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Embrapa Genetic Resources and Biotechnology. Parque Estação Biológica - PqEB - W5 Norte Avenue (end) P.O. Box 02372 - Brasília, DF - Brazil - Postal Code 70770-917. Phone number: +55 61 3448-4902 Contact Coordinator: Maria Fatima Grossi-de-Sa Embrapa Genetic Resources and Biotechnology Parque Estação Biológica - PqEB - W5 Norte Avenue (end) P.O. Box 02372 - Brasília, DF - Brazil - Postal Code 70770-917 Phone number: +55 61 3448-4902 E-mail: fatima.grossi@embrapa.br /inctplantstresssite@gmail.com You can also contact us by filling this contact form: First Name Last Name Email Phone Subject Message Submit Thanks for submitting!

Get to know all the 15 associated laboratories (AL) who develop INCT PlantStress Biotech projects. Associated Laboratories (ALs) AL 01- Genomics and Proteomics Team Leader: Robert N. G. Miller (UnB) Partner Institutions: UnB, Embrapa Cenargen AL 02 - Transcriptomics, Epigenetics and Functional Genomics Team Leader: Rogério Margis (UFRGS) Partner Institutions: UFRGS, UFRJ, Embrapa Cenargen AL 03 - Molecular Genetics Team Leader: Márcio Alves-Ferreira (UFRJ) Partner Institutions: UFRJ, Embrapa Cenargen AL 04 - Molecular Physiology Team Leader: Joaquim A. G. Silveira (UFC) Partner Institutions: UFC, UFPel, Embrapa Clima Temperado AL 05 - Plant-Pest Interaction Team Leader: Patrícia Messenberg Guimarães (Embrapa Cenargen) Partner Institutions: Embrapa Cenargen, Embrapa Soja, Embrapa Cerrados, Embrapa Arroz e Feijão, Embrapa Milho e Sorgo AL 06 - Bioinformatics Roberto Coiti Togawa (Embrapa Cenargen) Partner Institutions: Embrapa Cenargen, UnB, UFPel AL 07 - Insects-Nematodes: Creation and Bioassays Team Leader: Leonardo Pepino (Embrapa Cenargen) Partner Institutions: Embrapa Cenargen, Embrapa Soja, Embrapa Milho e Sorgo AL 08 - Plant Transformation - Soybean Team Leader: Maria Helena Zanettini (UFRGS) Partner Institutions: UFRGS AL 09 - Plant Transformation - Cotton Team Leader: Maria Fátima Grossi-de-Sa (Embrapa Cenargen) Partner Institutions: Embrapa Cenargen AL 10 - Plant Transformation - Corn Team Leader: Newton Carneiro (Embrapa Milho e Sorgo) Partner Institutions: Embrapa Milho e Sorgo AL 11 - Biometrics Team Leader: Antônio C. de Oliveira (UFPel) Partner Institutions: UFPel AL 12 - Evaluation of Environmental Risks Carmen Pires (Embrapa Cenargen) Angharad Gatehouse (Newcastle University) Partner Institution: Embrapa Cenargen e Newcastle University AL 13 - Field Phenotyping - Public Sector Team Leader: Jaime Cavalcanti (Embrapa Algodão) Partner Institutions: Embrapa Soja, Embrapa Milho e Sorgo, Embrapa Algodão, Embrapa Clima Temperado AL 14 - Field Phenotyping - Private Sector Team Leader: Rafael Galbieri (IMAmt) Partner Institutions: IMAmt - Instituto Matogrossense do Algodão AL 15 - Molecular Plant-Pathogen Interaction Team Leader: Francismar C. Marcelino Guimarães (Embrapa Soja) Partner Institutions: Embrapa Soja AL 16 - Biotechnological Applications of Microorganisms Team Leader: Maite Vaslin de Freitas Silva (UFRJ) Partner Institutions: Federal University of Rio de Janeiro

Find here the events organized by INCT PlantStress Biotech and participate! Events Organization Workshops with the INCT-PlantStress-Biotech team , held in February/2022. Member of international committee XXVIII Plant and Animal Genome Conference , San Diego, CA, EUA, Antônio C. de Oliveira, held from 11 to 18 January 2020. Member of international committee ISRFG 2019 , Taipei, Taiwan, Antônio C. de Oliveira, held from 4 to 6 November 2019. Workshops with the INCT-PlantStress-Biotech team , held in April/2017, December/2018 and May/2019. Organization of the 7th Brazilian Congress of Biotechnology , organized by SBBIOTEC (Brazilian Society of Biotechnology), whose current president is Dr. Maria Fatima Grossi-de-Sá, held from 18 to 21 November 2018.

Learn more about AL12, its main goals within the project and meet the laboratory's work team. AL 12 - Colaboration Lab Laboratory Activities - PlantStress Biotech INCT Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Carmen S. Soares Pires Team Leader Dr. Carmen is undergraduated in Biology from the Federal University of Viçosa (1984), holds a master's degree in Entomology from the same university and a Ph.D in Biology fomr the Northern Arizona University (1998). She has been a researcher at Embrapa Genetic Resources and Biotechnology since 1989. She has experience in Ecology, with amphasis on Insect Ecology, working mainly o the following topics: population dynamics, plant-insect pest-natural enemies interaction, conservative biological control and pollination in agroecosystems. Since 2003, she has been involved in environmental risk analysis projects of stressors on non-target organisms (predatory insects, pollinators and non-target herbivores). Angharad MR Gatehouse Team Leader Prof Angharad Gatehouse has the Chair of Invertebrate Molecular Biology at Newcastle University and is Director of Expertise for BioEconomy. Her research focuses on the molecular and biochemical bases of plant-pest interactions with a view to developing novel strategies for crop protection. Her group was one of the first to produce insect-resistant transgenic crops. More recently her group has been using functional genomics to better understand the molecular responses of crops to biotic stress (pathogens and insect pests) for the development of molecular markers. In collaboration with Durham University, her group are actively involved in developing novel biopesticides including those based on RNAi. In addition to their efficacy, the group is also involved in the biosafety of these strategies, notably in terms of their potential effects on beneficial insects such as pollinators and natural enemies. She has published extensively in the field, including in discovery journals (Nature, PNAS). Contact Carmen S. Soares Pires Embrapa - Recursos Genéticos e Biotecnologia Parque Estação Biológica - PqEB, s/n, Brasília - DF carmen.pires@embrapa.br +55 61 3448-4433 Angharad MR Gatehouse School of Natural and Environmental Sciences - Newcastle University, Ridley Building. a.m.r.gatehouse@ncl.ac.uk +44 (0) 191 208 8838

Learn more about AL06, its main goals within the project and meet the laboratory's work team. AL 06 - Bioinformatics Laboratory Activities - PlantStress Biotech INCT Select vital genes/molecules for gall nematodes (Meloidogyne spp.) by analyzing their genome. Select potential genes involved in resistance from contrasting genotypes (peanuts soybean, rice, cotton and coffee). Integrate legume transcriptome data (beans, soybean and peanuts) submitted to water deficit, generated by mass sequencing (Illumina – HiSeq) in previous projects. Integrate monocotyledonous transcriptome data (rice, corn, wheat, Musa and Sorghum ) submitted to water deficit, generated by mass sequencing (Illumina – HiSeq) in previous projects. Integrate transcriptome data from resistant genotypes (beans, soybean, rice, coffee and peanuts) infected by nematodes, generated by mass sequencing (Illumina – HiSeq) in previous projects. Sequence on the Illumina platform a fraction of small RNAs, and their target mRNAs, and circular RNAs of plants (Arachis , Musa, soybean, pitangueira and cashew tree) subjected to biotic and/or abiotic stresses. Analyze sequencing data to check methylation status of gene promoters in the metabolic pathways of interest. Organize an in silico database cured and shared by INCT members. Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Our Team Roberto Coiti Togawa Team Leader Graduated in Data Processing from the University of Brasília (1984) and doctorate from the University of Bedfordshire (United Kingdom) (2006). He worked for several years in the area of basic software support and systems development. He is currently a research analyst at Embrapa Genetic Resources and Biotechnology. Has experience in Computer Science, with emphasis on Programming Languages, working mainly on the following topics: Development of analysis tools for genomic sequences, Analysis of NGS sequences. Development of analysis tools for protein structures, structural bioinformatics, Development of prediction and analysis tools for Membrane Proteins. Priscila Grynberg She holds a degree in Biological Sciences from the Federal University of Minas Gerais - UFMG (2004), a master's degree in Parasitology from UFMG (2007) and a PhD in Bioinformatics from UFMG (2011). She is currently Researcher A at Embrapa Genetic Resources and Biotechnology - Cenargen. Operates in research projects of functional analysis of gene expression and differential proteomics data, large-scale sequencing of microRNAs and data mining. In 2007, she was awarded the Samuel Pessoa Medal at the 20th Brazilian Congress of Parasitology, when she won first place with a work that associated non-synonymous polymorphisms in the apical membrane protein 1 of Plasmodium vivax (PvAMA-1) with the platelet count in patients infected. She is part of the executive committee of the Brazilian Association of Bioinformatics and Computational Biology (AB3C). Contact Roberto Coiti Togawa EMBRAPA Genetic Resources and Biotechnology W5 Norte Avenue (end) - P.O. Box 02372 - Postal Code 70770-917 - Brasília, DF - Brazil E-mail: roberto.togawa@embrapa.br

Learn more about AL09, its main goals within the project and meet the laboratory's work team. AL 09 - Plant Transformation - Cotton Laboratory Activities - PlantStress Biotech INCT Searching for vital insect-pest genes/molecules (Helicoverpa armigera and Spodoptera frugiperda ), using large-scale sequencing of their transcriptome and in vitro validation of gene expression. S election of new Cry molecules with high toxic activity against S. frugiperda and H. armigera . In silico identification of target molecules against insects and nematodes, through the design of specific chemical drugs for the development of new insecticides and nematicides. Analysis of the genome of Meloidogyne spp. for the selection of genes/molecules vital for gall formation. Selection of potential genes involved in the resistance of contrasting genotypes (peanuts, soybean, rice, cotton, and coffee). Integration of monocotyledonous transcriptome data (rice, corn, wheat, Musa spp., and Sorghum spp.) submitted to water deficit, generated by mass sequencing (Illumina – HiSeq) in previous projects. Integration of transcriptome data from drought-tolerant genotypes from Musa spp., Arachis spp., and cowpea submitted to water deficit combined with biotic stress (Meloidogyne spp. or Mycosphaerella ) in bioassays. Use of qRT-PCR for validation of key genes expression from metabolic pathways related to plant responses to combined stresses (biotic-biotic, biotic-biotic, abiotic-biotic). Search and validation of novel regulatory sequences (promoters) responsive to biotic and abiotic stresses, in crop plants, using transient and stable transformation (soybeans, cotton, and maize). Sequencing and selection of plant small RNAs, mRNAs, and circular RNAs (Arachis spp. , Musa spp. , soybean, pitangueira, cashew tree) submitted to biotic and/or abiotic stresses, using the Illumina platform. Validation of plant genes function, potentially involved in nematode resistance mechanisms, through molecules overexpression or gene silencing (RNAi) strategies. Validation of nematode genes function, potentially involved in the parasitism mechanisms, through RNAi strategies in model systems. Assessment and monitoring technology of biotech assets prospected for intellectual protection. Development of universal vectors containing all prospected and patented genetic elements by different institutions from the INCT Project. National and international protection of genes and regulatory sequences, via patents. Developing of GM soybean, cotton, and maize plants through overexpression of molecules and/or gene silencing strategies for drought tolerance and nematode resistance. Developing of GM soybean, cotton, and corn plants through overexpressing Bt toxin and dsRNAs sequences applied to H. armigera and S. frugiperda control. Functional validation of multiple pyramided genes involved in multiple traits, including nematodes and insect-pests (S. frugiperda or H. armigera ) resistance and drought tolerance in GM soybean and cotton plants. Phenotyping (greenhouse and/or field simulation) GM maize, soybean, and cotton plants for drought tolerance and/or resistance to S. frugiperda , H. armigera and Meloidogyne spp. Laboratory Description The Plant-Pest Molecular Interaction Laboratory (LIMPP) is coordinated by Dr. Maria Fatima Grossi-de-Sa. Research interests include both basic and applied sciences, focusing on plant-pest molecular interactions (pathogens and insect-pests) to develop novel crop protection strategies, especially for cotton and soybean. The LIMPP group’s research is known for its expertise in plant biotechnology using functional genomics, notably working on biotechnological aspects of RNA interfering (RNAi) mechanism applied to insect-pests and phytonematodes. Other current research interests include exploring new technologies for plant genetic transformation and genome editing, novel regulatory sequences for genetic engineering of crop plants for protection against insect-pests and phytonematodes, drought tolerance, and development of recombinant proteins. Research Lines Search for novel genes/molecules and peptides to be applied on the control of cotton and soybean insect-pests. Search for target genes/molecules of plant parasitic nematodes (Meloidogyne spp., Rotylenchus reniformis , Aphelenchoides spp.) to be applied in gene silencing approaches. Genetic engineering of crop plants using genome editing technologies and target genes/molecules of contrasting soybean genotypes (resistant/susceptible to Meloidogyne spp.). Search for genes/molecules and small RNAs in pest-resistance and drought-tolerant plant genotypes potentialy involved in the response to biotic and abiotic stresses (cross-stress). Validation of novel prospected biotech assets using overexpression of molecules or gene silencing approaches in plant models. Genetic engineering development of crop plants (cotton, soybean, sugarcane) using molecules/dsRNAs overexpression, gene silencing, and genome editing approaches for traits that include pest control, increase in biomass, and drought tolerance. Recent Publications ATELLA, A. L.; Grossi-de-Sa, M. F.; Alves-Ferreira, M. (2023). Cotton promoters for controlled gene expression. Electronic Journal of Biotechnology, v. 62, p. 10.1016/j.ejbt. https://doi.org/10.1016/j.ejbt.2022.12.002 BASSO, M. F.; Lourenço-Tessutti, I. T.; Moreira-Pinto, C. E.; Mendes, R. A. G.; Pereira, D. G.; Grandis, A.; Macedo, L. L. P.; Macedo, A. F.; Gomes, A. C. M. M.; Arraes, F. B. M.; Togawa, R. C.; do Carmo Costa, M. M.; Marcelino-Guimaraes, F. C.; Silva, M. C. M.; Floh, E. I. S.; Buckeridge, M. S., de Almeida Engler, J., Grossi-de-Sa, M. F. (2023) . Overexpression of the GmEXPA1 gene reduces plant susceptibility to Meloidogyne incognita . Plant Cell Report , v. 42, n. 1, p. 137-152. https://doi.org/10.1007/s00299-022-02941-3 FONSECA, F. C. A.; Antonino, J. D.; de Moura, S. M.; Rodrigues-Silva, P. L.; Macedo, L. L. P.; Gomes Júnior, J. E.; Lourenço-Tessuti, I. T.; Lucena, W. A.; Morgante, C. V.; Ribeiro, T. P.; Monnerat, R. G.; Rodrigues, M. A.; Cuccovia, I. M.; Mattar Silva, M. C.; Grossi-de-Sa, M. F. (2023) . In vivo and in silico comparison analyses of Cry toxin activities toward the sugarcane giant borer. Bulletin of Entomological Research, v. 8, p. 1-12. https://doi.org/10.1017/S000748532200061X MOREIRA, V. J. V.; Pinheiro D. H.; Lourenço-Tessuti, I. T.; Basso, M. F. ; Lisei-de-Sá, M. E.; Silva, M. C. M.; Danchin, E. G. J. ; Guimarães, P. M.; Grynberg, P.; Brasileiro, A. C. M.; Macedo, L. L. P.; Morgante, C. V.; Engler, J. A.; Grossi-de-Sá, M. F. (2023) . In planta RNAi targeting Meloidogyne incognita Minc16803 gene perturbs nematode parasitism and reduces plant susceptibility. Journal of Pest Science , v. 1, p. 1. https://doi.org/10.1007/s10340-023-01623-7 NIZOLLI, V. O.; Oliveira, V. F.; Maia, L. C.; Pegoraro, C.; Oliveira, A. C. (2023) . Genome editing in rice: New paths for an old crop. Perspectives In Agriculture, Veterinary Science, Nutrition And Natural Resources, v. 2023, p. 1-8. http://dx.doi.org/10.1079/cabireviews.2023.0008 PEREIRA, B. M.; Arraes, F.; Martins, A. C. Q.; Alves, N. S. F.; Melo, B. P.; Morgante, C. V.; Saraiva, M. A. P.; Grossi-de-Sá, M. F.; Guimarães, P. M.; Brasileiro, A. C. M. (2023). A novel soybean hairy root system for gene functional validation. PLoS One, v. 18, p. e0285504. https://doi.org/10.1371/journal.pone.0285504 TRENZ, T. S.; Turchetto-Zolet, A. C.; Margis, R.; Margis-Pinheiro, M.; Maraschin, F. S. (2023) . Functional analysis of alternative castor bean DGAT enzymes. Genetics and Molecular Biology (Online Version), v. 46, p . 1-12. https://doi.org/10.1590/1678-4685-GMB-2022-0097 VASQUEZ, D. D. N.; Pinheiro, D. H.; Teixeira, L. A.; Moreira-Pinto, C. E.; Macedo, L. L. P.; Salles-Filho, A. L. O.; Silva, M. C. M.; Lourenço-Tessutti, I. T.; Morgante, C. V.; Silva, L. P.; Grossi-de-Sa, M. F. (2023). Simultaneous silencing of juvenile hormone metabolism genes through RNAi interrupts metamorphosis in the cotton boll weebil. Frontiers in Molecular Biosciences, v. 10, p. 1073721. https://doi.org/10.3389/fmolb.2023.1073721 ARRAES, F. B. M.; Vasquez, D. D. N.; Tahir, M.; Pinheiro, D. H.; Faheem, M.; Freitas-Alves, N. S.; Moreira-Pinto, C. E.; Moreira, V. J. V.; Paes-de-Melo, B.; Lisei-de-Sá, M. E.; Morgante, C. V.; Mota, A. P. Z.; Lourenço-Tessutti, I. T.; Togawa, R. C.; Grynberg, P.; Fragoso, R. R.; de Almeida-Engler, J.; Larsen Martin, R.; Grossi-de-Sa, M. F. (2022) . Integrated Omic Approaches Reveal Molecular Mechanisms of Tolerance during Soybean and Meloidogyne incognita Interactions. Plants , v. 11, p. 2744. https://doi.org/10.3390/plants11202744 . BASSO, M. F.; Lourenço-Tessutti, I. T.; Moreira-Pinto, C. E.; Mendes, R. A. G.; Pereira, D. G.; Grandis, A.; Macedo, L. L. P.; Macedo, A. F.; Gomes, A. C. M. M.; Arraes, F. B. M.; Togawa, R. C.; do Carmo Costa, M. M.; Marcelino-Guimarães, F. C.; Silva, M. C. M.; Floh, E. I. S.; Buckeridge, M. S.; de Almeida-Engler, J.; Grossi-de-Sa, M. F. (2022) . Overexpression of the GmEXPA1 gene reduces plant susceptibility to Meloidogyne incognita . Plant Cell Reports , v. 12, p. s00299-022-0294. https://doi.org/10.1007/s00299-022-02941-3 . BASSO, M. F.; Lourenço-Tessutti, I. T.; Moreira-Pinto, C. E.; Mendes, R. A. G.; Paes-de-Melo, B.; Das Neves, M. R.; Macedo, A. F.; Figueiredo, V.; Grandis, A.; Macedo, L. L. P.; Arraes, F. B. M.; do Carmo-Costa, M. M.; Togawa, R. C.; Enrich-Prast, A.; Marcelino-Guimarães, F. C.; Gomes, A. C. M. M.; Silva, M. C. M.; Floh, E. I. S.; Buckeridge, M. S.; de Almeida-Engler, J.; Grossi-de-Sa, M. F. (2022) . Overexpression of a soybean Globin (GmGlb1-1) gene reduces plant susceptibility to Meloidogyne incognita . Planta , v. 256, p. 83. https://doi.org/10.1007/s00425-022-03992-2 . DE MOURA, S. M.; Babilonia, K.; de Macedo, L. L. P.; Grossi-de-Sa, M. F.; Shan, L.; He, P.; Alves-Ferreira, M. (2022) . The oral secretion from Cotton Boll Weevil ( Anthonomus grandis ) induces defense responses in cotton ( Gossypium spp) and Arabidopsis thaliana . Current Plant Biology , v. 31, p. 100250. https://doi.org/10.1016/j.cpb.2022.100250 . DE MOURA, S. M.; Freitas, E. O.; Ribeiro, T. P.; Paes-de-Melo, B.; Arraes, F. B. M.; Macedo, L. L. P.; Paixão, J. F. R.; Lourenço-Tessutti, I. T.; Artico, S.; da Cunha Valença, D.; Silva, M. C. M.; de Oliveira, A. C.; Alves-Ferreira, M.; Grossi-de-Sa, M. F. (2022) . Discovery and functional characterization of novel cotton promoters with potential application to pest control. Plant Cell Reports , v. 41, p. 10.1007/s00299. https://doi.org/10.1007/s00299-022-02880-z. DOS SANTOS, C.; Carmo, L. S. T.; Távora, F. T. P. K.; Lima, R. F. C.; da Nobrega Mendes, P.; Labuto, L. B. D.; de Sá M. E. L.; Grossi-de-Sa, M. F.; Mehta, A. (2022) . Overexpression of cotton genes GhDIR4 and GhPRXIIB in Arabidopsis thaliana improves plant resistance to root-knot nematode ( Meloidogyne incognita ) infection. 3 Biotech , v. 12, p. 211. https://doi.org/10.1007/s13205-022-03282-4 . FRAGOSO, R. R.; Arraes, F. B. M.; Lourenço-Tessutti, I. T.; Miranda, V. J.; Basso, M. F.; Ferreira, A. V. J.; Viana, A. A. B.; Lins, C. B. J.; Lins, P. C.; Moura, S. M.; Batista, J. A. N.; Silva, M. C. M.; Engler, G.; Morgante, C. V.; Lisei-de-Sá, M. E.; Vasques, R. M.; de Almeida-Engler, J.; Grossi-de-Sa, M. F. (2022) . Functional characterization of the pUceS8.3 promoter and its potential use for ectopic gene overexpression. Planta , v. 256, p. 69, 2022. https://doi.org/10.1007/s00425-022-03980-6 . KARALIJA, E.; Vergata, C.; Basso, M. F.; Negussu, M.; Zaccai, M.; Grossi-de-Sa, M. F.; Martinelli, F. (2022) . Chickpeas? Tolerance of Drought and Heat: Current Knowledge and Next Steps. Agronomy-Basel , v. 12, p. 2248. https://doi.org/10.3390/agronomy12102248 . MENDES, R. A. G.; Basso, M. F.; Amora, D. X.; Silva, A. P.; Paes-de-Melo, B.; Togawa, R. C.; Albuquerque, E. V. S.; Lisei-de-Sá, M. E.; Macedo, L. L. P.; Lourenço-Tessutti, I. T.; Grossi-de-Sa, M. F. (2022) . In planta RNAi approach targeting three M. incognita effector genes disturbed the process of infection and reduced plant susceptibility. Experimental Parasitology , v. 238, p. 108246. https://doi.org/10.1016/j.exppara.2022.108246 . MOREIRA, V. J. V.; Lourenço-Tessutti, I. T.; Basso, M. F.; Lisei-de-Sá, M. E.; Morgante, C. V.; Paes-de-Melo, B.; Arraes, F. B. M.; Martins-de-Sa, D.; Silva, M. C. M.; de Almeida-Engler, J.; Grossi-de-Sa, M. F. (2022) . Minc03328 effector gene downregulation severely affects Meloidogyne incognita parasitismo in transgenic Arabidopsis thaliana . Planta , v. 255, p. 44-59. https://doi.org/10.1007/s00425-022-03823-4 . REIS, M. A.; Noriega, D. D.; dos Santos Alves, G.; Coelho, R. R.; Grossi-de-Sa, M. F.; Antonino, J. D. (2022) . Why is oral-induced RNAi inefficient in Diatraea saccharalis ? A possible role for DsREase and other nucleases. Pesticide Biochemistry and Physiology , v. 186, p. 105166. https://doi.org/10.1016/j.pestbp.2022.105166 . RIBEIRO, D. G.; Mota, A. P. Z.; Santos, I. R.; Arraes, F. B. M.; Grunberg, P.; Fontes, W.; de Souza Castro, M.; de Sousa, M. V.; Lisei-de-Sá, M. E.; Grossi-de-Sa, M. F.; Franco, O. L.; Mehta, A. (2022) . NBS-LRR-WRKY genes and protease inhibitors (PIs) seem essential for cowpea resistance to root-knot nematode. Journal of Proeomics , v. 261, p. 104575. https://doi.org/10.1016/j.jprot.2022.104575 . RIBEIRO, T. P.; Vasquez, D. D. N.; Macedo, L. L. P.; Lourenço-Tessutti, I. T.; Valença, D. C.; Oliveira-Neto, O. B.; Paes-de-Melo, B.; Rodrigues-Silva, P. L.; Firmino, A. A. P.; Basso, M. F.; Lins, C. B. J.; Neves, M. R.; Moura, S. M.; Tripode, B. M. D.; Miranda, J. E.; Silva, M. C. M.; Grossi-de-Sa, M. F. (2022) . Stabilized Double-Stranded RNA Strategy Improves Cotton Resistance to CBW (Anthonomus grandis ). International Journal of Molecular Sciences , v. 23, p. 13713. https://doi.org/10.3390/ijms232213713 . TOUZDJIAN PINHEIRO KOHLRAUSCH TÁVORA, F.; de Assis dos Santos Diniz, F.; de Moraes Rêgo-Machado, C.; Chagas Freitas, N.; Barbosa Monteiro Arraes, F.; Chumbinho de Andrade, E.; Furtado, L. L.; Osiro, K. O.; Lima de Sousa, N.; Cardoso, T. B.; Márcia Mertz Henning, L.; Abrão de Oliveira Molinari, P.; Feingold, S. E.; Hunter, W. B.; Grossi-de-Sa, M. F.; Kobayashi, A. K.; Lima Nepomuceno, A.; Santiago, T. R.; Correa Molinari, H. B. (2022) . CRISPR/Cas- and Topical RNAi-Based Technologies for Crop Management and Improvement: Reviewing the Risk Assessment and Challenges Towards a More Sustainable Agriculture. Frontiers in Bioengineering and Biotechnology , v. 10, p. 10:913728. https://doi.org/10.3389/fbioe.2022.913728 . ARAUJO SOUSA, B.; Nascimento Silva, O.; Farias Porto, W.; Lima Rocha, T.; Paulino Silva, L.; Ferreira Leal, A.P.; Buccini, D.F.; Oluwagbamigbe Fajemiroye, J.; de Araujo Caldas, R.; Franco, O.L.; Grossi-De-Sá, M.F.; de La Fuente Nunez, C.; Moreno, S.E. (2021) . Identification of the active principle conferring anti inflammatory and antinociceptive properties in bamboo plant. Molecules , v. 26, p. 3054. https://doi.org/10.3390/molecules2610305 . ARRAES, F.B.M.; Martins-de-Sa, D.; Noriega Vasquez, D.D.; Melo, B.P.; Faheem, M.; de Macedo, L.L.P.; Morgante, C.V.; Barbosa, J.A.R.G.; Togawa, R.O.; Moreira, V.J.P.; Danchin, E.G.J.; Grossi-de-Sa, M.F. (2021) . Dissecting protein domain variability in the core RNA interference machinery of five insect orders. RNA Biology , v. 18, p. 1653-1681. https://doi.org/10.1080/15476286.2020.1861816 . BASSO, M.F.; Costa, J.A.; Ribeiro, T.P.; Arraes, F.B.M.; Lourenço-Tessutti, I.T.; Macedo, A.F.; Neves, M.R.; Nardeli, S.M.; Arge, L.W.; Perez, C.E.A.; Silva, P.L.R; De Macedo, L.L.P.; Lisei-de-Sa, M.E.; Amorim, R.M.A.; Pinto, E.R.C.; Silva, M.C.M.; Morgante, C.V.; Floh, E.I.S.; Alves-Ferreira, M.; Grossi-de-Sa, M.F. (2021) . Overexpression of the CaHB12 transcription factor in cotton ( Gossypium hirsutum ) improves drought tolerance. Plant Physiology and Biochemistry , v. 165, p. 80-93. https://doi.org/10.1016/j.plaphy.2021.05.009 . CABRAL, D.; Forero Ballesteros, H.; de Melo, B.P.; Lourenço-Tessutti, I.T.; Smões de Siqueira, K.M.; Obicci, L.; Grossi-de-Sa, M.F.; Hemerly, A.S.; de Almeida Engler, J. (2021) . The armadillo BTB protein ABAP1 is a crucial player in DNA replication and transcription of nematode-induced galls. Frontiers in Plant Science , v. 12, p. 636663. https://doi.org/10.3389/fpls.2021.636663. GODINHO MENDES, R.A.; Basso, M.F.; Fernandes de Araújo, J.; Paes De Melo, B.; Lima, R.N.; Ribeiro, T.P.; da Silva Mattos, V.; Saliba Albuquerque, E.V.; Grossi-De-Sa, M.; Dessaune Tameirao, S.N.; da Rocha Fragoso, R.; Mattar da Silva, M.C.; Vignols, F.; Fernandez, D.; Grossi-De-Sa, M.F. (2021) . Minc00344 and Mj-NULG1a effectors interact with GmHub10 protein to promote the soybean parasitism by Meloidogyne incognita and M. javanica . Experimental Parasitology , v. 229, p. 108153. https://doi.org/10.1016/j.exppara.2021.108153 . LISEI-DE-SÁ, M.E.; Rodrigues-Silva, P.L.; Morgante, C.V.; de Melo, B.P.; Lourenço-Tessutti, I.T.; Arraes, F.B.M.; Sousa, J.P.A.; Galbieri, R.; Amorim, R.M.S.; de Lins, C.B.J.; Macedo, L.L.P.; Moreira, V.J.; Ferreira, G.F.; Ribeiro, T.P.; Fragoso, R.R.; Silva, M.C.M.; de Almeida-Engler, J.; Grossi-de-Sa, M.F. (2021) . Pyramiding dsRNAs increases phytonematode tolerance in cotton plants. Planta , v. 254, p. 121. https://doi.org/10.1007/s00425-021-03776-0 . MENDES, R. A. G.; Basso, M. F.; Paes-de-Melo, B.; Ribeiro, T. P.; Lima, R. N.; Araujo, J. F.; Grossi-de-Sa, M. F.; Mattos, V. S.; Togawa, R. C.; Albuquerque, E. V. S.; Lisei-de-Sá, M. E.; Silva, M. C. M.; Macedo, L. L. P.; Fragoso, R. R.; Fernandez, D.; Vignols, F.; Grossi-de-Sa, M. F. (2021) . The Mi-EFF1/Minc17998 effector interacts with the soybean GmHub6 protein to promote host plant parasitism by Meloidogyne incognita . Physiological and Molecular Plant Pathology , v. 114, p. 101630. https://doi.org/10.1016/j.pmpp.2021.101630 . MENDES, R.A.G.; Basso, M.F.; Paes-de-Melo, B.; Ribeiro, T.P.; Lima, R.N.; Araujo, J.F.; Grossi-de-Sa, M.; Mattos, V.S.; Togawa, R.C.; Albuquerque, E.V.S.; Lisei-de-Sa, M.E.; Silva, M.C.M.; Macedo, L.L.P.; Fragoso, R.R.; Fernandez, D.; Vignols, F.; Grossi-de-Sa, M.F. (2021) . The Mi-EFF1/Minc17998 effector interacts with the soybean GmHub6 protein to promote host plant parasitism by Meloidogyne incognita . Physiological and Molecular Plant Pathology , v. 114, p. 101630. https://doi.org/10.1016/j.pmpp.2021.101630 . MOREIRA-PINTO, C.E.; Ramos Coelho, R.; Borges Leite, A.G.; Amaral Silveira, D.; Aguiar Souza, D.; Biaggioni Lopes, R.; Macedo, L.L.P.; Mattar Silva, M.C.; Ribeiro, T.P.; Morgante, C.V.; Antonino, J.D.; Grossi-de-Sa, M.F. (2021) . Increasing susceptibility to through-induced knockdown: a perspective to combine biocontrol and biotechnology. Pest Management Science , v. 77, p. ps.6430. https://doi.org/10.1002/ps.6430. MOREIRA-PINTO, C.E.; Coelho, R.R.; Leite, A.G.B.; Silveira, D.A.; Souza, D.A.; Lopes, R.B.; Macedo, L.L.P.; Silva, M.C.M.; Ribeiro, T.P.; Antonino, J.D.; Grossi-de-Sa, M.F. (2021) . Increasing Anthonomus grandis susceptibility to Metarhizium anisopliae through RNAi-induced AgraRelish knockdown: a perspective to combine biocontrol and biotechnology. Pest Management Science , v. 77, p. 4054-4063. https://doi.org/10.1002/ps.6430 . MOTA, A.P.Z.; Brasileiro, A.C.M.; Vidigal, B.; Oliveira, T.N.; da Cunha Quintana Martins, A.; Saraiva, M.A.P.; de Araújo, A.C.G.; Togawa, R.C.; Grossi-de-Sá, M.F.; Guimaraes, P.M. (2021) . Defining the combined stress response in wild Arachis. Scientific Reports , v. 11, p. 11097. https://doi.org/10.1038/s41598-021-90607-7 . PAES DE MELO, B.; Lourenço-Tessutti, I.T.; Fraga, O.T.; Pinheiro, L.B.; de Jesus Lins, C.B.; Morgante, C.V.; Engler, J.A.; Reis, P.A.B.; Grossi-De-Sá, M.F.; Fontes, E.P.B. (2021) . Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses. Scientific Reports , v. 11, p. 11178. https://doi.org/10.1038/s41598-021-90767-6. PAES DE MELO, B.; Moura, S.M.; Morgante, C.V.; Pinheiro, D.H.; Alves, N.S.F.; Rodrigues-Silva, P.L.; Lourenço-Tessutti, I.T.; Andrade, R.V.; Fragoso, R.R.; Grossi-de-Sa, M.F. (2021) . Regulated promoters applied to plant engineering: an insight over promising soybean promoters under biotic stress and their cis-elements. Biotechnology Research and Innovation , v. 5, p. e2021005. http://dx.doi.org/10.4322/biori.202105 . RIBEIRO, T.P.; Lourenço-Tessutti, I.T.; De Melo, B.P.; Morgante, C.V.; Filho, A.S.; Lins, C.B.J.; Ferreira, G.F.; Mello, G.N.; Macedo, L.L.P.; Lucena, W.A.; Silva, M.C.M.; Oliveira-Neto, O.B.; Grossi-de-Sa, M.F. (2021) . Improved cotton transformation protocol mediated by Agrobacterium and biolistic combined-methods. Planta , v. 254, p. 20. https://doi.org/10.1007/s00425-021-03666-5 . RODRIGUES-SILVA, P. L.; Rodrigues, M. T.; Figueiredo, L. H. M.; Grossi-de-Sa, M. F. (2021) . Tendências quanto ao conhecimento e às aplicações biotecnológicas do Psidium guineense evidenciadas pelo monitoramento tecnológico. Cadernos de Ciência & Tecnologia , v. 38, p. e26704. http://dx.doi.org/10.35977/0104-1096.cct2021.v38.26704 . BASSO, M.F.; Arraes, F.B.M.; Grossi-de-Sa, M.; Vaz-Moreira, V.J.; Alves-Ferreira, M.; Grossi-de-Sa, M.F. (2020) . Insights into genetic and molecular elements for transgenic crop development. Frontiers in Plant Science , v. 11, p. 509. https://doi.org/10.3389/fpls.2020.00509 . BASSO, M.F.; Lourenço-Tessutti, I.T.; Busanello, C.; Pinto, C.E.M.; Oliveira-Freitas, E.; Ribeiro, T.P.; Almeida-Engler, J.; Oliveira, A.C.; Morgante, C.V.; Alves-Ferreira, M.; Grossi-de-Sa, M.F. (2020) . Insights obtained using different modules of the cotton uceA1.7 promoter. Planta , v. 251, p. 56. https://doi.org/10.1007/s00425-020-03348-8 . BASSO, M.F.; Lourenço-Tessutti, I.T.; Mendes, R.A.G.; Pinto, C.E.M.; Bournaud, C.; Gillet, F.X.; Togawa, R.C.; Macedo, L.L.P.; Almeida-Engler, J.; Grossi-de-Sa, M.F. (2020) . MiDaf16-like and MiSkn1-like gene families are reliable targets to develop biotechnological tools for the control and management of Meloidogyne incognita . Scientific Reports , v. 10, p. 6991. https://doi.org/10.1038/s41598-020-63968-8 . BEVITORI, R.; Sircar, S.; Mello, R.N.; Togawa, R.C.; Cortes, M.V.C.B.; Oliveira, T.S.; Grossi-de-Sa, M.F.; Parekh, N. (2020) . Identification of co-expression gene networks controlling rice blast disease during an incompatible reaction. Genetics Molecular Research , v. 19, p. gmr18579. https://doi.org/10.4238/gmr18579 . CABRAL, D.N.; Banora, M.Y.; Antonino, J.D.; Rodiuc, N.; Vieira, P.; Coelho, R.R.; Chevalier, C.; Eekhout, T.; Engler G.; De-Veylder, L.; Grossi-de-Sa, M.F.; Almeida-Engler, J. (2020) . The plant WEE1 kinase is involved in checkpoint control activation in nematode-induced galls. New Phytologist , v. 225(1), p. 430-447. https://doi.org/10.1111/nph.16185 . CAMPOS, M.L.; Prado, G.S.; Santos, V.O.; Nascimento, L.C.; Dohms, S.M.; Cunha, N.B.; Ramada, M.H.S.; Grossi-de-Sa, M.F.; Dias, S.C. (2020) . Mosses: versatile plants for biotechnological applications. Biotechnology Advances , v. 6, p. 107533. https://doi.org/10.1016/j.biotechadv.2020.107533 . FIRMINO, A.A.P.; Pinheiro, D.H.; Pinto, C.E.M.; Antonino, J.D.; Macedo, L.L.P.; Martins-de-Sa, D.; Arraes, F.B.M.; Coelho, R.R.; Fonseca, F.C.A.; Silva, M.C.M.; Almeida-Engler, J.; Silva, M.S.; Lourenço-Tessutti, I.T.; Terra, W.R.; Grossi-de-Sa, M.F. (2020) . RNAi-mediated suppression of Laccase2 impairs cuticle tanning and molting in the cotton boll weevil (Anthonomus grandis ). Frontiers in Physiology , v. 11, p. 591569. https://doi.org/10.3389/fphys.2020.591569 . GRYNBERG, P.; Togawa, R.C.; Freitas, L.D.; Antonino, J.D.; Rancurel, C.; Costa, M.M.C.; Grossi-de-Sa, M.F.; Miller, R.N.G.; Brasileiro, A.C.M.; Guimaraes, P.M.; Danchin, E.G.J. (2020) . Comparative genomics reveals novel target genes towards specific control of plant-parasitic nematodes. Genes , v. 11, p. 1347. https://doi.org/10.3390/genes11111347 . IBARRA, L.N.; Alves, A.E.O.A.; Antonino, J.D.; Prado, G.S.; Pinto, C.E.M.; Soccol, C.R.; Vasconcelos, E.A.R.; Grossi-de-Sa, M.F. (2020) . Enzymatic activity of a recombinant β-1,4-endoglucanase from the cotton boll weevil (Anthonomus grandis ) aiming second generation ethanol production. Scientific Reports , v. 10(1), p. 5367. https://doi.org/10.1038/s41598-019-56070-1 . MOTA, A.P.Z.; Fernandez, D.; Arraes, F.B.M.; Petitot, A.S.; Paes-Melo, B.; Lisei-de-Sa, M.E.; Guimaraes, P.M.; Brasileiro, A.C.M.; Albuquerque, E.V.S.; Danchin, E.G.J.; Grossi-de-Sa, M.F. (2020) . Evolutionarily conserved plant genes responsive to root-knot nematodes identified by comparative genomics. Molecular Genetics and Genomics , v. 295, p. 1063-1078. https://doi.org/10.1007/s00438-020-01677-7 . MOURA, S.M.; Rossi, M.L.; Artico, S.; Grossi-de-Sa, M.F.; Martinelli, A.P.; Alves-Ferreira, M. (2020) . Characterization of floral morphoanatomy and identification of marker genes preferentially expressed during specific stages of cotton flower development. Planta , v. 252(4), p. 71. https://doi.org/10.1007/s00425-020-03477-0 . NORIEGA-VASQUEZ, D.D.; Arraes, F.B.M.; Antonino, J.D.; Macedo, L.L.P.; Fonseca, F.C.A.; Togawa, R.C.; Grynberg, P.; Silva, M.C.M.; Negrisoli, A.S.; Grossi-de-Sa, M.F. (2020) . Transcriptome analysis and knockdown of the juvenile hormone esterase gene reveal abnormal feeding behavior in the sugarcane giant borer. Frontiers in Physiology , v. 11, p. 588450. https://doi.org/10.3389/fphys.2020.588450 . NORIEGA-VASQUEZ, D.D.; Arraes, F.B.M.; Antonino, J.D.; Macedo, L.L.P.; Fonseca, F.C.A.; Togawa, R.C.; Grynberg, P.; Silva, M.C.M.; Negrisoli, A.S.; Morgante, C.V.; Grossi-de-Sa, M.F. (2020) . Comparative gut transcriptome analysis of Diatraea saccharalis in response to the dietary source. PLoS One , v. 15(8), p. e0235575. https://doi.org/10.1371/journal.pone.0235575 . PAES-MELO, B.; Lourenço-Tessutti, I.T.; Morgante, C.V.; Santos, N.C.; Pinheiro, L.B.; Jesus-Lins, C.B.; Silva, M.C.M.; Macedo, L.L.P.; Fontes, E.P.B.; Grossi-de-Sa, M.F. (2020) . Soybean embryonic axis transformation: combining biolistic and Agrobacterium -mediated protocols to overcome typical complications of in vitro plant regeneration. Frontiers in Plant Science , v. 11, p. 1228. https://doi.org/10.3389/fpls.2020.01228 . PAES-MELO, B.; Lourenço-Tessutti, I.T.; Paixao, J.F.R.; Noriega-Vasquez, D.D.; Silva, M.C.M.; Almeida-Engler, J.; Fontes, E.P.B.; Grossi-de-Sa, M.F. (2020) . Transcriptional modulation of AREB-1 by CRISPRa improves plant physiological performance under severe water deficit. Scientific Reports , v. 10(1), p. 16231. https://doi.org/10.1038/s41598-020-72464-y . RIBEIRO, T.P.; Basso, M.F.; Carvalho, M.H.; Macedo, L.L.P.; Silva, D.M.L.S.; Lourenço-Tessutti, I.T.; Oliveira-Neto, O.B.; Romano, E.; Lucena, W.A.; Silva, M.C.M.; Tripode, B.M.D.; Abreu-Jardin, T.P.F.; Miranda, J.E.; Alves-Ferreira, M.; Morgante, C.V.; Grossi-de-Sa, M.F. (2020) . Stability and tissue-specific Cry10Aa overexpression improves cotton resistance to the cotton boll weevil. Biotechnology Research & Innovation , v. 3, p. 15. https://doi.org/10.1016/j.biori.2019.12.003 . SANTOS, C.; Nogueira, F.C.S.; Domont, G.B.; Fontes, W.; Prado, G.S.; Habibi, P.; Santos, V.O.; Oliveira-Neto, O.B.; Grossi-de-Sa, M.F.; Jorrín-Novo, J.V.; Franco, O.L.; Mehta, A. (2020) . Proteomic analysis and functional validation of a Brassica oleracea endochitinase involved in resistance to Xanthomonas campestres . Frontiers in Plant Science , v. 11, p. 201. https://doi.org/10.3389/fpls.2019.00414 . Our Team Maria Fatima Grossi-de-Sa Team Leader Maria Fatima Grossi-de-Sa holds a bachelor's degree in Biological Sciences - biomedicine modality from the University of Brasilia (1979), a master's degree in Biological Sciences (Molecular Biology) from the University of Brasilia (1982), a doctorate (Doctorat Et Sciences) in Molecular Biology from the Université Paris VII-France (1987), and was a postdoctoral fellow at the Plant Genetic System-Ghent-Belgium (1988) and at the University of California in San Diego (1995-1996). She is the Lead Researcher at EMBRAPA Genetic Resources and Biotechnology (since 1989) and professor at the Catholic University of Brasília (since 2004). She is a CNPq productivity fellow (level 1A), member of CAPES International Advisory Committee (since 2007), full member (Agrarian Sciences) of the Brazilian Academy of Sciences (elected in 2011) and a member of the World Academy of Science -TWAS (elected in 2014). Among other awards and honors, she notably received the Scopus Award 2010 (Elsevier / CAPES) and the medal of the National Order of Scientific Merit (2018). She held the position of coordinator at the Biotechnology area and alternate member of CTC-ES at CAPES (2007-2014), and the presidency of the Brazilian Society of Biotechnology - SBBiotec (2008-2013 and 2016-current). She has experience in the field of Plant Genetics and Biotechnology, with an emphasis on Genetic Engineering and Plant Molecular Biology. The primary focus of her research is on the development of biotechnological products, using different strategies, including genome editing, aiming to increase tolerance and resistance to biotic and abiotic stress in plants. Morevoer, biotechnological tools are applied for the development of biopharmaceuticals. Her main research fields include: plant defense proteins, insecticidal proteins, plant-pest molecular interaction, and biotechnological assets applied to agribusiness. Carolina Vianna Morgante She is undergraduated in Biological Sciences at University of Sao Paulo - Biosciences Institute (1999). Carolina Morgante holds a master's and doctorate's degrees in Agronomy (Genetics and Plant Breeding) from the University of São Paulo (2003 and 2008, respectively). She is currently a researcher at Embrapa Semiárido and has experience in Genetics, focusing on Plant Genetics and Molecular. Diana Isolda Clotilde Fernandez She is currently a permanent Senior researcher at the French Research Institute for Development - Institut de Recherche pour le Développement (IRD, France) and remained until November 2020 at Embrapa-Cenargen. She has experience in Biochemistry, with emphasis on Molecular Biology, working on the following subjects: phytopathology, plant-pathogen interactions, plant immunity, nematodes, rust, rice, Coffea arabica , Hemileia vastatrix , Meloidogyne spp. Isabela Tristan Lourenço Tessutti Isabela is undergraduated in Biological Sciences from the University of Brasilia (2006), and holds a master's and a doctorate's degree in Molecular Biology from the same University (2008 and 2014, respectively). She was a post-doctoral fellow (2020) at the Institut National de Recherche pour l'Agriculture , l'Alimentation et l'Envrionment (INRA - Sophia Antipolis/France). Recently, she works at the Plant-Pest Interaction Laboratory at Embrapa Genetic Resources and Biotechnology, coordinated by Dr. Maria Fatima Gross-de-Sa. Her main research fields are: plant-pest interaction, plant resistance to biotic stress (nematodes and insects), and tolerance to abiotic stress (drought). She has expertise in: functional genomics for phytonematodes, insects, plants and bacteria; plant genetic transformation; gene (RNAi) silencing; heterologus expression of proteins using bacterial cells; functional characterization of plant promoters; spacial and temporal determinarion of gene expression using real-time PCR; genome editing using CRISPR methodology for biomass increase, drought tolerance and pest resistance. Leonardo Lima Pepino de Macedo Leonardo is undergraduated in Biological Sciences from the Federal University of Rio Grande do Norte (2005). He holds a master degree in Biochemistry from the same University (2007) and a doctorate's degree in Genomic Sciences and Biotechnology from the Catholic University of Brasília (2012). He has experience in Biochemistry and Molecular Biology, with expertise in the following areas: cloning and expression of proteins in heterologous systems; bioprospecting proteins with entomotoxic activity (vicillins, lectins, proteinase inhibitors and Cry toxins) aiming at the control of dipterous, lepidopteran and coleopteran insects; development of gene silencing strategies via RNAi for the control of insect pests. Maria Eugenia Lisei de Sa Maria Eugenia is undergraduated in Biological Sciences from Faculdades Metodistas Integradas Isabela Hendrix (1981), Master in Agronomy (Phytotechnics) from the Universidade Federal do Ceará (1984), PhD in Genetics and Biochemistry from the Universidade Federal de Uberlândia (2004) and post-doctorate fellow in Biotechnology at the Institute de Recherche pour le Développement-França (2013). She is Researcher (II) at the Minas Gerais Agricultural Research Corporation (EPAMIG) and currently works as a collaborative researcher at Embrapa Genetic Resources and Biotechnology -Cenargen. He has experience in the field of soybean breeding, with an emphasis on the development of soybean cultivars with characteristics suitable for human consumption. Her expertise lies on plant defense proteins (proteinase inhibitors, alpha-amylase inhibitors, lectins, defensins, osmotins); plant-pest molecular interaction; development of genetically modified plants for resistance to biotic stress (insects and nematodes) and tolerance to abiotic stress. Maria Cristina Mattar da Silva Maria Cristina is undergraduated in Biological Sciences from the Universidade Estadual Paulista Júlio de Mesquita Filho (1984) and from Universidade de Brasília (1987). She holds a master's degree in Biological Sciences (Molecular Biology) from the Universidade de Brasília (1992) and a doctorate's degree in Biological Sciences (Molecular Biology) from University of Brasília (2002). She is a Researcher at Embrapa Genetic Resources and Biotechnology since 1989. She is expert in plant molecular biology, working in the field of plant biotechnology for biotic and abiotic stress. The main focus of her researches are: evolution of molecules in vitro for selection of variants with improved activity; molecular studies of plant-pest interaction for insect resistance. Currently, she is a Member of the Brazilian Society of Biotechnology. FIND OUT MORE ABOUT OUR TEAM Contact Maria Fatima Grossi de Sá EMBRAPA Genetic Resources and Biotechnology W5 Norte Avenue (end) - P.O. Box 02372 - Postal Code 70770-917 - Brasília, DF - Brazil E-mail: fatima.grossi@embrapa.br Phone number: +55 61 3448-4705

The "INCT PlantStress Biotech" project therefore aims to integrate several Brazilian research groups and international partners with expertise in plant breeding and biotechnology. The primary goal of the network is to generate biotech assets applied to crop plants. About us In the present scenario, important crops naturally present low levels of drought tolerance and resistance to insect pests and nematodes. In this context, the complex mechanisms of response associated with environmental stresses (abiotic and biotic), and the small number of characterized and introgressed genes have been reported. Therefore, the search for new genes and molecules involved in tolerance or resistance to insect pests or drought stress is imperative. Hence, “INCT - Biotechnology applied to plant stresses” proposes the integration of several Brazilian research groups and international partners experts in plant physiology, transcriptomics, epigenetics, proteomics, bioinformatics, and functional genomics. The integrated research group aims to develop a multidisciplinary and multi-institutional network with national and international excellence to generate biotechnological assets applied to maize, soybean, and cotton. This project includes prospection, isolation, characterization, and functional validation of genes and molecules involved in resistance against pests (Meloidogyne spp., Helicoverpa armigera, and Spodoptera frugiperda) and water deficit tolerance. The generated assets will be applied in the development of biotechnology products, such as bio-insecticides and genetically modified soybean, maize and cotton plants. Furthermore, INCT research groups will not spare efforts to promote the qualification of students and professionals from different research areas, including biotechnology, genomics, and bioinformatics, either in Brazil or abroad. In the future, the INCT network can be used not only for the generation of biotechnological assets applied to drought stress and pests’ control in soybean, cotton and maize but also to other agronomic important traits (seed and fruit quality, nutrition value increase, and others) and important crops for the Brazilian agribusiness. See Publications Organizational Structure

Learn more about AL10, its main goals within the project and meet the laboratory's work team. AL 10 - Plant Transformation - Corn Laboratory Activities - PlantStress Biotech INCT Integrate data from monocotyledon transcriptomes (rice, corn, wheat, Musa and Sorghum ) submitted to water deficit, generated by mass sequencing (Illumina - HiSeq) in previous projects. Validate promoters by transient transformation by biolistics or Agrobacterium rhyzogenes in target plants for in vivo testing of promoters (soybean, cotton and corn). Validate promoters by stable transformation in target plants for in vivo testing of promoters identified as promising in previous tests (soybean, cotton and corn). Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Generate GM soybean, GM cotton and GM corn via strategies of overexpression or silencing of plant genes and evaluate the phenotype obtained regarding drought tolerance. Generate GM soybean, GM cotton and GM corn expressing sequences for silencing of insect genes and evaluate the phenotype obtained regarding the control of Helicoverpa armigera and Spodoptera frugiperda . Generate GM soybean, GM cotton and GM corn via toxin overexpression strategies and evaluate the phenotype obtained regarding the control of H. armigera and S. frugiperda . Phenotyping (greenhouse or field) corn, soybean and cotton populations, resulting from crossing with GM events to obtain proof of concept of drought tolerance and/or resistance to S. frugiperda , H. armigera or Meloidogyne spp. Newton Carneiro Team Leader Bachelor degree in Biology at Pontifícia Universidade Católica de Minas Gerais (1986), Master's in Plant breeding at Universidade Federal de Viçosa (1990) and Ph.D. in Plant Sciences at University of Arizona (1998). In 2015 participated as Visiting Scientist at University of Nebraska with RNAi. Experience in Genetics, focusing on Molecular Genetics and of Microorganisms, acting on the following subjects: biotic and abiotic stresses in maize and sorghum, genomic and transgenic. Contact Newton Portilho Carneiro EMBRAPA Milho e Sorgo E-mail: newton.carneiro@embrapa.br

Learn more about AL14, its main goals within the project and meet the laboratory's work team. AL 14 - Field Phenotyping - Private Sector Laboratory Activities - PlantStress Biotech INCT Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Phenotyping (greenhouse or field) corn, soybean and cotton population resulting from crossing with GM events to obtain proof of concept of drought tolerance and/or resistance to Spodoptera frugiperda , Helicoverpa armigera or Meloidogyne spp. Rafael Galbieri Team Leader Dr. Rafael is an agronomist graduated at the Federal University of São Carlos (UFSCar). He holds a master's degree in Agriculture from the Agronomic Institute of Campinas (IAC) and has a PhD degree in Tropical Agriculture from the Federal University of Mato Grosso (UFMT). Since 2007, he is a researcher (phytopathologist) at Instituto Mato-Grossense do Algodão (IMA). In 2008, he became the coordinator of the Department of Phytopathology/Nematology at IMA. His research focuses on the management of diseases and nematodes in cotton, soybean and castor bean crops, working in different areas, such as gentic, biological, chemical and cultural control of pathogens. Contact Rafael Galbieri Instituto Mato-grossense do Algodão. Rodovia BR070 km 266 Zona rural 78850-000 - Primavera do Leste, MT - Brazil - Caixa-postal: 149 Phone: (66) 34973301

Learn more about AL04, its main goals within the project and meet the laboratory's work team. AL 04 - Molecular Physiology Laboratory Activities - PlantStress Biotech INCT Sequencing and integrating transcriptomes of model plants (Arabdopsis , Setaria ) subjected to drought and high temperature during bioassays. Validate the function of plant genes potentially involved in the mechanisms of drought tolerance in Arabidopsis , rice or sepia plants via overexpression or silencing strategies. Generate PGMs from soybean, cotton and corn via strategies of overexpression or silencing of plant genes and evaluate the phenotype obtained regarding drought tolerance. Phenotyping (greenhouse or field) maize, soybean and cotton populations resulting from crossing GM events to obtain proof of concept of drought tolerance and/or resistance to Spodoptera frugiperda , Helicoverpa armigera or Meloidogyne spp. Generate PGMs from soybean, cotton and corn via strategies of overexpression or silencing of plant genes and evaluate the phenotype obtained regarding drought tolerance. Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Joaquim Albenisio Gomes da Silveira Team Leader Dr. Joaquim works with Plant Physiology, with emphasis on the efficiency of photosynthesis mediated by redox metabolism, photorespiration and N assimilation under conditions of abiotic stress. He holds a Master's degree from CENA and a doctorate from ESALQ/USP. He was a post-doctorate fellow at the Laboratory of Molecular Biology of Plant Stress at ITQB/Universidade Nova de Lisboa (2014-2015). He was one of the cofounders of the Brazilian Society of Plant Physiology, and was President of the Organizing Committee of the III Brazilian Congress of Plant Physiology. He participated in the creation and publication of the Brazilian Journal of Plant Physiology (1989), currently named Theoretical and Experimental Plant Physiology. Dr. Joaquim has published more than 150 scientic articles in international journals. He has trained over 29 doctorate students, 36 master students and has supervised 10 post-doctoral fellows. Nowadays, he is a professor at the Federal University of Ceará (UFC) anda a CNPq 1A researcher. He is also the vice-coordinator of the Graduate Program in Biochemistr at UFC and the head of Plant Metabolism Laboratory at the same university. Contact Joaquim Albenisio Gomes da Silveira Universidade Federal do Ceará Campus do Pici - Bloco 907 - CeP 60440-900 - Fortaleza - CE E-mail: silveira@ufc.br Phone number: +55 85 3366-9821

Learn more about AL11, its main goals within the project and meet the laboratory's work team. AL 11 - Biometrics Laboratory Activities - PlantStress Biotech INCT Integrate transcriptome data from monocotyledons (rice, maize, wheat, musa and sorghum) submitted to water deficit, generated by mass sequencing (Illumina-HiSeq) in previous projects. To identify in silico promoters responsive to biotic and abiotic stress. Assemble a vector bank with promoters driven the expression of reporter genes (GUS/GFP) in monocotyledons and dicotyledons. V alidate the function of plant genes potentially involved in drought tolerance mechanisms in Arabidopsis , rice or Setaria plants through strategies of gene overexpression or silencing. Organize, maintain and share an in vivo bank of innovation actives obtained in the project shared by INCT members. Laboratory Description The Plant Genomic and Breading Center was created in 2000 as a joint effort of the Plant Breeding and the Genomics Groups at FAEM UFPel. This effort has been working aiming for the balanced training of the next generation of plant breeders with molecular biology skills. The research group at PGBC is connected to the graduate Programs in Agronomy/Plant Breeding, Biotechnology and Seed Science and Technology. Research Lines Plant Breeding: grasses, legumes, solanaceous, liliaceae. Genetics resources, genome and plant biotechnology. Our Team Antonio Costa de Oliveira Team Leader Graduate in Agronomy from Universidade Federal de Pelotas (1986), MSc in Agronomy/Genetics and Plant Breeding from Universidade de São Paulo (1988), PhD in Genetics from Purdue University (1996) and Post-doctoral studies at University of Georgia/Department of Genetics (2007). Professor at UFPEl and CNPq Research Fellow level 1A. Has experience in Agronomy, focusing on Plant Improvement, acting on the following subjects: cereal genomics, rice, oats quantitative traits, breeding and variability. Has many scientific collaborative research works with USA, Italy, Spain, France, Portugal, England and Mexico. Camila Pegoraro Graduated in Agronomy from the Federal University of Pelotas (2008), Master in Food Science and Technology from the Federal University of Pelotas (2010) and PhD in Agronomy - area of concentration in Plant Genetic Improvement - from the Federal University of Pelotas, with period sandwich at the Università degli Studi di Padova (2012). She is currently an Adjunct Professor at the Federal University of Pelotas, an area of expertise in Plant Genetic Improvement. Luciano Carlos da Maia Agronomist with a PhD in Agronomy-Plant Breeding from FAEM/UFPel, where he currently works in the Department of Plant Science as Assistant Professor of Plant Breeding. He is a member of the Graduate Programs in Agronomy UFPel and Graduate Studies in Science and Technology of UFPel Seeds and collaborates with Research on Abiotic stress in rice in the Graduate Program in Plant Physiology - UFPel. Collaborates in the White Oat and Black Oat Breeding Program at the Genomics and Breeading Center - FAEM/UFPel. Develops studies of conventional breeding and quantitative genetics (rice and corn) and also studies in Molecular Biology and Bioinformatics, for the characterization of the genetic networks involved in abiotic stress (stress by salinity, by cold and iron in rice) and grain quality in rice . With corn, he has been carrying out studies for the phenotypic and molecular characterization of populations of Creole varieties from the southern half of RS, including studies with different genetic designs such as Full Brothers, Half Brothers and NC. He has experience in Bioinformatics (programming, databases and Linux OS) and Agricultural Experimentation. He was coordinator of PPG-Agronomia-UFPel between 2015-2017. Research Productivity Scholarship (PQ-2) in the notice 12/2017. Post-doctorate at the Institute of Molecular and Cellular Biology of Plants at the Universidad Politécnica de Valencia. Contact Antônio Costa de Oliveira Universidade Federal de Pelotas - Genomic and Breeding Center Campus UFPel - P.O. Box 354 - Postal Code 96160-000 - Capão do Leão, RS - Brazil E-mail: pcontato@cgfufpel.org Phone:+55 53 3275-7263

INCT Partnership The partnerships of INCT PlantStress Biotech have proven to be essential for its progress. These partnerships essentially originate from the network of collaborations that its members have in various areas of operation. Private Companies Companies collaborating for the development of research projects Read More Graduate Courses Graduate Courses with the participation of leading INCT PlantStress Biotech researchers Read More Associated Labs Laboratories that comprise the INCT PlantStress Biotech Read More International Collaborators International Universities and Research Institutes that collaborate in INCT PlantStrss Biotech Read More Private Companies Partner companies involved in the development of projects for the INCT PlantStress Biotech CORTEVA Support in research for the development of new soybean genotypes. IMAmt Support in research for the development of new cotton genotypes. Bio Bu reau Support startups to formalize their business and develop their company. SEMPRE SEMENTES Support in research for the development of new soybean genotypes. TOLVEG Applied research with microorganisms and plant bio-stimulating substances. SoluBio Ensure sustainability and produce more with less. HAPISEEDS Support in analyze level of positive plant interaction with bioinoculants. ABRAPA Support in research for the development of new cotton genotypes. PEPBioLabs Enzymes and peptides for laboratories and companies. INL Performing interdisciplinary research and deploy and articulating nanotechnology for the benefit of society. Graduate Courses Graduate courses, whose partnership with INCT PlantStress Bioech allows the use of infrastructure, resources, and themes for training development, both at the undergraduate and postgraduate levels. Thus, in addition to its scientific and technological character, INCT PlantStress Biotech trains human resources at all levels, either through postgraduate courses in which its researchers are involved or through scholarships for scientific initiation, technological development, and postdoctoral studies (from Capes, CNPq, and FAPs). Postgraduate courses with participation of lead researchers from INCT PlantStress Biotech: Graduate course in Genomic Sciences and Biotechnology - Universidade Católica de Brasília Graduate course in Molecular Biology - Universidade de Brasília Graduate course in Biotechnology - Universidade Estadual de Londrina Graduate course in Biological Sciences (Molecular Biology) - Universidade de Brasília Graduate course in Sciences (Microbiology) - Universidade Federal do Rio de Janeiro Graduate course in Vegetal Biotechnology and Bioprocess - Universidade Federal do Rio de Janeiro, Graduate course in Plant Biotechnology - Universidade Federal do Rio de Janeiro Graduate course in Biotechnology and Biodiversity - Universidade de Brasília Graduate course in Cellular and Molecular Biology - Universidade Federal do Rio Grande do Sul Graduate course in Biotechnological Processes - Universidade Federal do Paraná Graduate course in Biological Sciences (Genetics) - Universidade Federal do Rio de Janeiro Graduate course in Phytopathology - Universidade de Brasília Alan Buddie - CABI; Bakeham Lane; Egham; Surrey TW20 9TY; UK – E-mail: a.buddie@cabi.org Ana Zotta Mota - Centre de Recherche Développement Nestlé, 101 Av. Gustave Eiffel, 37390 Notre-Dame-d'Oé, France – E-mail: anazottamota@gmail.com Angharad Gatehouse - School of Natural and Environmental Sciences, Newcastle University, Newcastle NE1 7RU, UK - E-mail: a.m.r.gatehouse@ncl.ac.uk David Bertioli - Department of Crop and Soil Science, University of Georgia, Athens, 30602 GA, USA - E-mail: bertioli@uga.edu Deepak Sharma - Department of Genetics and Plant Breeding, Indira Gandhi Krishi Vishwavidyalaya (IGKV) - Raipur, 492012, India – E-mail: deepak1962@igkv.ac.in Diana Fernandez - IRD, CIRAD, Université de Montpellier, IPME, F-34398 Montpellier, France – E-mail: diana.fernandez@ird.fr Dirk Inze – Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium - E-mail: dirk.inze@psb.vib-ugent.be Etienne GJ Danchin - INRAE, Université Côte d'Azur, CNRS, Institut Sophia Agrobiotech, Sophia-Antipolis, France – E-mail: etienne.danchin@inrae.fr Franc-Christophe Baurens - CIRAD, UMR AGAP Institut, F-34398 Montpellier, France – E-mail: franc-christophe.baurens@cirad.fr .  Gilbert Joseph Engler - INRAE, Université Côte d'Azur, CNRS, Institut Sophia Agrobiotech, Sophia-Antipolis, France – E-mail: gilbertengler@gmail.com Henry Daniel - Department of Basic and Translational Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104-6030, USA - E-mail: hdaniell@upenn.edu Jan Leach - Department of Agricultural Biology, Colorado State University, Fort Collins, Colorado, USA – E-mail: jan.leach@colostate.edu Janice de Almeida Engler - Institut National de la Recherche Agronomique – INRAE, France – E-mail: janice.de-almeida@inrae.fr Juan Luis Jurat-Fuentes - Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN 37996, USA – E-mail: jurat@utk.edu Kazuo Nakashima – Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki, 305-8686, Japan - E-mail: kazuo.nakashima@affrc.go.jp Laurens Pauwels – Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium – E-mail: laurens.pauwels@psb.vib-ugent.be Lucia Colombo - Dipartimento di Bioscienze, Università Degli Studi di Milano, Via Celoria 26, 20133, Milan, Italy – E-mail: lucia.colombo@unimi.it Luis Willian Arge - Department of Agronomy and Plant Genetics, University of Minnesota, Saint Paul, MN 55108-6026, USA – E-mail: l.willianpacheco@gmail.com Martin Crespi - Université Paris-Saclay, CNRS, INRAE, Université Evry, Institute of Plant Sciences Paris-Saclay, France - E-mail: martin.crespi@cnrs.fr Martin Kater - Department of Biosciences, Università degli Studi di Milano, via Celoria 26, 20133, Milan, Italy - E-mail: martin.kater@unimi.it Nelson Saibo - Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), Oeiras, Portugal – E-mail: saibo@itqb.unl.pt Nicolas Roux - Bioversity International, Parc Scientifique Agropolis II, Montpellier, France – E-mail: n.roux@cgiar.org Pat Heslop Harrison - University of Leicester, Department of Genetics and Genome Biology, Institute for Environmental Futures, Leicester LE1 7RH, UK – E-mail: pat.heslop-harrison@bbsrc.ac.uk Peggy Ozias - Department of Horticulture and Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Tifton, GA, 31793, USA – E-mail: pozias@uga.edu Ping He - Department of Molecular, Cellular, & Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA – E-mail: pinghemi@umich.edu Randall Wisser - Institut National de la Recherche Agronomique – INRAE, France - E-mail: randall.wisser@inrae.fr Raquel Chan - Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional Del Litoral, Santa Fe, Argentina - E-mail: rchan@fbcb.unl.edu.ar Rod Wing - Center for Desert Agriculture, Biological and Environmental Sciences & Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia – E-mail: rod.wing@kaust.edu.sa Shuangxia Jin - National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, P.R. China - E-mail: jsx@mail.hzau.edu.cn Soraya Leal-Bertioli - Center for Applied Genetic Technologies, University of Georgia, Athens, 30602, GA, USA – E-mail: sorayab@uga.edu Todd Michael - The Plant Molecular and Cellular Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA - E-mail: tmichael@salk.edu Yiping Qi - Department of Plant Science and Landscape Architecture, University of Maryland, College Park, Maryland, USA – E-mail: yiping@umd.edu Zhiyong Wang - Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, USA – E-mail: zwang@carnegiescience.edu International Colaborators