molecular Biology from the Federal University of Rio Grande do Sul (1975) and a PhD in Genetics and Molecular Biology from the Federal University of Rio Grande do Sul ( 1982). Retired Full Professor at the Department of Genetics at UFRGS. She is currently Professor/Advisor of the Graduate Program in Genetics and Molecular Biology at the same University. Has experience in the field of Genetics, with an emphasis on Plant Genetics, working mainly in the lines of research: 1) Prospection of genes, tissue culture and genetic manipulation of plants; 2) Genetics, evolution and conservation of plants. Interested in the following topics: soy; somatic embryogenesis; anther culture; genetic transformation; biolistics; Agrobacterium system; co-transformation; transgenic plants; functional genomics; insect resistance; fungus resistance; genetic variability; population structure; plant cytogenetics; meiotic behavior; playback mode; fertility. Christian Bredemeier Graduated in Agronomy from the Federal University of Rio Grande do Sul (UFRGS), Master in Plant Science from UFRGS and PhD in Plant Nutrition/Precision Agriculture from the Technical University of Munich - Weihenstephan (Freising, Germany). Currently is Associate Professor at the Department of Crop Plants / UFRGS and Professor at the Graduate Program in Phytotechnics at UFRGS and at the Graduate Program in Precision Agriculture at the Federal University of Santa Maria (UFSM). Coordinates the Digital Agriculture Study Group (GEAD / UFRGS) and is a founding partner of the Brazilian Institute of Bioeconomy (INNBIO). Currently, is President of the Brazilian Commission for Precision and Digital Agriculture - CBAPD/MAPA (Period 2021-2023), Scientific Deputy Director of the Brazilian Association for Precision and Digital Agriculture - AsBraAP (Period 2020-2024), Member of the Chamber of Agro 4.0 and Member of the Network of Mentors in Innovation at ZENIT - Scientific and Technological Park of UFRGS. He was Coordinator of the Graduate Program in Phytotechnics / UFRGS, in the period 2017-2021. Areas of expertise: Precision Agriculture and Digital Agriculture, variable rate nitrogen fertilization, ecophysiology and crop plant management, sensors, remote sensing and unmanned aerial vehicles (UAVs). Contact Maria Helena Bodanese Zanettini Universidade Federal do Rio Grande do Sul Campus do Vale, Bento Gonçalves Avenue 9500, Building 4342212, Postal Code 91501-910, Porto Alegre, RS, Brazil E-mail: maria.zanettini@ufrgs.br Phone number: +55 51 3008-6725

Meet the researchers who coordinate the INCT PlantSTress Biotech. The Team Maria Fatima Grossi-de-Sa Coordinator Ph.D - Researcher Embrapa Genetic Resources Biotechnology (Embrapa Cenargen). Expertise: Plant biotechnology; Plant genetic engineering; Functional genomics; Insect nanobiotechnology; Plant genome editing; Heterologous proteins. Info Rogério Margis Vice-coordinator Ph.D - Full Professor Department of Biophysics and Biotechnology Center, Universidade Federal do Rio Grande do Sul (UFRGS). Expertise: Plant molecular biology; Interfering RNA; Biotechnology. Joaquim Albenísio G. Silveira Vice-coordinator Ph.D - Full Professor Departament of Biochemistry and Molecular Biology, Universidade Federal do Ceará (UFC). Expertise: Plant molecular physiology; Plant biochemistry; Water stress; abiotic stresses. Management Committee Maria Fatima Grossi-de-Sa (Coordinator): Ph.D - Researcher, Embrapa Genetic Resources Biotechnology (Embrapa Cenargen); Expertise: Plant biotechnology; Plant genetic engineering; Functional genomics; Insect nanobiotechnology; Plant genome editing; Heterologous proteins. Ana Cristina Miranda Brasileiro: Ph.D - Researcher, Embrapa Genetic Resources Biotechnology (Embrapa Cenargen); Expertise: Plant molecular biology; Functional genomics; Plant genetic transformation. Antonio Costa de Oliveira: Ph.D - Full Professor, Genetic Department, Federal University of Pelotas (UFPEL); Expertise: Functional and structural genomics; Molecular breeding. Patrícia Messenberg Guimarães: Ph.D - Researcher, Embrapa Genetic Resources Biotechnology (Embrapa Cenargen); Expertise: Molecular genetics; Plant molecular biology; Functional genomics. Márcio Alves Ferreira: Ph.D - Full Professor; Genetic Department, Federal University of Rio de Janeiro (UFRJ); Expertise: Plant genomics, Biotechnology.

Learn more about AL05, its main goals within the project and meet the laboratory's work team. AL 05 - Plant-Pest Interaction Laboratory Activities - PlantStress Biotech INCT Sequencing transcriptomes associated with the response to drought of native species in Brazil (wild peanuts, pitangueira, Clúsia; and cashew) by large-scale sequencing. Identify and select in silico candidate genes related to drought tolerance of native species (wild peanuts, pitangueira, Clúsia, and cashew). Identify InDels and SNPs in candidate genes of native species associated with the drought response (wild peanuts, pitangueira, and cashew). Validate in vitro the expression profile of candidate genes for tolerance to drought obtained from native species in Brazil (wild peanuts, pitangueira, Clúsia, and cashew). Select vital genes/molecules for gall nematodes (Meloidogyne spp.) by analyzing their genome. Select potential genes involved in resistance from contrasting genotypes (peanuts, soybean, rice, cotton and coffee). Integrate legume transcriptome data (beans, soybean, and peanuts) submitted to water deficit, generated by mass sequencing (Illumina – HiSeq) in previous projects. Integrate transcriptome data from resistant genotypes (beans, soybean, rice, coffee, and peanuts) infected by nematodes, generated by mass sequencing (Illumina – HiSeq) in previous projects. Sequencing and integrating transcriptome of drought tolerant genotypes of Musa spp., Arachis spp., and cowpea subjected to water deficit combined with biotic stress (Meloidogyne spp. or Mycosphaerella ) in bioassays. Validate the expression of key genes in the metabolic pathways of plants' response to combined stresses (biotic-biotic; biotic-biotic; abiotic-biotic) by qRT-PCR. Sequencing on the Illumina platform a fraction of small RNAs, and their target mRNAs, and circular RNAs of plants (Arachis ; Musa ; soybean; pitangueira; cashew tree) subjected to biotic and/or abiotic stresses. Analyze sequencing data to check methylation status of gene promoters in the metabolic pathways of interest. Validate the function of plant genes potentially involved in the mechanisms of drought tolerance in Arabidopsis , rice or sepia plants via overexpression or silencing strategies. Validate the function of plant genes potentially involved in nematode resistance mechanisms via overexpression or silencing strategies. Validate the function of nematode genes potentially involved in the parasitism mechanisms, via gene silencing strategies in model systems. Assess the potential of biotechnological assets generated for the purpose of intellectual protection. Patent the use of genes and gene elements validated during the project Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Laboratory Description The Plant-Pest Interaction Laboratory conducts studies on the interaction of plants with biotic and abiotic stresses, aiming at elucidating the changes in the cellular, biochemical, physiological and molecular machinery of plants, which occur in response to different stresses, combined or not. In addition to prospecting and identifying genes, regulatory sequences and molecules involved in plant tolerance/resistance responses to one or more stresses, research group performs the validation of function of these assets in model plants and methods are developed and improved for the validation of these assets in target plants, which will enable the development of cultivars more adapted to different environmental conditions. Research Lines Prospecting for genes/molecules or peptides of interest for the control of drought and pests in wild peanut germplasm (Arachis spp.). Prospecting of target molecules in phytoparasitic nematodes (Meloidogyne spp.) for pest control. Prospecting for small RNAs in plant genotypes that are resistant/tolerant to pests and drought that may be involved in these stresses. Prospecting for molecules and peptides that are efficient in controlling pests and tolerating water deficit simultaneously (cross-stress). Vallidation of assets due to their overexpression or gene silencing in model plants for analysis and validation of their function. Our Team Patrícia Messemberg Guimarães Team Leader Graduated in Agronomy from the University of Brasília (1985), master's degree in Phytopathology from the University of Brasília (1987) and doctorate in Molecular Biology - University of London (1997). She did post-doctorate in plant genomics at CIRAD (France) in 2006. She is currently a collaborator at the University of Brasilia (Unb) and Catholic University of Brasilia (UCB) and researcher at the Brazilian Agricultural Research Corporation since 1989. The main research areas include Biochemistry, Molecular Biology, Plant-pathogen interaction, and works mainly on the following themes: structural and functional genomics of legumes, Arachis genomics, plant resistance, plant tolerance to water stress, genetic maps, and molecular characterization of plants. She is coordinator of several national and international projects in the field of genetics and genomics of legumes and plant-pest interaction. Ana Cristina Miranda Brasileiro Graduated in Forestry Engineering from the University of Brasília (1986), Master in Molecular and Plant Cell Biology - Universite de Paris XI (Paris-Sud) (1988) and PhD in Molecular and Plant Cell Biology - Universite de Paris XI (Paris-Sud ) (1992). He is currently a researcher at the Brazilian Agricultural Research Corporation. From 2002 to 2006 she worked as a researcher at Labex-Europa at Cirad (France). He has experience in the field of Genetics, with an emphasis on Plant Genetics, working mainly on the following themes: genetic transformation, plant biotechnology, Agrobacterium biology, gene expression and genomics. Since 2006, she has been coordinator in Brazil of the International Consortium in Advanced Biology (CIBA), an initiative of Agropolis (France) and Embrapa (Brazil), whose objective is to create and consolidate an efficient strategy of international scientific and technical cooperation, to study and explore the diversity in genetic resources of plants and to identify important genes and characteristics essential for genetic improvement programs in Tropical and Mediterranean agriculture. From 2008 to 2014 he participated as an external member of the Scientific Council of the Department of Biological Systems (BIOS) of Cirad/France and since 2016 he has been an external member of the Scientific and Strategic Council of Cirad/France. Ana Claudia Guerra de Araújo Researcher Ana Claudia Guerra de Araujo has a degree in Biology from the University of Brasília (1987), a PhD in Biological Sciences (Biophysics) from the Federal University of Rio de Janeiro (1994). Since 1994 she is a Researcher at Embrapa, at Cenargen, where she has been working with the interface between cellular and plant molecular biology at the Microscopy Laboratory. He has a post-doctorate in Australia (CSIRO, 2001) where he worked with molecular and cellular techniques in plant reproduction and in England (University of Leicester, 2011), where he worked with plant molecular cytogenetics using the microscopy tool. At Embrapa, he develops research in the area of plant reproduction, through studies of morphology, cell biology and ultrastructure of developmental biology, involving techniques of cytochemistry, immunocytochemistry, cytogenetics, in situ hybridization associated with microscopy. It is also involved in studies on the plant-pathogen interaction, responses to biotic and abiotic stresses and quality in Arachis, determining factors for the success of a productive and sustainable agriculture. André Southernman Teixeira Irsigler Graduated in Biological Sciences (2000), Master (2002) and Doctorate (2007) in Genetics and Breeding from Universidade Federal de Viçosa, with a sandwich period at North Carolina State University, and Post-doctorate at Florida State University. He is a researcher at Embrapa Genetic Resources and Biotechnology, and is involved in the areas of regulation of gene expression and developmental biology. Contact Patricia Messenberg Guimarães EMBRAPA Genetic Resources and Biotechnology W5 Norte Avenue (end) - P.O. Box 02372 - Postal Code 70770-917 - Brasília, DF - Brazil E-mail: patricia.guimaraes@embrapa.br Phone:+55 61 3448-4787

Here you can find all registered patents. Results of works carried out by the INCT PlantStress Biotech network. Publications Patents RELEVANT PATENTS (FROM 2017) Application Patents / Grant Patents / Licensed Patents ALVES-FERREIRA, M, Conforte, AJ, Dias, FG, Malato, MB, Grossi-de-Sa, MF, Nepomuceno, AL (2018). Compositions and Methods for Modifying Gene Expression Using Water Deficit-Inducible Soybean Promoter in Plants. BR1020180037692. Instituição de registro: INPI - Instituto Nacional da Propriedade Industrial. BRASILEIRO, ACM, Guimaraes, PM, Araújo, ACG, Lacorte, CC (2018) Método para Produção de Plantas com Tolerância a Estresse Abiótico e com Resistência a Pragas. BR102018003666-1. Instituição de registro: INPI - Instituto Nacional da Propriedade Industrial. FREITAS-SILVA, MV, Barreto-Bergter, E, Silva-Neto MAC, Montebianco, CB, Bernardino, MC, Mattos, B,Carvalho, SS (2017). Composição Praguicida, Uso, Método de Controle de Patógenos de Plantas, Método de Controle de Propagação de Insetos e Processo de Obtenção de Proteínas de Fungos. BR10201700204. Instituição de registro: INPI – Instituto Nacional da Propriedade Industrial. FREITAS-SILVA, MV, Côrrea, RL, Vidal, MS, Barroso, PAV. Teste Molecular Voltado para a Identificação e o Diagnóstico In Vitro do Vírus Responsável pela Doença Azul do Algodoeiro (CLRDV). PI06001513. Instituição de registro: INPI - Instituto Nacional da Propriedade Industrial. Concessão: 15/08/2017 . GUIMARÃES, LM, Batista, JAN, Viana, AMB, Fragoso, RR, Silva, MCM, Grossi-de-Sa, MF. Composições e Métodos para Modificar a Expressão de Genes Usando o Promotor do Gene da Proteína de Conjugação Ubiquitina de Plantas de Soja. INPI -Data do Depósito: 05/02/2007. CARTA PATENTE Nº PI0701172-5, concessão: 26/11/2019 . GROSSI-DE-SA, MF, Silva, MCM, Del Sarto, RP, Rocha, TL. Mutantes de Inibidores de Alfa Amilases Isolados de Phaseolus vulgaris com propriedades de Controlar Insetos-Praga, Composições Contendo Tais Mutantes e Métodos de Obtenção dos Mesmos e de Linhagens Transgênicas. INPI-Brazil: PI1102841-6, Deposito: 08/06/2011. CARTA PATENTE Nº PI 1102841-6. Concessão: 21/11/2018 . GROSSI-DE-SA, M.F.; Grossi-de-Sa, M.F; Silva, M. C. M.; Fonseca, F. C. A.; Macedo, L. L. P.; Lourenco, I. T.; Albuquerque, E. V. S. (2013). Aparato e método de criação de larvas de insetos em laboratório. Patent application, BR1020130331120, 20/12/2013, INPI-Brazil. CARTA PATENTE Nº BR102013033112-0. Concessão: 24/11/2020 . GROSSI-DE-SA, MF, Guimarães, LM, Batista, JAN, Viana, AAB, Fragoso, RR, Rocha, T (2007). Composições e métodos para modificar a expressão de genes usando o promotor do gene da proteína de Conjugação à ubiquitina de plantas de Algodão. 2007, Brasil. INPI – Brazil, PI07012306, Deposito: 05/02/2007, Publication Date: 23/09/2008. CARTA PATENTE Nº PI 0701230-6 B1. Concessão: 26/06/2018 . GROSSI-DE-SA, MF, Macedo, LLP. Silva, MCM, Almeida Garcia, R, Silva, LP, Vila A (2018). Pesticide, European Patent Office. WO2020007450, PCT/EP2018/067922. Patent Application: 03/07/2018. GROSSI-DE-SA, MF, Brasileiro, ACM, Guimarães, PM, Morgante, CV, Lourenço-Tessutti, IT, Arraes, FBM, Pereira, BM (2022). High Throughput Soybean Root Disease Screen. US Patent Application WO2024073346. filled Patent: 26/09/2022. GROSSI-DE-SA, MF, Macedo, LLP, Pinto, CLM, Leite, AGB, Silva, MCM, Lourenço-Tessutti, IT, Morgante, CV (2020). Método para Produzir Planta Resistente a Inseto Praga e Moléculas de Ácido Nucleico Utilizadas para Obtenção de Tal Planta Através de dsRNAs Relacionados a Ecdise. PI1020200043129 A2. Instituição de registro: INPI - Instituto Nacional da Propriedade Industrial. GROSSI-DE-SA, MF, Macedo, LLP, Salles-Filho, ALO, Silva, MCM, Lourenço-Tessuti, IT, Morgante, CV (2025). Biocomplexos para modificação genética in planta. 2025, Brasil. Patente: Privilégio de Inovação. Número do registro: BR10202500402. INPI - Instituto Nacional da Propriedade Industrial. Depósito: 28/02/2025. GROSSI-DE-SA, MF, Silva, MCM, Macedo, LLP, Lourenço-Tessutti, IT, Morgante, CV, Leite, AGB (2021). Silenciamento do gene para a proteína caderina para aplicação no controle do inseto-praga, Anthonomus grandis, via RNA interferente. 2021, Brasil.Patente: Privilégio de Inovação. Número do registro: BR1020210115300, Instituição de registro: INPI - Instituto Nacional da Propriedade Industrial. Depósito: 14/06/2021. Hemerly, AS, Cavalcanti, PF, Gong P, Nelissen H, Inze D, Grossi-de-Sa, MF, Basso, MF, Morgante, CV, Lisei-de-Sa, ME (2020). Method to increase plant biomass, plant yield and plant drought tolerance. Continuation in part (CIP) patent application US†20160177327†A1. United States Patent and Trademark Office Patent Filling: 04/05/2020. Patent Grant: 13/12/2022. Licensed Patent: 2023 – INARI SEEDesign Company. MACEDO, LLP, Grossi-de-Sa, MF, SILVA, MCM, GARCIA, RAG, Freire, EVSA (2017). Aumento da Eficácia de Supressão de Expressão de Genes por Meio do Uso de Molécula de RNA com Estrutura Estabilizada. BR1020170069044. Instituição de registro: INPI - Instituto Nacional da Propriedade Industrial. REMER, R. A.; Margis, R.; Lima, M. C.; Coronha, L. M.; Alves-Ferreira. Produto Farmacêutico e Processo para sua Produção. PI03051978. Instituição de registro: INPI – Instituto Nacional da Propriedade Industrial. Depósito: 13/11/2003; Concessão: 02/04/2019 . ROCHA-LIMA, T, Evaristo, RGS, Grossi de Sa, MF, Silva, MCM, Polez, VLP, ROESSNER, U, Bacic, T. (2017). Nematotoxic composition of synergistic effect. use of a nematotoxic composition of synergistic effect. United States Patent and Trademark Office. US9750247B2. Patent Application: 26/12/2012; PCT Application: 26/12/2013; Patent Grant: 05/09/2017 . ROCHA-LIMA, T, Grossi-de-Sa, MF, Silva, MCM, Polez, VP, Evaristo, RGS, Roessner, Um, Bacic, T (2013). Composição nematotoxica de efeito sinergico, uso de composição nematotóxica de efeito sinérgico. 2013, Brasil.Patente: Privilégio de Inovação. Número do registro: BR0020130095109, Instituição de registro: INPI - Instituto Nacional da Propriedade Industrial. Depósito: 26/12/2013; Depósito PCT: 26/12/2013; Concessão: 03/11/2021 . GROSSI-DE-SA, MF, Silva, MCM, Gomes Jr, JE, Lourenco, IT, Macedo LLP, Lucena WA, Fonseca, FCA (2013). Moléculas variantes sintéticos de toxinas Cry1IA12 com propriedades de controlar insetos-praga, composições contendo tais mutantes e método de utilização dos mesmos: BR102012033542-5 A2, concessão: 24/08/2021 . Synthetic variant molecules of toxins Cry1IA12 with properties to control pest insects, compositions containing such mutants and method of use of the same: AR094338A1, concessão: 01/12/2023 . Instituição de registro: WIPO - World Intellectual Property Organization. CARNEIRO, NP, Almeida-Carneiro, A, Hercos Valicente, F, Martins Mendes S, Alvs, MC, Neto Parentoni, S (2024). Method for the control of populations of insect pests resistant to the VIP3AA Protein. INPI BR102023004777A2 (14/03/2023), PCT PCT/BR2024/050091 (13/03/2024), WO2024187259 (18/09/2024). Carneiro, NP, Valicente, FH, Carneiro, AA, Noda, RW, Meire,CA, Barros, BA (2018). Molécula de ácido nucleico cry1DA com códons otimizados, construções de DNA, vetor, célula hospedeira, célula vegetal, planta transgênica, métodos de transformação e de controle de pragas (Pedido de patente BR1020180092638. Instituto Nacional da Propriedade Industrial – INPI.

Seminars e Lectures Plant and Animal Genome Conference 2023 (PAG 30) 13 a 18 de janeiro de 2023 – San Diego/EUA Integrated Omics Data Unravel Soybean Mechanisms Associated with Root-Knot Nematode Tolerance 53º Congresso Brasileiro de Fitopatologia 7 a 10 de agosto de 2023 – Brasília/DF RNAi-mediated parasitism gene silencing as a new source of resistance against the root-knot nematode International Congress of Plant Pathology 2023 20 a 25 de agosto de 2023 – Lyon/França RNAi-mediated parasitism gene silencing as source of crop protection resistance to Meloidogyne incognita 68º Congresso Brasileiro de Genética 12 a 15 de setembro de 2023 – Ouro Preto/MG RNAi technology: Developing RNAi-based solutions for crop plants Simpósio e Diplomação de Novos Membros Afiliados da ABC 2023-2027 – Região Norte 27 de outubro de 2023 – Palmas/TO Palestra Magna – RNA Technology: challenges and developing RNAi-based solutions for crop plants (From Bench to Market: developed biotech products) VIII Simpósio Brasileiro de Genética Molecular de Plantas 30 de maio a 2 de junho de 2023 – Florianópolis/SC Organização da sessão (Chair) Applied Plant Biotechnology International Plant & Animal Genome 31 - PAG 2024 San Diego on January 12-17, 2024- San Diego, CA, USA Palestra: RNAi-mediated down-regulation of the endogenous aip10 genes in transgenic cotton (Gossypium hirsutum ) events improves earliness, productivity, and drought tolerance XXIX Congresso Brasileiro de Entomologia e XIII CLE RNAi approach for insect pest control: Advances, applications, and Challenges 22 a 26 de setembro de 2024- Uberlandia – MG Palestra: RNAi approach for insect pest control: Advances, applications, and Challenges International Plant Molecular Biology (IPMB) 24-28 de junho de 2024 – Queensland- Australia Palestra: RNAi for insect pest control: Transgenic and topical approaches AgroBrasilia 2024 20-24 de maio de 2024 Brasilia-DF-Brasil Palestra: Controle Biológico de Pragas e Doenças na sua lavoura: Tecnologia de interferência de RNA (RNAi) para melhoramento de plantas e controle de pragas ICE2024 Kyoto- XXVII International Congress of Entomology 25-30 de Agosto de 2024 Palestra: RNAi and Bt approaches for insect pest control: advances, applications, and challenges International Plant & Animal Genome 32 - PAG 2025 San Diego on January 10-15, 2025 - San Diego, CA, USA Palestra: An Ex Vitro Soybean Hairy Root System for Screening Target Molecules Applied in Biotech Approaches

Learn more about AL15, its main goals within the project and meet the laboratory's work team. AL 16 - Biotechnological Applications of Microorganisms Laboratory Description - PlantStress Biotech INCT Virus:plant interaction and the identification of viruses associated with cotton diseases through sequencing and characterization of new viruses, development of molecular diagnostic tests, transcriptome and miRNA profile during viral infection. The main lines are the characterization of the molecular mechanism of resistance to CBD; the use of fungal molecules as inducers of protection against viral diseases; and the expression of SARS-CoV-2 antigens in plants. Research Lines Cell Biology, Biochemistry and Genetics of Microorganisms Biotechnological Applications of Microorganisms and their Products Microorganisms/Virus-Host Interactions Our Team Maite Vaslin de Freitas Silva Team Leader Associate Professor IV, member of PPG-Micro and PPG-PBV at UFRJ. Director of the Brazilian Society of Virology. Master's and PhD in Genetics from UFRJ in molecular biology of plants. Postdoc at IB UFRJ studying RNAi in antiviral defense. Visiting professor IB UFRJ 199-2001 and permanent professor at IMPG since 2002. I coordinate the Plant Molecular Virology Laboratory, whose lines are: the study of the virus: plant interaction and the identification of viruses associated with cotton diseases through sequencing and characterization of new viruses, development of molecular diagnostic tests, transcriptome and miRNA profile during viral infection. Today the main lines are the characterization of the molecular mechanism of resistance to CBD; the use of fungal molecules as inducers of protection against viral diseases; and the expression of SARS-CoV-2 antigens in plants. The group has 2 patents filed and 1 granted and is encouraging the birth of startups based on the research developed. Contact Maite Vaslin de Freitas Silva Federal University of Rio de Janeiro - UFRJ Department of Virology – Paulo de Góes Institute of Microbiology – UFRJ E-mail: maite@micro.ufrj.br Phone:+55 21 2560 8344

Learn more about AL01, its main goals within the project and meet the laboratory's work team. AL 01 - Genomics and Proteomics Laboratory Activities - PlantStress Biotech INCT Integration data from monocotyledon transcriptome (rice, corn, wheat, Musa and sorghum) submitted to water deficit, generated by mass sequencing (Illumina - HiSeq) in previous projects. Integration of transcriptome data from drought-tolerant genotypes of Musa spp., Arachis spp. and cowpea subjected to water deficit combined with biotic stress (Meloidogyne spp. or Mycosphaerella ) in bioassays. Integration and sequencing of transcripts of fungus-tolerant genotypes in environments subjected to multiple stresses (Musa X Mycosphaerella musicola and Fusarium oxysporun ). Validation of key genes expression in the metabolic pathways of plants' response to combined stresses (biotic-biotic; biotic-biotic; abiotic-biotic) by qRT-PCR. Small RNAs, mRNAs and circular plant RNAs sequencing from Arachis , Musa , soybean, pitangueira, and cashew, previously subjected to biotic and/or abiotic stresses, using the Illumina platform. Validation of plant genes function potentially involved in drought tolerance mechanisms in Arabidopsis , rice or Setaria plants by overexpression or silencing strategies. Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Laboratory Description The Genomics and Proteomics Laboratory studies the prospection and identification of new genes, regulatory sequences and molecules involved with the resistance/tolerance responses of plants to biotic and abiotic stresses, aiming at the elucidation of the molecular mechanisms of plants that occur in response to different stresses, combined or not. The research group also performs fucntion validation of these assets in model plants. Research Lines Prospecting genes/molecules of interest for pest and drought control in wild germplasm of Musa spp. Prospecting target molecules in phytoparasitic nematodes (Meloidogyne spp.) and phytopathogenic fungi (Pseudocercospora spp.) for pest control. Prospecting for small RNAs in plant genotypes that are resistant/tolerant to pests and drought that may be involved in responding to these stresses. Prospecting for genes/molecules that are efficient in pest control and tolerance to water deficit simultaneously (cross-stress). Validation of prospective innovation assets by their overexpression or gene silencing in model plants for analysis and validation of their function. Robert Neil Gerard Miller Team Leader Graduated in Biological Sciences - Manchester Metropolitan University, UK (1990), Master in Plant Protection - University Of Bath, UK (1991) and PhD in Molecular Biology and Phytopathology - University of Reading, UK (1995). He is currently Associate Professor I at the University of Brasilia (Campus Darcy Ribeiro, Department of Cellular Biology), supervising the Graduate Programs in Molecular Biology, Phytopathology and Microbial Biology. He worked between 2014 and 2016 as Coordinator of the Graduate Program in Molecular Biology (CAPES concept 6). He works as editor for the journals Annals of Botany and Tropical Plant Pathology, he is coordinator of national and international projects, mainly in the following themes: functional genomics of plants and microorganisms; search for genes for resistance to biotic stress in plants; and characterization of phytopathogenic and mycotoxigenic fungi. Contact Robert Neil Gerard Miller Microbiology Laboratory: Plant-Prague Interaction, Bloco I-1-35/8, Institute of Biological Sciences, Department of Cell Biology, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Asa Norte, Postal Code 70910-900, Brasília, DF, Brazil.

Learn more about AL07, its main goals within the project and meet the laboratory's work team. AL 07 - Insects and Nematodes: Breeding and Bioassays Laboratory Activities - PlantStress Biotech INCT Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Leonardo Lima Pepino de Macedo Team Leader Dr. Leonardo is undergraduated in Biological Sciences from the Federal University of Rio Grande do Norte (2005). He holds a master's degree in Biochemistry from the Federal University of Rio Grande do Norte (2007), and a doctorate's degree in Genomic Science and Biotechnology from the Catholic University of Brasília (2012). He has experience in Biochemistry and Molecular Biology, with expertise in the following topics: cloning and expression of proteins in heterologous systems; bioprospecting of proteins with entomotoxic activity (vicillins, lectins, proteinase inhibitors and Cry toxins) aiming at the control of dipterous, lepidopteran and coleopteran insects; and development of gene silencing strategies via interfering RNA (RNAi) for the control of insect pests. Contact Leonardo Lima Pepino de Macedo EMBRAPA Genetic Resources and Biotechnology W5 Norte Avenue (end) - P.O. Box 02372 - Postal Code 70770-917 - Brasília, DF - Brazil E-mail: leonardo.lima@embrapa.br Phone number:+55 61 3448-4705

Find here the events organized by INCT PlantStress Biotech and participate! Events Organization Workshops with the INCT-PlantStress-Biotech team , held in February/2022. Member of international committee XXVIII Plant and Animal Genome Conference , San Diego, CA, EUA, Antônio C. de Oliveira, held from 11 to 18 January 2020. Member of international committee ISRFG 2019 , Taipei, Taiwan, Antônio C. de Oliveira, held from 4 to 6 November 2019. Workshops with the INCT-PlantStress-Biotech team , held in April/2017, December/2018 and May/2019. Organization of the 7th Brazilian Congress of Biotechnology , organized by SBBIOTEC (Brazilian Society of Biotechnology), whose current president is Dr. Maria Fatima Grossi-de-Sá, held from 18 to 21 November 2018.

Learn more about AL12, its main goals within the project and meet the laboratory's work team. AL 12 - Colaboration Lab Laboratory Activities - PlantStress Biotech INCT Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Carmen S. Soares Pires Team Leader Dr. Carmen is undergraduated in Biology from the Federal University of Viçosa (1984), holds a master's degree in Entomology from the same university and a Ph.D in Biology fomr the Northern Arizona University (1998). She has been a researcher at Embrapa Genetic Resources and Biotechnology since 1989. She has experience in Ecology, with amphasis on Insect Ecology, working mainly o the following topics: population dynamics, plant-insect pest-natural enemies interaction, conservative biological control and pollination in agroecosystems. Since 2003, she has been involved in environmental risk analysis projects of stressors on non-target organisms (predatory insects, pollinators and non-target herbivores). Angharad MR Gatehouse Team Leader Prof Angharad Gatehouse has the Chair of Invertebrate Molecular Biology at Newcastle University and is Director of Expertise for BioEconomy. Her research focuses on the molecular and biochemical bases of plant-pest interactions with a view to developing novel strategies for crop protection. Her group was one of the first to produce insect-resistant transgenic crops. More recently her group has been using functional genomics to better understand the molecular responses of crops to biotic stress (pathogens and insect pests) for the development of molecular markers. In collaboration with Durham University, her group are actively involved in developing novel biopesticides including those based on RNAi. In addition to their efficacy, the group is also involved in the biosafety of these strategies, notably in terms of their potential effects on beneficial insects such as pollinators and natural enemies. She has published extensively in the field, including in discovery journals (Nature, PNAS). Contact Carmen S. Soares Pires Embrapa - Recursos Genéticos e Biotecnologia Parque Estação Biológica - PqEB, s/n, Brasília - DF carmen.pires@embrapa.br +55 61 3448-4433 Angharad MR Gatehouse School of Natural and Environmental Sciences - Newcastle University, Ridley Building. a.m.r.gatehouse@ncl.ac.uk +44 (0) 191 208 8838